查询词典 precooled

The acetone precipitation group: bile sample was washed with 4 times volume of precooled acetone, dried by vacuum freeze dryer and dissolved with lysis buffer.

在胆汁标本中加入4倍体积的预冷丙酮，用预冷的丙酮洗涤沉淀，然后用真空冻干机抽干，用裂解液溶解沉淀过夜。

The trichloracetic acid/acetone precipitation group: 4 times volume of mixture of trichloracetic acid/acetone was added in the bile sample, washed with 4 times volume of precooled acetone, dried by vacuum freeze dryer and dissolved with lysis buffer.

在胆汁标本中加入4倍体积的预冷三氯乙酸/丙酮混合液，用预冷的丙酮洗涤沉淀，然后用真空冻干机抽干，用裂解液溶解沉淀过夜。

The dissertation presents somecontents about the vacuum precooling as follows: 1. Studies on the evaporation cooling principles of fruit and vegetable There are different optimal storing conditions for different vegetables and fruits.And the vegetable and fruit precooled by vacuum must be fit with the storingcondition. A low temperature will result in the frozen scald, and a high temperaturewill not meet the precooling objective.

本文开展了以下几个主要方面内容的研究：1、果蔬蒸发冷却规律的研究不同果蔬都有各自不同的最佳储藏条件，因此，真空冷却后的果蔬必须满足储藏要求，温度过低易引起果蔬冻伤，过高则达不到预冷的目的；湿度过低易引起干耗，减轻重量；过高则易引起腐烂变质。

An improved method that facilitates the extraction of PCR-compatible faecal DNA from giant panda\'s faeces is described. The method involves a novel preprocessing step in DNA extraction. The faeces is washed two or three times with precooled acetone, which removes numerous potential PCR inhibitors, and then digested with proteinase K. The DNA is purified with phenol/chloroform.

在粪便DNA的提取过程中采用一个新的预处理方法，将粪便用预冷的丙酮洗2～3次，除去粪便中含有的大量PCR抑制物，然后蛋白酶K裂解、酚-氯仿抽提，能提取到纯度很高的DNA供PCR扩增。

B:Oocytes and early embryos were permeated in 0.5%Triton X-100 for 10 minutes,then fixed in 4% paraformaldehyde/PBS.Method C:Oocytes and early embryos were fixed in 1%PFA while permeated in 0.2%Triton X-100 at the same time for 1 hour.Method D: Oocytes and early embryos were fixed in precooled methanol(-20℃) for 15 min,then permeated in 0.5%Triton X-100 for 10 minutes.

方案A：先用4%多聚甲醛固定1h，再用0.5%Triton X-100穿透10min；方案B：先用0.5%Triton X-100穿透10min，再用4%PFA固定1h；方案C：用1%PFA+0.2%Triton X-100同时固定与穿透1h；方案D：用-20℃预冷甲醇固定15min，再用0.5%Triton X-100孵育10min。

The concept of equivalent rotationally rigidity is offered and the formula of rotationally rigidity is obtained.

主要做了如下几个方面的工作：对伸臂位于顶部的单层框架—筒体模型进行分析，提出了等效转动约束的概念和转动约束刚度的表达式。

Male cats normally do not need aftercare with the exception of the night after the anesthetic.

男猫通常不需要善后除了晚上的麻醉。