查询词典 primer

This is the "c primer" This book supporting source code, and I hope to be helpful.

这是《c++primer》这本书的配套源代码，希望对大家有帮助。

According to the gallus colony stimulating factor(colony-stimulating factor,CSF)cDNA sequences published by GenBank,a pairs of specific primer was designed,the local dorking was injected 100 μg/mL ConA,total RNA was extracted from feeding 24 hours lymphocyte from spleen,then dorking colony stimulating factorcDNA fragment was cloned by RT-PCR technology.

根据GenBank中报道的红原锦鸡集落刺激因子(Colony-stimulatingfactor，CSF)cDNA序列，利用Primer Premier5.0软件设计一对特异性引物，对本地肉鸡注射100μg/mL ConA试剂，饲养24 h后，取脾脏提取淋巴细胞总RNA，利用RT-PCR技术克隆集落刺激因子的cDNA片段。

According to the gallus colony stimulating factor (colony-stimulating factor, CSF) cDNA sequences published by GenBank, a pairs of specific primer was designed, the local dorking was injected 100 μg/mL ConA, total RNA was extracted from feeding 24 hours lymphocyte from spleen, then dorking colony stimulating factor cDNA fragment was cloned by RT-PCR technology.

根据GenBank中报道的红原锦鸡集落刺激因子(Colony-stimulatingfactor， CSF)cDNA序列，利用Primer Premier5.0软件设计一对特异性引物，对本地肉鸡注射100μg/mL ConA试剂，饲养24 h后，取脾脏提取淋巴细胞总RNA，利用RT-PCR技术克隆集落刺激因子的cDNA片段。

2 STR-PCR We analysed the results of STR with the silver dye in the beginning. The silver dye of STR analysis on a sporadic DMD family shows the band confirmation is subjective by eyeballing. It can not detect the small repeats and the size of polymorphic fragments. For limitation of the silver dye, we analysed the band size of STR with the SAGAGT Genotyping System. Primer are designed according to the nucleotide sequence of five STR markers (located in 5' terminus and intron 44, 45, 49, 50) of dystrophin gene. The 5' end of the forward primer are attached a M13 tailed primer which conjugated to the infrared flourescent dye.

根据dystrophin基因5个多态位点（44、45、49和50号内含子以及5'端启动子区的序列）设计引物，在这些STR的上游引物5'端加一可被红外线荧光物质IRDye700识别标记的M13fw的尾：CAC GACGTT GTA AAA CGA C 通过PCR扩增出重复序列本身，经高分辨率的变性聚丙烯酰胺凝胶电泳后，利用红外激光遗传分析系统及SAGA〓基因分型软件对凝胶上的扩增产物及内标的位置，进行扫描分析，确定产物片段的大小。

The invention discloses a method to analyze fish genetic information with mitochondria DNA D-loop controlling zone, which comprises the following steps:(1) extracting genom DNA and mitochondria DNA of fish;(2) designing simple primer in fish mitochondria DNA D-loop controlling zone; possessing twenty one basic groups CAC CCY TRR CTC CCA AAG CYA in upstream primer MitD1-F; possessing twenty three basic groups GGT GCG GRK ACT TGC ATG TRT AA in downstream primer MitD1-R;(3) proceeding PCR reaction under the function of primer;(4) choosing agarose jel electrophoresis;(5) checking sequence for augment mtDNA D-loop gene fragment.

本发明公开了一种利用线粒体DNA D-loop控制区进行鱼类遗传信息分析的方法。它包括如下步骤：(1)分别提取鱼类基因组DNA和线粒体DNA；(2)在鱼类线粒体DNA D-loop控制区设计简并引物：其上游引物MitD1-F有21个碱基：CAC CCY TRR CTC CCA AAG CYA；下游引物MitD1-R有23个碱基：GGT GCG GRK ACT TGC ATG TRT AA；(3)在引物的作用下进行PCR反应；(4)琼脂糖凝胶电泳；(5)对扩增的mtDNA D-loop基因片断进行测序。

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These things are normatively defined in http://www.w3.org/TR/2004/REC-rdf-primer-20040210/#ref-rdf-concepts

这些事物在http：//www.w3.org/TR/2004/REC-rdf-primer-20040210/#ref-rdf-concepts

Methods Four pairs of these primers that would be amplified in simulium COI gene were designed by Primer Premier 5.0, the results of PCR would be investigated through agarose gel electrophoresis.

运用Primer Premier 5.0软件设计4对蚋科COI基因的PCR扩增引物，通过PCR扩增后进行琼脂糖凝胶电泳检测实验。

Very porous surfaces such as new plaster, concrete, gypsum board, plaster board, chipboard and block board should be sealed with undiluted Primer/Sealer and allowed to dry for + 5 Hours.

非常多孔表面譬如新膏药、混凝土、石膏板、膏药板、chipboard 和块板应该被密封与未稀释的Primer/Sealer 一起烘干5 个小时。

The method is just like the QuikChange site-directed mutagenesis method except for its special primer. This pair of primers is composed of a long primer and a short primer which is central overlapped.Both of the Tm of 5\'sequence in long primer and Tm of 3\'sequence in short primer are above 66℃.The Tm of short primer is almost half of that of long primer.It is convenient to perform insertion or substitution mutation of DNA fragments larger than 20bp,In addition,it is highly applicable in research about the deletion mutation of DNA fragments larger than 2000bp.

我们实验室一直在寻找一种简单高效的可以用于基因的删除、插入及替代突变的方法，通过大量研究和实验发现，可以使用一种基于反向PCR原理，使用全新的引物设计的突变方法——&COP&突变法，该方法采用与QuikChange点突变法相同的操作步骤，其策略是设计一对中心重合的引物，长引物的5\'端序列和3\'端序列的Tm值均不低于66℃，短引物Tm值约为长引物一半，且与长引物中心重合互补。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。