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In this study, we found that the positive immunostaining for αSMA was increased in group DN from week 4 onward, mainly located in dilated tubuli, which was more evident at week 8. The double staining for CTGF and p27kip1 showed that the increased positive staining for both antibodies was more evident in tubuli and some glomeruli.

正常大鼠肾脏可以有少量细胞P27kip1染色阳性，本研究发现，在DN模型组，第1周起肾小球和肾小管P27kip1染色阳性细胞即显著增加（P＜0.01），且随着病程进展，P27kip1染色阳性细胞比例显著增加。

The relationships of VEGF with the indices of renal damage, including renal/body weight, urinary protein excretion, glomerular volume and glomerular area, were analyzed. Results The expression of VEGF mRNA in diabetic kidney was significantly up-regulated after operation from 2 weeks to 24 weeks with the peak level at 20weeks, when compared with control at the same time- points. The positive results of VEGF staining in diabetic glomeruli was increasingly observed after operation from 2 weeks to 24 weeks, with the peak at 20 weeks. The positive results of VEGF staining in diabetic tubuli was increasingly seen from 2 weeks to 24 weeks, with the peak at 8 weeks.

结果 VEGF mRNA在糖尿病模型组大鼠肾脏中持续高表达，各时点与对照组相比差异均有统计学意义(P.05)；糖尿病组2~24周肾小球VEGF蛋白表达强于对照组(P.05)，24周时有所减弱，但仍强于对照组；糖尿病组各时点肾小管VEGF蛋白表达均强于对照组(p.05)， 8周后表达明显增强；VEGF表达变化与肾质量／体质量(r=0.518， P＝0.001)、尿蛋白(r=0.409， p=0.001)、肾小球面积(r=0.499， P-0.000)和体积(r=0.499， P-0.000)呈正相关。

The couples with uncompatible ABO System the positive percentage was 54.8%(160 cases), and 27.4%(80 cases); The couples with compatible ABO system the positive percentage was 67.6%(23 cases) and 50%(17 cases).

夫妇ABO血型系统不合的阳性率分别为160例(54.8%)，80例(27.4%)；夫妇ABO系统血型相合的阳性率分别为23例(67.6%)，17例(50%)。

For packaging attributes, small size parcel with 2 kg capacity have more positive evaluation than the medium size parcel (2-4 kg). Parcels with labeling that indicates unhusked grains are stored in low temperature also received positive evaluation.

包装特徵方面，容量在2公斤以下的小包装较中包装（2-4公斤）能获得正面评价，包装标示上标示稻谷原料低温冷藏获得正面评价。

On the packaging side, small size parcel with 2kg capacity have more positive evaluation than the medium size parcel (2-4kg), and parcels with labeling that indicates unhusked grains are stored in low temperature also received positive evaluation.

三包装特徵方面，容量在2公斤以下的小包装较中包装（2-4公斤）能获得正面评价，包装标示上标示稻谷原料低温冷藏获得正面评价。

ANP and CX43 began to express at 2nd week after induction and increased gradually,about 60% of the resulting myogenic cells were positive at 4th week after induction ,they were negative for uninduced cells.hMSCs'surface antigen profiles obtained by Flow Cytometry were negative for CD31\CD34\CD45 before and after induction,but CD90 expressed higher after induction while was weak positive before induction(P.05). Apotosis index was correlated with the cultural time after induction,The apoptosis rate of induced hMSCs was remarkably higher than control group(P.01),and the variation between groups was notable(P.05),the cell cycle analysis showed that the percentages of G_0/G_1phases were reduced significantly after induction. The expresstion levels ofβ-MHC and CTNT mRNA were undetectable before induction,began to increase at 1st、4th week after induction,reached the peak at 6th week and decreased after that,the expression of Bcl-2 and Bax mRNA varied regularly after treated with 5-azacytidine. hMSCs'resting membrane potential、range and rate of depolarization were heightened gradually after being induced.

结果：hMSCs诱导前为纺锤形，诱导后第2天部分细胞即开始发生形变，呈球形或短棒状，1周后胞浆中颗粒增多，约20～30%细胞边缘呈毛刷样变化；hMSCs表面抗原CD31、CD34、CD45在诱导前后差异无统计学意义，CD90未诱导时表达呈弱阳性，诱导后明显增高(P.05)；ANP和CX43在诱导前无表达，诱导后第2周开始表达且表达随时间逐渐增强，但CX43在诱导后第5周表达量开始降低。hMSCs诱导后凋亡指数随诱导后培养时间增加，低浓度诱导组低于标准浓度诱导组，组间差异有统计学意义(P.05)，G_0/G_1期细胞比例诱导后较对照组显著减少(P.05)；β-MHC和CTNT基因分别在诱导后第1周和第4周时表达开始增强，在第6周时均达到高峰，第8周时表达开始衰减，Bcl-2、Bax基因表达呈时间依赖性变化，hMSCs经诱导后随心肌样细胞特征的表达膜静息电位、去极化幅值和去极化速率逐渐增高。

Our results shown: Compared with those of the unradiated rats, the number of S-100 protein immunoreactive positive glial cells in the brains of the irradiated rats increased gradually. More S-100-positive glial cells with multiple long processes and hypertrophic cell bodies were also observed in these areas.

在此基础上，又做了同一照射剂量（20Gy）照射大鼠半脑后不同存活期间（id，7d；14d，Zld，28d）大鼠全脑S－100动态变化的观察，发现其规律如下：在上述时间点大鼠脑内各部位卜刁m蛋白免疫反应细胞数量进行性增加，伴有 S－100免疫阳性细胞的胞体逐渐增大和交起增多，与正常对照组具有明显的差异。

Results There were no sinificant difference in incidence of aneuploid and highs synithesis phase fraction, the size and distribution of AgNOR between unrecurrent and recurrent tumors, and there were sinificant difference between benign and malignant tumors, and there were singnificant differenct in the number and shape of AgNOR between the groups, and a linear positive correlation was observed between that obvious positive correlation was observed between highs SPF and number of AgNOR.

结果 异倍体率，高S期细胞比率率，AgNOR大小和分布在无复发与复发组间无显著差异，而良性与恶性肿瘤间有显著差异；AgNOR数目和形态各组间有显著差异；高SPF率与AgNOR均能反映其细胞增殖活性。

Genomic DNA of positive transformed plants; 6, 7: Genomic DNA of negative transformed plants; 9: genomic DNA of untransformed plants; 10: Positive control of 3G1MHpCAMBIA2301; 11: NTC; 12: DL 2000 marker.

2.2 转基因拟南芥的筛选选择培育后获得转基因拟南芥植株。从其叶片中提取微量DNA， PCR鉴定，可扩增出750 bp左右片段（图3），表明3G1MHpCAMBIA2301基因已整合到拟南芥染色体中。

Meanwhile, positive human sera of HCV challenged vaccina ted monkeys after 6 weeks immunized, ALT value rose transiently in monkeys vacci nated by PCX. It was positive in monitoring RNA of HCV using RT-PCR in monkey s era.

同时，在免疫后6周用人HCV阳性血清攻击猴子，免疫PCX的猴子出现一过性ALT升高，在攻击后三周内用 RT－PCR检测到猴血清内HCV的RNA阳性。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。