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Methods: During the April 2003 to December 2004, from the patients with different position colorectal cancer beyond Dukes C stage who were performed laparoscopic radical operation random draw 20 patients as experiment group, and from patients with different position colorectal cancer beyond Dukes C patients who were performed open radical operation 20 patients took randomly as contrast group. We draw thepatients blood about 5 ml from antecubital vein at the time before and after the operation about 30 minutes respectively, preserved the blood in deep hypothermia circumstance,cytrokeratin-20(CK-20) as the micrometastasis maker, using the RT-PCR technique,G3PDH as the intra-contrast gene, quantitatively detect the amounts of CK-20 in the blood with the gelatum imagery system and the computer analyzing software on the gelatum imagery system ,in order to compare the influence of the laparoscopic and the open operation on the micrometastasis.

在我院2003年4月—2004年12月间不同部位的临床分期在Dukes C期之内的接受了腹腔镜结直肠癌根治术结直肠癌患者中随机抽取20例作为试验组，另外在同期的接受开腹手术患者中随机抽取20例作为对照组，分别抽取术前30min和术后30min的肘前静脉血5ml，集中低温保存，以细胞角蛋白(cytekeratin-20)CK-20为微转移的标志物，应用RT-PCR技术，以G3PDH为内对照基因，应用凝胶成像系统及其计算机凝胶成像系统分析软件来定量检测手术前、后患者外周静脉血中CK-20含量，比较腹腔镜和开腹的手术方式对肿瘤微转移的影响。

In this study, viabilities of hair follicles preserved at different temperatures and storage media for various periods were evaluated and compared by cell cultures and athymic mice transplantation.

本研究以细胞培养和裸鼠移植的方法比较了毛囊保存于室温(26℃)和低温(0和4℃)状况下，以及在不同保存液中不同时间后的活性。

This edition has repaired before edition some small questions, increased the dynamic alignment time delay, turns on, closes the microphone and the sound, preserved and the autoloading talk address tabulates, acts the server to tabulate, uses completely or partial this machine address opening machine ,Delphi

本版本修复了以前版本的一些小问题，增加了动态调整延时，打开、关闭话筒和声音，保存和自动加载通话地址列表，代理服务器列表，使用全部或部分本机地址开机

The preservation of axenic strain of D. salina was investigated with different ways. The results showed the algae could be preserved in liquid medium, plate, and slant.

采用不同方法对盐藻进行保种研究，结果表明盐藻适宜于液体、平板和斜面保种。

RESULTS: Myocardial cells of the adult rats were preserved with ultrahypothermia liquid nitrogen storage and a sufficient number of bacilliform myocardial cells which met the quality standard were separated.

结果：采用成年大鼠心室肌细胞超低温液氮保存分离法，成功地分离出足够数量符合质量的杆状心肌细胞。

Traditional phenotype classification,chemotaxonology and other ordinary bacteria taxonology were used to study the antagonistic bacterium preserved by the agricultural antibiotic laboratory of Chengdu Institute of Biology, Chinese Academy of Sciences.Its morphological,cultural physiological,biochemical characteristics and chemotaxonomy were studied.The antagonistic bacterium is bacilliform,and its cell diameter is less than 1 micron;it is Gram positive;it.can form spore.

采用传统的表型分类、化学分类等一般细菌常用鉴定方法，对中科院成都生物所农抗组实验室保存的一株小麦赤霉菌拮抗菌株进行形态特征、培养特征、生理生化特征等水平的鉴定，该细菌的细胞呈杆状，细胞直径小于1微米，革兰氏染色阳性，能形成芽孢，属于芽孢杆菌属。

The hemolytic ability and bacteriolytic ability of serum improved evidently over the control group,preserved at a high level until 96 h after injection.

血清的溶血能力、溶菌能力与对照组相比有明显提高，一直维持到96 h仍具较高水平。

The brachiopoda fossils of Cambrian of the Kaili Formation in Guizhou are well preserved and in a large quantity.There are 9 genuses.10 specieses and 3 uncertain specieses including 1 new specieses after a tentative studing.

贵州寒武纪凯里组腕足动物化石保存较好，数量较多，初步研究有有9个属10个种3个未定种，其中1个新种。

Twenty intact and well preserved cadaveric femoral samples were provided by the Department of Anatomy, Hebei North University.

实验对象：20例保存完好的人尸体股骨标本由河北北方学院解剖学教研室提供。

The rule of fasting, somewhat less severe among the Calced Carmelites, is preserved everywhere, although the church has in many respects mitigated her legislation in this matter.

法治禁食，少一些严厉当中calced carmelites ，是保存到处，虽然教会在许多方面减轻了她的法例，在这件事。

And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47：5 法老对约瑟说，你父亲和你弟兄们到你这里来了。

Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.

提出了用测地线方程、曲面上两点间短程线来计算膜结构曲面测地线的方法，同时，采用测地线间垂线的中点连线和用无约束极值法进行空间条状曲面近似展开的分析。