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Primary applied study on treatment of wastewater containing copper by single cell alga is mainly carried out in this article.?

本文主要对单细胞藻类处理含铜废水进行了初步应用基础研究。

Results Primary RPCs extracted from the neural retina demonstrate certain biological characteristics: the cell population could form neurospheres and younger adherent cells expanded in clusters.

结果 从兔胚胎视网膜神经部分离出的原代视网膜前体细胞具有明显的生物学特性：所培养细胞可形成悬浮生长的细胞团，刚贴壁的细胞成簇状生长，这些细胞用免疫细胞化学染色神经上皮干细胞蛋白表达呈阳性。

Objective To explore the clinical significance of red blood cell polyamine in the diagnosis of primary hepatic carcinoma.

目的 探讨红细胞多胺对诊断原发性肝癌的临床价值。

Furthermore, preliminary work also performed to examine whether PI3K/AKT signal transduction pathway was activated in the process of refractory leukemia development. Materials and methods An immortalized human bone marrow stromal cell line, HS-5, was introduced to establish a bi-phase culture system for the cultivation of B-lineage precursor leukemia cells. ELISA and RT-PCR were used to investigate the expression of VEGF and its receptors in the leukemia cell lines and primary childhood leukemia cells in different treated groups. Flow cytometory method and immunofluorescent staining were employed to examine the apoptosis signals both in the VP16 treated and untreated leukemia cells. Western blot was utilized to explore the PI3K/AKT activated status in the drug induced or uninduced leukemia cells and lymphocytes from healthy donors.

材料和方法使用来源于人类骨髓基质细胞的细胞株HS-5作为滋养层细胞进行急性淋巴细胞性白血病细胞的体外培养，通过细胞生物学和免疫学方法评估培养体系并鉴定出难治性白血病细胞克隆；以ELISA和RT-PCR方法检测急性白血病细胞株和患儿白血病细胞VEGF及其受体的表达，了解不同治疗阶段VEGF及其受体的表达状况，并结合临床指标进行分析，明确VEGF及其受体在白血病发生过程中的作用；流式细胞仪和免疫荧光染色法对正常健康儿童、初发白血病患儿、复发白血病患儿及缓解后患儿进行凋亡因子检测和分析，初步阐明难治性白血病抗凋亡形成的原因；蛋白印记分析检测PI3K/AKT信号传导通路在健康儿童、初发白血病和复发白血病患儿的表达，初步了解难治性白血病形成的分子生物学机制。

Examine at present division the large check meter that deploys top-ranking modernization implement equipment and professional identifier just, among them advanced title is 1 person, intermediate title 17 people, primary 20 people, at present section office is had the United States is elegant the AEROSET that earths up company production is large full automatic biochemical analyzer, I2000SR, AXSYM is full automatic Beckman of immune analyzer, United States - the LH750 of the company reachs Kurt GenS is full automatic hematic cell analyzer, United States is strong unripe VITROS-250 emergency call does type biochemistry, biosite Triage heart straightens / the heart declines laser of double color of analyzer of chroma of diagnostic appearance, IMX medicaments, BD sheds type cell appearance, France VITEK2 of Mei Li dust is full automatic senior expert analyses microbial appraisal, medicine quick fast blood fosters system, Bact ALERT3D current of system, blood changes analyzer.

目前检验科配备一流现代化的大型检验仪器设备及专业检验人才，其中高级职称1人、中级职称17人，初级20人，目前科室拥有美国雅培公司生产的AEROSET大型全自动生化分析仪、i2000SR、AXSYM全自动免疫分析仪、美国贝克曼-库尔特公司的LH750及genS全自动血细胞分析仪、美国强生VITROS-250急诊干式生化分析仪，Biosite Triage心梗/心衰诊断仪、IMX药物浓度分析仪、BD双色激光流式细胞仪、法国梅里埃VITEK2全自动微生物鉴定、药敏高级专家分析系统、Bact ALERT3D快速血液培养系统、血液流变分析仪等先进仪器。

Methods Hepatic cells were obtained from fetal rats by means of mechanical separation and hanging-drop culture, and after two days of primary culture, the medium was changed for further cell culture in the presence of 20% fetal bovine serum (containing glucose 25ml/L, mycillin 100 U/ml, pH 7.6), 10 mmol/L nicotinamide, 1 mg/L insulin, affix N2, basic fibroblast growth factor, stem cell factor, epidermal growth factor and leukemia inhibitory factor.

采用机械分离法结合悬滴培养法获得胎肝干细胞，原代培养2d后改变培养基，添加含20%胎牛血清的DMEM（含25ml/L葡萄糖，100U/ml青链霉素，pH值7.6），添加烟酰胺10mmol/L，胰岛素1mg/L，N2添加剂，碱性成纤维细胞生长因子、表皮生长因子、干细胞因子、白血病抑制因子各20μg/L诱导分化为nestin阳性细胞。

To investigate apoptosis biological characteristics in primary esophageal small cell carcinoma and small cell lung cancer.

小细胞癌是一种恶性度及死亡率均较高的少见恶性肿瘤。

Objective To investigate whether 18F-fluorodeoxyglucose uptake within the primary tumor correlates with cell proliferation in non-small cell lung cancer.

目的 探讨非小细胞肺癌标准摄取值与肿瘤增殖细胞核抗原表达的关系。

Objective To study tumor angiogenesis in primary lung cancer and its relation with lymph node metastasis, tumor cell apoptosis, and cell proliferation.

目的 检测肺癌组织中的血管生成情况，并探讨其与肺癌淋巴结转移、肿瘤细胞凋亡及增殖的关系。

Cell-cycle synchronization between the donor cell and recipient oocyte determines the embryo development in nuclear transfer. In the present study, we microinjected primary spermatocyte into the perivetelline space of oocyte. 37% pairs fused after electric stimuli and the resulting oocytes were culture for 2 h in MEM with or without CB.

在本研究中，我们将小鼠的初级精母细胞显微注入MI卵母细胞的透明带下，经直流电脉冲作用后有37％的初级精母细胞融入卵母细胞，然后将融合的卵母细胞分成两组在MEM和含有CB的MEM培养液中分别培养，2小时后将在CB培养液中培养的卵母细胞转入正常培养液中。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。