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Immunohistochemical assay were performed with HGV NS5 McAb. Results One of four samples showed positive staining, some of which located in cytoplasm and some of which in cytonucleus and cytomembrane. The number of positive staining cells are not more. More of them disrtibuted scatteredly, some of them located.

结果 1例呈阳性染色,光镜观察显示:特异染色大部分定位于肝细胞胞浆中,部分有核着色和膜型染色,呈黄色或棕黄色颗粒状,阳性细胞数量少,大多散在分布,部分呈灶性分布;阳性细胞周围可见较多淋巴细胞浸润。

These 375 patients had a median age of 50.57±10.46(range,19-72) with 87.4%HBsAg positive and 4.3%anti-HCV antibody positive;The apparent peak incidence age was 40~60 years old,and the ratio of male to female was 10.7:1;The 3 and 5 year postoperational survival rate were 52%and 38%;The tumour numbers(p=0.000),tumor size(p=0.025),histological pattern (p=0.000),nuclear features(p=0.000),differentiation(p=0.001) and vascular invasion(p=0.000) were significantly correlated with prognosis.The postoperational survival time of thin trabeculae pattern,compact pattern and pseudoglandular pattern were significantly longer than that of thick trabeculae, scirrhous pattern,and solid patternp<0.009the postoperational survival time of 1 and 2 grade based on nuclear features were significantly longer than that of 3 and 4 grades(p=0.000The small cell variant,osteoclast-like giant cell variant, and spindle cell variant were mainly composed of thick trabeculae pattern and solid pattern,which were significantly different from that of clear cell variant.

结果1。本组资料显示肝细胞癌发病年龄19~72岁,平均50.57±10.46岁,发病高峰年龄40~60岁,男女比例为10.7:1;HBsAg87.4%,anti-HCV抗体4.3%;术后3年生存率为52%,5年生存率为38%;肿瘤数目(p=0.000)、肿瘤大小(p=0.025)、组织学结构类型(p=0.000)、核分级(p=0.000)、分化程度(p=0.001)及血管浸润均(p=0.000)与预后明显相关;其中组织学结构类型中细梁状型生存时间与致密梁状型和腺样型无明显区别(p>0.05)而明显高于粗梁状型、实性型和硬化型(p≤0.009),硬化型生存时间与实性型之间无明显区(p>0.05)而明显低于其余各型p≤0.006核分级1级与2级生存时间无明显区别(p>0.05,核分级3级与4级生存时间无明显区别(p>0.05),而核分级3级生存时间明显低于2p=0.000小细胞型、巨细胞型和梭形细胞型主要由实性型和粗梁状型组织学结构类型构成,明显不同于透明细胞型(主要由细梁状型和粗梁状型构成(p≤1.006)。2。

For , it can be shown that , and In chapter 2, using some well-known results, several inequalities on eigenvalues of the generalized Schur complement of BAB* where A is positive semidefinite are obtained:(1) If ,,, and l is an positive integer satisfying

第二章对形如BAB~*的矩阵乘积,介绍了几个关于其Schur补的特征值不等式,并在已有结果的基础上,利用定理1.1.1,得到了其广义Schur补特征值的诸多不等式。

Methods: thirty nine full term placentae of hbsag sero positive pregnant women were collected, in which 23 were detected as hbsag positive by sabc immunohistochemistry staining.

收集血清学hbsag阳性孕妇足月胎盘组织39例,经sabc免疫组化方法在其中23例中检测到hbsag,随机选取7例采用免疫荧光双标记方法结合激光共聚焦技术检测hbsag/annexin v。

For the cases of CHD,the level of plasma endothelin-1 in these of HPIgG seropositivity is higher than that of HP seronegativity(P.05).(4) No correlation between chronic HP infection and essential hypertension,diabetes mellitus,tobacco use,age,gender,vocation,living condition ,alcohol consumption and family history was found.(5) For the cases of positive for Carbon-14 urea breath test in CHD patients, the level of plasma GLU is higher than that of negativity for Carbon-14 urea breath test (P.05).The rate of positive for Carbon-14 urea breath test in CHD patients with diabetes mellitus is significantly higher than that of CHD patients without diabetes mellitus.

