查询词典 primed

She was well primed with a good load of Delahunt's port under her bellyband.

看啊，清晨的微曦。她那肚带下面灌满了德拉亨特的红葡萄酒。

"I would say the ice in the vicinity of the North Pole is primed for melting, and an ice-free North Pole is a good possibility," Sheldon Drobot, a climatologist at the Colorado Center for Astrodynamics Research at the University of Colorado, said by email.

"我想说的是在北极点附近的冰将要融化，并很可能出现一个无冰的北极点。"谢尔登多波特，一位在科罗拉多大学科罗拉多天文动力研究中心的气侯学家在邮件中说。

Have a fully outfitted first aid kit, a primed fire hydrant, and a toolbox in your car.

有一个完全配备急救包，引消防栓，和一个工具箱，在您的汽车。

Once a individual herself, Salander is today primed to fisticuffs back.

而作为受害者本身，Salander正准备进行反击。

Lot Ceiling Value at 100% of Lot Annual Volume, Item Pricing-DDP Delivered 10% Volume, Extended Price(10% Volume), Volume Segment Sub Total Exterior Jamb-Head Jamb Entry Door Finger-jointed Primed, Head Jamb Patio Door, Inswing Blank Entry Door Jamb RH or LH Finger-jointed Primed, Outswing Blank Entry Door, Blank ENtry Door Jamb, Brickmold, Inswing Brickmold, Flat Jamb Header, Veneered Flat Jambs Header, Stop-FJ, Stop-Solid, Split Jambs, Split Jamb Header, Casing-FJ, Finger-jointed Raw, Stiles and Rails, Interior Rail, Bi-Fold Siles-FJ, Bi-fold Stile-Multi F/J, Bi-Fold Stiles-LVL or Solid, LVL Pine Veneered Wrapped or Clear Solid, Interior Panel Doors-6 panel oak Door Single Hip, Interior Frame Vertical Leg(rabbited 16mm x 29mm), Interior Frame Header, Exterior Latch Stile, Exterior HInge Stile, Exterior Hinge Stile Stepped

地段价值上限为100 %地段每年出版一卷，项目定价铂交付10 %数量，延长价格( 10 %体积)，体积部分计外观金马头金马进入大门手指缝催芽，金马头门，天井， inswing空白大门进入金马铑或lh的手指相接催芽， outswing空白进入大门，进入空白金马门， brickmold ， inswing brickmold ，单位补报头，胶合板单位门窗头，停fj的，停止固体，门窗分裂，分裂金马头，套管fj的，手指缝生吃，罗威和钢轨，内政部长铁路，双向折叠石英- fj的，双向折叠stile -多种纤维/ j部，双折罗威拉特或固体，拉特松木胶合板包裹或固体清楚，内政部小组门-6小组橡木门单髋部，内务部内垂直腿( rabbited为16mm ×支)，内政部长头内，外部锁存stile ，外饰stile铰链，铰链外stile阶梯

DNA fingerprinting for Taraxacum mongolicum and its adulterants of six species of Compositae was demonstrated with random primed polymerase chain reaction including arbitrarily primed PCR and random amplified polymorphic DNA.

采用分子生物技术包括任意引物聚合酶链式反应和随意引物扩增的多态性DNA方法扩增蒲公英及其6种土公英混淆品的基因组DNA，获得清晰可靠的DNA指纹图谱。

The early affective priming ERPs effect was characterized by a longer P2 peak latency and decreased P2 peak amplitude for control condition as compared to primed and unprimed conditions; the late affective priming ERPs effect consisted of a shorter P3 latency and attenuated P3 peak amplitude for primed condition as compared to unprimed condition.

5在阈下启动任务中，对于P2、P3成分的潜伏期与波幅，述情障碍组与非述情障碍组之间均不存在显著差异；在阈上启动任务的两个SOA水平中，述情障碍组的P2成分和P3成分波幅均显著小于非述情障碍组的波幅。

DCs acquired by our reformed methods express both CD83 and CD 14 molecules highly, and have a higher density than other domestic reports. The higher TNF in DCs culture medium of HC patient suggests DCs in patient still have antigen presenting ability and by optimization the culture medium would improve its presenting ability and have a potential value in design and application individual vaccine. Although antigens pulsed DCs have a decrescent antigen presenting ability but BCG HSP70 could induce its mature and improve its presenting ability. Suggests BCG HSP70 would be a useful mature inducer. Lymphocytes primed by DCs based HC vaccine have the specific cytotoxicity against HCC lines. The CTL after freezing and anabiotic could prophylaxis and therapy HC xenograft on nude mouse. The results also suggests that CD4〓 lymphocytes play a important role in HC with a good differentiation and would be useful in treatment this kind of HC. After being activated by Peptide LLNQHACAV of hAFP and apoptotic HCCs pulsed DCs respectively, the culture medium of activated lymphocytes both contains a high level Th1 cytokines IL-12 and TNF. Primed lymphocytes appeared a characteristic of NK cells. DCs not only inhibited the growth of human HCC and other cancer cells in vitro but also prevented the growth of HC xenograft on nude mouse in vivo. There are at least three kinds of mechanism playing important role in DC based vaccine ,there are inhibition of DCs, HC specific CTL and cytokines pathway.

