查询词典 prickle cells

The expressions of DNA-PKcs and Id2 in SP cells were strikingly stronger than those in non-SP cells, The localization of DNA-PKcs in SP cells was in whole nucleus but that of the protein in non-SP cells was only in nuclear membrane and nucleoplasm nearby.

SP细胞DNA-PKcs表达的阳性强度明显高于非SP细胞；SP细胞Id2表达的阳性强度明显高于非SP细胞；SP细胞的p16表达的阳性强度明显低于非SP细胞；SP细胞FHIT的表达为阴性或微弱阳性；SP细胞NFκB/p65表达的阳性强度明显低于非SP细胞。

The main components of every ommatidium of compound eye were completed in the larval stages. An ommatidium composed of cornea, four corneagenous cells, four cone cells and retinular cells. The sensitization system of an ommatidium included 11 retinular cells, of which four located at the distal part of the ommatidium; seven formed the proximal main part of the retina. Their structures are different.

小眼的感光系统由11个小网膜细胞组成，且与成体一样，11个小网膜细胞已经分化成4+7两层，其中4个小网膜细胞构成了感光部分的远端，7个小网膜细胞构成感光系统的近端主体，远端和近端的小网膜细胞在结构上也有明显差异。

The adenovirus plasmid was identified by PacI digestion and transfected into 293A cells to package a recombinant adenovirus which expressed the Fhit protein. Furthermore, the adenovirus rAd-Fhit was infected into colon cancer cells,and the expression of the ectogenic protein was detected by Western blotting. Finally, the proliferation of colon cancer cells was observed in adenovirus-infected cells by the MTT assay.

经PacI酶切鉴定正确后，将重组腺病毒质粒转染293A细胞获得表达Fhit蛋白的重组腺病毒rAd-Fhit，将获得的重组腺病毒感染结肠癌细胞，采用蛋白印迹法检测外源Fhit蛋白的表达，并进一步观察其对细胞增殖能力的影响。

RESULTS A eukaryotic expression system for high expression humanmutantCD59 were successfully set up : The recombinant PALTER-MAX plasmid containing human mutantCD59 cDNA and PCDNA plasmid were co-transfected into CHO cell by cation lipoid mediating method ;and the cells were grown in F12 medium containing 400ug/ml G418 for 14 days, positive clones were grown in RPMI1640 medium to get stable expressing cell lines . Highly expressing clones were selected by flow cytometry ,and were named PALTER-CD59-CHO1PALTER-CD59-CHO2 . Flow cytometry indicated that expression rates of PALTER-CD59-CHO1 and PALTER-CD59-CHO2 were 53.7%and 54.5%. Further more, Stable highly expressing CHO cell lines were more detected by immunocytochemistry and immunofluorescence technology . PALTER-CD59 -CHO1 and PALTER-CD59-CHO2 were grown in RPMI1640 to get a large of cells . CD59 protein were obtained by spalling PALTER- CD59- CHO1 and PALTER - CD59 - CHO2 cells . Stable highly expressing cells were further validated by SDS-PAGE, immunoblot analysis and solid enzyme immunoassay . PALTER - CD59 - CHO1 and PALTER - CD59 - CHO2 were glycated in RPMI1640 of 50mM ribose for 72 hours .BCECF releasing test indicted that the releasing rate of PALTER-CD59-CHO1 or PALTER-CD59-CHO2 was less high than PALTER-CHO ,and the releasing rate of glycated PALTER-CD59 -CHOI or PALTER-CD59-CHO2 was higher than unglycated ones . PALTER -CD59-CHO1 and PALTER -CD59 -CHO2 were glycated in RPMI1640 of 50mM ribose for 72 hours .BCECF releasing test indicted that the releasing rate of PALTER - CD59 - CHOI or PALTER - CD59 - CHO2 was less high than PALTER-CHO ,and the releasing rate of glycated PALTER-CD59-CHO1 or PALTER - CD5 9-CHO2 was higher than unglycated ones .

结果 成功构建突变人CD59的真核细胞表达系统：运用阳离子脂质体介导法将含有突变人CD59的PALTER—MAX重组质粒与PCDNA共转染入CHO细胞：用含有400ug／mlG418的F12培养基培养14天，筛选出稳定阳性表达克隆，RPMI1640培养基扩增获得稳定表达细胞株，并用流式细胞术进一步筛选出高效表达细胞株分别命名为PALTER—CD59—CH01、PALTER—CD59—CH02，表达率分别为53.7％、54.5％；应用免疫组化方法、免疫荧光技术进一步鉴定阳性细胞株；RPMI1640培养基大量扩增PALTER—CD59—CH01、PALTER—CD59—CH02细胞株，裂解细胞得到CD59蛋白质；通过SDS—PAGE凝胶电泳技术、免疫印迹技术、固相酶联免疫吸附试验验证了这两中文摘要个阳性细胞株CO59蛋白的高效表达；50mM核糖培养72小时，获得突变人CD59糖化细胞株，BCECF染料释放试验结果显示，PALTER一CD59一CHOI、pALTER一CD59一CHOZ细胞较PALTER一CHO细胞染料释放率低，未糖化PALTER一CD59一CHOI、PALTER一CD59一CHOZ细胞比较糖化后细胞染料释放率低。

