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To dissect single cells promising to culture from tissues, we researched the time of hydrolyzation of the tissues by tryposin, the result showed that the optimum time of hydrolyzation of the tissues was 5~10min when pretreated with 0.25% tryposin under the optimum conditions of pH8.0 at 37 C.We cultured four tissues: embryo at mediat-anaphase, head of high-age nymphae, spermary and ovary of adult.

组织块分离出来后，为了能获得单个的、有培养前景的细胞，本实验又研究了低浓度的胰蛋白酶在其最适温度和最适pH条件下，获得分离程度适宜的组织块的不同酶解时间，结果0.25％的胰蛋白酶在37℃、pH8.0的条件下酶解组织块的最适时间是5～10min。

Thirty patients with acute promyelocytic leukemia were observed between September 1977 and October 1985. Bleeding diathesis with petechiae or ecchymosis (80%) was the most common presenting symptom. Seventeen of 24 patients with pretreated coagulation studies were diagnosed to have disseminated intravascular coagulation. Six out of 11 patients died from intracranial hemorrhage after a mean of 2.5 days of hospitalization without any treatment. DIC was responsible for death in most cases. Induction therapy with anthracycline and cytosine arabinoside combined with prophylactic heparin infusion was carried out in the remaining 19 patients. Two patients were incompletely treated. Eight out of 17 patients achieved complete remission after a mean of 63 days following chemotherapy. The remission rate was 47%.

自1977年9月至1985年10月，观察30位急性前骨髓球性白血病病帣，临床上诊断时初发症状，主要为出血现象，尤其以瘀斑出血最多，占80%。24位病人作治疗前凝血机能检查，17位出现播散性血管内凝固症候群。11位病人没有接受治疗，其中6位经诊断后平均2.5天内死於脑内出血，凝血机能检验显示5位有播散性血管内凝固症候群。19位病人接受anthracycline与ara-C并用预防性肝素静脉滴注作缓解诱导治疗，2位不完全治疗，其余17位中有8位经过平均63天之化学治疗得到完全缓解，缓解率47%。

Calcium montmorillonite was prepared by purifying LeXi bentonite with wet method two times. After purification, sodium modification of Ca-M was studied. A series of Fe/Cr-pillared montmorillonites were prepared with conventional method using sodium montmorillonite as experimental materials, and synthesis condition of Fe/Cr-PILM was studied and optimized. In order to shorten the preparation cycle of PILM by overleaping the step of sodium modification of Ca-M, the pretreatment technique for Ca-M has been pointed out for the first time. A series of PILMs were prepared with hydrothermal method using pretreated Ca-M as starting materials, which show that pretreatment of Ca-M is feasible.

然后，本文以莱西膨润土为原料，用二次湿法提纯了-2μm的钙基蒙脱石，对其进行了钠化改型研究；以改型后的钠基蒙脱石为原料，新型的铁、铬复合离子为柱化剂，研究并优化了常规离子交换法制备PILM的工艺；为了简化PILM的制备工艺，我们首次提出了蒙脱石的预处理工艺，希望通过预处理后可以省去蒙脱石的钠化工艺，以Ca-M为原料直接合成PILM。

Thermally denatured DNA was preconcentrated at pretreated GCE by its adsorption in open circuit for 5min or at the potential of+0.3V for 90s, and produced two well-defined oxidation peaks of guanine and adenine residues at+0.80V and+1. 11V in pH 5. 0 phospoate buffer, respectively, but the native DNA did not produce any peaks at the same conditions.

在pH 5.0的磷酸盐缓冲液中，于+0.3V富集90秒或在开路条件下吸附富集5分钟，热变性DNA可以吸附富集在预处理玻碳电极表面，阳极扫描时，鸟嘌呤和腺嘌呤残基产生两个很好的氧化峰，峰电位分别为+0.80V和+1.11V，而在同样条件下，天然DNA却几乎不出现峰。

3Cr2W8V hot work die steel was pretreated by rapid precooling annealing process instead of traditional annealing process.

采用快速预冷退火工艺取代传统退火工艺，对3Cr2W 8V热作模具钢进行了预处理。

Air is pretreated in photocatalyst prefilter module. Big particular matter is filtered in this stage.

空气在冷触媒初效过滤模块进行预处理，去除空气中较大的颗粒物。

NO+NH3 temperature-programmed reaction runs over reduced catalyst samples pretreated with various oxidizing species showed that both NO2 and HNO3 were able to reoxidize the V catalyst at much lower temperature than gaseous O2: furthermore, they significantly enhanced the NO + NH3 reactivity below 250 C via the buildup of adsorbed nitrates, which act as a surface pool of oxidizing agents but are decomposed above that temperature.

不+NH3 温度-规划反应奔跑结束减少催化剂样品预先处理与各种不同的氧种表示两者的没有 2 和 HNO3 能够到再氧化 V 催化剂在非常低的温度超过气体的 O2：此外，他们重要地可提高的那不+ NH3 反应度在下面 250？C 经由组织吸附硝酸盐，哪一个行为当做一表面池氧代理人但是被分解上方哪一温度。

Resinic artificial teeth should be deposited with 50nm nanometer amorphous diamond film after pretreated with polarization.

纳米非晶金刚石薄膜能够提高树脂人工牙的耐磨性能，处理的方法应选择极化法，薄膜的厚度控制在50nm为最佳。

Methods The resinic artificial teeth were deposited with 50nm or 100nm nanometer amorphous diamond film after pretreated with ultraviolet radiation or polarization.The effect of different methods of pretreatment and thickness of nanometer amorphous diamond films on the wearing resistance of resinic artificial teeth was evaluated.

分别用极化和紫外光照射的方法对人工牙进行预处理后，在其表面分别镀制50nm和100nm的纳米非晶金刚石薄膜，通过对其耐磨性能的测试，评价不同预处理方法和不同厚度薄膜对提高人工牙表面耐磨性能的影响。

Results Resinic artificial teeth deposited with 50nm amorphous diamond film after pretreated with polarization are most close to the natural enamel. Conclusion Nanometer amorphous diamond film improves significantly the wearing resistance of resinic artificial teeth.

结果：纳米非晶金刚石薄膜能显著提高树脂人工牙耐磨性能；两种预处理方法中，极化法实验组的耐磨性能明显优于紫外光照法实验组；极化处理后镀膜50nm的人工牙的耐磨性能最接近于天然牙釉质。

Do you know, i need you to come back

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Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。