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We amied the BCP sequence and the preS2 gene sequence of HBV separately as the target region, according to HBV DNA sequence of Chinese strain, synthesized the set of specific primers, amplified the sequence by PCR method from the serum of patients with CHI, verified by restriction analysis, subcloned into pGEM Teasy vectors, employed polyacrylamide gel electrophoresis to display the deletion mutation and selected the clones with differential length to be sequenced.

本研究应用PCR技术及其他分子生物学技术,分别以HBV转录调控序列BCP以及表面蛋白编码序列pre S2为靶基因,以中国株HBV基因序列为依据,设计特异性多聚酶链反应引物,分别自43例、51例慢性HBV感染患者血清中扩增出目的片段,构建质粒Teasy-BCP和Teasy-pre S2,酶切鉴定后,采用聚丙烯酰胺凝胶电泳技术展示缺失突变,再经DNA测序以了解在慢性乙型肝炎患者体内的病毒变异情况。

Methods Twenty-two patients with mild pre-eclampsia, 28 with severe pre-eclampsia, 10 with eclampsia as well as 30 healthy pregnant women and 20 healthy unpregnant women were determined for GP Ⅱb/Ⅲa by flow cytometry and for urea nigrogen, creatinine as well as uric acid by full-automatic biochemical analyzer, and the results were compared.

分别采用流式细胞术和全自动生化分析仪,检测子痫前期及子痫患者、正常孕晚期、正常未孕妇女血中GPⅡb/Ⅲa水平和肾功能相关指标(BUN、CREA、UA)。

Compare the lung injury extent and the important physiological parameters to determine the optimal condition for rabbit ventilator induced lung injury, Choosing another 24 new Zealand rabbit, randomly divided into 4 groups:(1)conventional ventilation+PEEP group;(2)ventilator induced lung injury group: employ the experimental condition determined by the first part experiment;(3)VILI+HO-1 inducer hemin group:pre-treated with HO-1 inducer, the ventilation method is the same with VILI group;(4)VILI+HO-1 inhibitor Zn-PP group(Zn-PP group):pre-treated with HO-1 inhibitor, the ventilation condition is the same with VILI group.

第二部分24只家兔随机分为4组:(1)常规潮气量+PEEP组;(2)呼吸机所致肺损伤组:使用实验第一部分确定的最适条件;(3)呼吸机所致肺损伤+HO-1诱导剂干预组:预先给予氯铁血红素,通气条件同VILI组;(4)呼吸机所致肺损伤+HO-1阻断剂干预组:预先给予锌原卟啉,通气条件同VILI组。

The occurrence of a pre-endotherm is observed when a properly annealed HQG undergoes a calorimetric upscan. The pre-endotherm is followed by an exotherm due to the release of the excess energy stored during hyperquenching, and subsequently by the endothermic glass transition.

如果对一个适当退火的急冷玻璃升温差热扫描,将会先后出现预吸热峰,释放急冷中储存在玻璃中能量的放热峰,以及玻璃转变吸热峰。

The at least one data retention indicator device (50) comprises a first magnetic element (51) and a second magnetic element (52) each having a pre-set magnetisation direction, the pre-set magnetisation direction of the first and second magnetic elements (51, 52) being different from each other.

该至少一个数据保持指示器器件(50)包括第一磁性元件(51)和第二磁性元件(52),这两个磁性元件中每个都有预置的磁化方向,所述第一和第二磁性元件(51,52)的预置磁化方向互不相同。

Based on studying Gadamer"s Truth and Method, this paper centers on unearthing Gadamer"s pre-seeing theory and effective history theory in his Hermeneutics, and points out that pre-understanding is indispenasable to understanding, succeeds to individual and history and cants be rejected by individuals.

重点分析前见,指出:前见是理解的必要条件,是个人与历史文化的存在上的承接,是个人无法拒绝的。

In order to realize the pre-mixed water jet flow ultra fine coal comminuting devices automatic control, raises the fine coal of smashing efficiency and the product quality, designed the pre-mixed water jet ultra-fine coal comminuting configuration monitoring system.

摘 要:为实现前混合水射流超细煤粒粉碎装置的自动控制,提高煤粒粉碎效率和产品质量,设计了前混合水射流超细煤粒粉碎装置的组态监控系统。

The thick evaporates in the middle of the fourth member of the Shahejie Formation compartmentalizes the overpressure system in the Paleogene into a pre and a postsalt compartment, resulting in a pre and a postsalt gas system correspondingly.

由于沙四段中部大套膏盐岩的分割,古近系深层超压体系具有盐上、盐下两个次一级超压&封存箱&系统,相应形成盐上、盐下两套天然气成藏系统。

The main Product sales have: 200A-3 Oil Press, 202A-3 pre-pressing machines, 24, 240 type, 204-type pre-pressing machine, 1.5x5 steaming pan, 1.8x5 steaming pan, 2.1x5 steaming pan 50,60 x125 , 100x150 on the roller, hydraulic rolling machine meal, dryers, vibrating fluid bed dryer, frame-style crude oil filter machine, centrifuge, deodorization tank, detreated cans, refinery tanks, off soap mixer machine than the allocation, heating cans, dryer, 28-36 decorticating machine, round screen, vibrating screen, flat screen, crusher, to stone machine, steam offline, deceleration machine, condenser, Li-cutter apartments, flat cutter cage, hoist, etc.,15-150 tons of oil extraction plant, salad oil workshop, hydrogenated oils workshop, to deal with workshop, scouring plant derivative, 1-six tons of oil steam boiler, generator sets.

主要销售产品有:200A-3榨油机、202A-3预榨机、24型、240型、204型预榨机、1.5x5蒸炒锅、1.8x5蒸炒锅、2.1x5蒸炒锅50、60x125、100x150对辊、液压轧粕机、烘干机、振动流化床干燥机、板框式毛油过滤机、离心机、脱臭罐、脱色罐、精炼罐、脱皂机比配混合机、加热罐、脱水机、28-36剥壳机、圆筛、振动筛、平筛、破碎机、去石机、蒸脱机、减速机、冷凝器、立绞笼、平绞笼、提升机等,15-150吨浸出油车间、色拉油车间、氢化油车间、予处理车间、精练车间、1-6吨导油蒸汽锅炉、发电机组。

Scientific and reasonable grid planning has a direct relationship with safe and reliable operation of the grid, power supply reliability for the user, and operational flexibility of the system. The 220 kV substation power supply capability evaluation based on analysis of the supporting ability of 35 kV girds was applied to the grid pre-planning management information system, which enables pre-evaluation for the gird in planning to search for defects and make improvement.

电网的科学合理规划将对今后电网的安全稳定运行、保证用户供电的可靠性和系统运行的灵活性带来直接影响,在电网规划前期管理信息系统这一平台上,运用基于35kV网架支援能力分析的220kV变电站供电能力评估的方法,即可对规划中的电网进行预先评估,通过评估的结论,查找电网存在的不足以求持续改进。

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相关中文对照歌词
Quand J'étais Chez Mon Père
Pre-You
Parler À Mon Père
Pre-Road Downs
Pre-Road Downs
Pre
Mon Père (Father - French Version)
Pre-Teen Violence
Pre-Medicated Murder
推荐网络例句

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm~150rpm时取得最大值,此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世,形体几无变化。

When I was in school, the rabbi explained everythingin the Bible two different ways.

当我上学的时候,老师解释《圣经》用两种不同的方法。