诱导出的DC共同表达CD83和CD14分子，CD83分子表达明显高于国内报道；肝癌患者DC培养上清中TNF水平高于健康人，提示肝癌患者DC仍具一定的抗原呈递能力，适当调控可使其行使APC功能，以期在肝癌个体化疫苗中发挥作用；DC负载肝癌可溶性抗原后，抗原呈递能力降低，BCG HSP70可促进DC成熟，增加其抗原呈递能力，预示BCG HSP70有可能成为促进DC活化和成熟的另一重要分子；肝癌DC疫苗在体外诱导肝癌特异性淋巴细胞，活化的淋巴细胞在体外对肝癌细胞的杀伤以特异性CTL为主，同时分泌较高水平Th1型细胞因子IL-12和TNF，并抑制4种肝癌细胞生长；冷冻复苏后的肝癌特异性淋巴细胞可以预防和抑制人肝癌裸鼠皮下移植瘤，提示DC负载肝癌可溶性抗原后诱发的MHC-Ⅱ类限制性CD4〓T细胞有可能在分化程度高的肝癌治疗中发挥作用；用DC和HLA-A2〓DC分别负载凋亡肝癌细胞和hAFP218-226位LLNQHACAV HLA-A2限制性九肽，在体外诱导肝癌特异性淋巴细胞，活化后的CTL细胞分泌较高水平的Th1型细胞因子IL-12和TNF，并具较强杀伤活性，此CTL同时具备NK细胞特征；DC对肿瘤细胞的抑制作用可能是通过吞噬实现的，Fas-L在DC抑制中也起一定作用；DC对人肝癌裸鼠皮下移植瘤的抑制率为97％；在肝癌DC疫苗的作用中，至少联合3种以上机制，即通过DC的直接作用、肝癌特异性CTL和细胞因子途径直接或间接地杀伤和抑制肝癌细胞。

Get high purity DCs by Cultured plastic-adherent monocytes isolated from healthy human peripheral blood with GM-CSF and IL-4 for 7 days. To observe the morphology of DCs by inverted phase contrast microscope ,electron microscope and laser confocal microscope. Analyse phenotype of DCs with flow cytometry. Investigate the endocytosis ability of DCs as a group by Horseradish peroxidase endocytosis assay. To appraise allogeneic mixed lymphocytes reaction of DCs by MTT reduction assay. Analyse the levels of IL-12 and TNF in liquids of cultured medium by ELISA and MTT reduction assay respectively. Soluble antigens of HCCs was obtained by 3 freeze-and-thaw cycles. Biological characteristics of HC soluble antigens pulsed DCs were monitored by flow cytometry. According to MTT reduction assay estimated the cell proliferation of self lymphocytes activated by HC antigens pulsed DCs. Get high purity BCG HSP 70 protein by SDS-PAGE electrophoresis and determined its biological activity with ELISA. Analyse phenotype of antigen pulsed DCs primed by BCG HSP70 with flow cytometry. By MTT reduction assay estimated the cell proliferation of self lymphocytes and the MLR of DC based vaccine. Analyse expression of HLA-DR molecule on surface of HCC lines. The IFN-γ mRNA in lymphocytes after actived by DC vaccine and the Fas-L expression on DC and DC vaccine primed lymphocytes were detected by in situ hybridization and flow cytometry respectively. Specific cytotoxity lysis of T lymphocytes and nonspecific inhibition of liquids in culture medium against HCC lines were also tested. Detect expression of hAFP on four HCC lines with Cell-ELISA. Induce apoptosis of HCCs with actinomycin-D. Interaction of DCs and apoptotic cells was observed under transmission electron microscope. Growth inhibition test of DC against HCC lines was also performed. Establish the nude mouse model bearing human HC xenografts and indentify the characteristic of tumour by histochemistry and immunohistochemistry techniques. Prevent and treat transplanted human HC on nude mouse with Freezing and anabiotic HC specific lymphocytes.

用GM-CSF和IL-4从健康人外周血诱导DC；分别用倒置相差显微镜、电子显微镜及激光共聚焦显微镜观察DC形态；流式细胞术检测DC表型；HRP吞噬实验测定DC的群体内吞能力；MTT法检测同种异体混合淋巴细胞反应；ELISA法和MTT法分别测定DC培养上清液中IL-12和TNF水平；冻融法制备肝癌细胞可溶性抗原；流式细胞术检测负载肝癌可溶性抗原后DC的生物学特性；MTT法检测DC负载肝癌抗原后对自身淋巴细胞增殖的影响；SDS-PAGE制备电泳纯化BCG HSP70并鉴定纯度，ELISA测定活性；流式细胞术检测负载抗原DC经BCGHSP 70活化后的表型；MTT法检测肝癌DC疫苗对自身淋巴细胞增殖的影响和混合淋巴细胞反应；流式细胞术检测肝癌细胞表面HLA-DR表达；MTT法检测肝癌DC疫苗对自身淋巴细胞的活化；原位杂交法检测肝癌DC疫苗活化后的淋巴细胞IFN-γmRNA表达；流式细胞术检测DC和肝癌DC疫苗活化后淋巴细胞表面Fas-L；MTT法分别检测肝癌DC疫苗活化的淋巴细胞和其培养上清对肝癌细胞的特异性杀伤和非特异性抑制作用；Cell-ELISA检测人肝癌细胞hAFP表达；MTT法检测负载AFP表位肽和凋亡肝癌细胞DC对自身淋巴细胞增殖的影响；ELISA法和MTT法分别测定活化后淋巴细胞培养上清中TNF和IL-12水平；肝癌细胞凋亡的诱导和检测；DC吞噬凋亡肝癌细胞后的电子显微镜观察；DC对肝癌细胞的生长抑制试验；人肝癌裸鼠皮下移植瘤动物模型的建立及其组织学和免疫组织化学鉴定；DC及肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤；冻存和复苏后的肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤。

On the other hand, mice primed with all the microsphere formulations showed higher anamnestic responses than those primed with soluble tetanus toxoid. Higher anamnestic response means stronger protection from infection.

另一方面，用微球免疫过的小鼠再次接受低剂量抗原时，所产生的免疫记忆抗体高于用破伤风类毒素溶液免疫过的小鼠，说明微球具有更强的免疫保护力。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。