The results indicate that the longitudinal nerve fibers in cerebral ganglia and a few of small cells in the surface layer of cerebral ganglia present NOS positive reaction. Abundant NOS positive small cells are in the surface layer of pedal ganglia, and abundant transverse positive nerve fibers in the center of pedal ganglia. A large number of transverse positive nerve fibers are in the center of visceral ganglia; abundant positive small cells and nerve fibers are in two anterior lobes; a few of positive small cells and many encircled positive nerve fibers are in the posterior lobe; a large number of radiate positive nerve fibers are in the lateral lobes.

组织化学显示，存在NOS的部位如下：脑神经节内纵行的神经纤维和表层的少量小细胞；足神经节表层的大量小细胞，中央大量水平分布的神经纤维；脏神经节中部大量水平分布的神经纤维，前叶内大量小细胞和神经纤维，后叶内少量小细胞和许多环行神经纤维，侧叶内大量似放射状分布的神经纤维；脑足和脑脏神经索内的神经纤维。

IFN-γcomes from antigen activated T cells and NK cells, which have function on immunoregulation and effect factor in anti-virus and anti-tumor including inducing activation of cytotoxic T lymphocytes cells, NK cells and phagocyte.

IFN-γ产生于抗原激活的T细胞和NK细胞，它扮演了涉及到抗病毒和抗肿瘤功能的免疫调节角色和效应因子功能，包括诱导CTL细胞、NK细胞和吞噬细胞依赖的活性的激活。

Methods Core b1od mononuclear cells were isolated by Ficoll, Cd34(superscript +) cells were purified from mononuclear cells by MACS and then cultured in a complete medium containing rhIL-4, GM-CSF, rhSCF, rhFlt-3 Ligand, TGF-β1 and TNF-α. After 14d of incubation, the morphology of the cells was observed, the phonotype was examined and the IL-12 level in the medium was measured.

Ficoll分离脐血单个核细胞，免疫微磁珠法分离纯化CD34细胞，以rhIL-4、GM-CSF、rhSCF、rhFlt-3 Ligand、TGF-β1，及TNF-α体外诱导培养14d，进行形态学观察、表型检测及上清液IL-12测定。

Results:After 5 days of disposal of 8-MOP combined with ATRA or alone on Mc3 cells,the numbers of Mc3 cells are decreased,the division cell disappears,the rugosity of cells surface is changed into the flat shape,the extension dimension of Mc3 cells enlarge obviously.

结果：Mc3细胞经8-MOP与ATRA单独及联合应用处理5天以后，细胞数量减少，分裂细胞消失，细胞表面由折皱状改变为平铺状，细胞伸展面积明显变大。

EGF was mainly located in the cytoplasm and extracellular matrix of intestinal villus cells, endothelial cells and tunica serosa epithelial cells, while EGFR chiefly distributed in the cellular membrane of these cells.

其中 EGF主要存在于小肠黏膜上皮细胞、黏膜下层的血管内皮细胞内和浆膜上皮细胞的胞浆和胞外基质中，EGFR则分布于这些细胞的细胞膜上。

Initial adherenced cells mostly are round or short fusiform. Division growth of adherenced cells was shown 72 hours after fully changing suspension(.2)Flow cytometry analysis of the adherenced MSCs indicated good homogenicity of passage cells. More than 95% CD34- and CD44+ demonstrated the cells we get were purified MSCs.

2对贴壁生长的MSCs进行流式细胞仪检测，提示传代的细胞均一性较好，表面抗原CD34表达阴性，CD44表达阳性的细胞均在95%以上，说明得到的是比较纯化的MSCs。

Although translator has turned from being a crystal ball by which the original culture can unrestrainedly penetrate to another crystal ball by which the target culture can freely traverse, the translator's personal embodiment, in the process of cognitive act, are still absent in translation studies. Translators are still subjects without body or simply disembodied subjects.

译者虽然由原语文化可以自由穿透的玻璃球变成了译语文化可以自由穿越的玻璃球，但译者认知过程中的个体体验在翻译研究中依然缺席，译者依然仅仅是一个没有躯体体验的主体。

Chillingly, he claimed our technology is 'not nearly as sophisticated' as theirs and "had they been hostile", he warned 'we would be been gone by now'.

令人毛骨悚然的，他声称我们的技术是'并不那么复杂，像他们一样，和"如果他们敌意"，他警告说，'我们将现在已经过去了。