结果(1)冠心病组血清HPIgG阳性率明显高于非冠心病组(45.54%vs 28.30%,P<0.05);(2)冠心病组C-14呼气试验阳性率明显高于非冠心病组(54.46%vs 30.19%,P<0.01);(3)冠心病组慢性HP感染者血浆ET-1水平明显高于非HP感染者(P<0.05);(4)慢性HP感染与血脂、高血压史、糖尿病史、吸烟史、年龄、性别、职业、生活环境、饮酒史以及冠心病家族史无关;(5)现症HP感染与糖尿病及空腹血糖升高有关(P<0.05);与血脂、高血压史、吸烟史、年龄、性别、职业、生活环境、饮酒史以及冠心病家族史无关。

Results ①Five days after abdominal application of Xiaoji power(0.1 g per mouce),the medicine showed a very significant effect in enhancing gastrointestinal propulsive function.② Xiaoji powder not only had relaxing effect on the normal smooth muscle in vitro,but also had a strong spasmolytic effect on acetylcholine,green bariuminduced spasm of intestinal smooth muscle.The experiment was based on 10 μg atropine sulfate as a positive control to carry out control experiments.It showed the graphics of atropine sulfate in spasmolytic role.③Xiaoji powder navel paste therapy played a positive role in adjusting the gastrointestinal electric disorder caused by deficiency of spleen Qi,and could restore it to its normal state.

结果 (1)以0.1 g/只消积散给予小鼠腹部用药5 d,即显示非常显著的增强胃肠推进功能作用;(2)消积散不仅对正常离体平滑肌有舒张作用,同时对由于乙酰胆碱、氯化钡引起的肠平滑肌痉挛也有强的解痉作用,该实验是以硫酸阿托品10 μg作为阳性对照药进行对照实验,显示了硫酸阿托品的解痉作用图形;(3)消积散敷脐对脾虚引起的胃肠电紊乱有明显的调节作用,使脾虚大鼠的胃肠电基本恢复到正常状态。

It was seen that the main microflora causing the spoilage of fresh mutton meat consisted of 3 gram-positive bacilli with spore (about 30% of the micro flora), 1 gram-positive coccus (about 2% of the whole microflora), 3 gram-negative spirilla (about 63% of the whole microflora), and 1 gram-negative bacillus (about 5% of the whole microflora). Potassium sorbate, EDTA and nisin were used as preservatives to inhibit the growth of the main strains representative for the spoilage of fresh lamb meat.

对屠宰后羊肉的微生物区系进行了调查,成功地分离出引起冷却羊肉腐败的主要革兰氏阳性菌和阴性菌8种,其中含芽孢的革兰氏阳性杆菌有3种,占整个微生物区系的30%;革兰氏阳性球菌1种,占整个区系的2%;革兰氏阴性螺旋杆菌3种,占整个区系的63%;革兰氏阴性杆菌1种,占整个区系的5%。

There were no significant differences for the firing rates in the site of contralateral TNC neurons among during pre-CSD,CSD,and post-CSD (P>0.05).For flunarizine group,the firing rates in the site of ipsilateral TNC neurons during pre-CSD were higher as compared with during CSD(P<0.05).2.1 There were statistical differences on palasma levels of CGRP and SP among the three groups(P<0.05).The levels of CGRP and SP in CSD group were higher than control group(P<0.05).No significant differences on the levels of CGRP and SP in ipsilateral trigeminal ganglia were found among the three groups(P>0.05).2 The number of neurons with positive CGRP and SP immunoreactivity was statistically different in right-sided trigeminal ganglia among the three groups (P<0.05).The number in fight-sided trigeminal ganglia in CSD group was higher as compared with control group(P<0.05).The number in right-sided trigeminal ganglia was statistically higher than that in left-sided trigeminal ganglion in CSD group(P<0.05).3.1 Altered ReHo in ipsilateral pons and other brain regions response to pain such as basal nuclei,thalamus,cingulated gyms and prefrontal cortex was detected during the acute spontaneous attack as compared with during headache remission(P<0.05,corrected by Monte Carlo simulation). 2 Positive functional connectivity was detected between ipsilateral pons and other brain regions related to pain within pain state and within non-pain state (P<0.05,corrected by false discovery rate,FDR).Increased functional correlation between ipsilateral pons and other pain-related brain regions such as ipsilateral prefrontal cortex and contralateral subcallosal gyrus was detected during the acute spontaneous attack as compared with during headache remission(P<0.05,corrected by Monte Carlo simulation).

结果1。对照组未发现CSD;同侧TNC放电频率,CSD中>CSD后>CSD前P<0.05对侧TNC放电频率,CSD前、中、后无统计学差异(P>0.05氟桂利嗪组同侧TNC放电频率,CSD前>CSD中(P<0.05),CSD前与CSD后及CSD中与CSD后之间无统计学差异(P>0.05)。2.1关于放免测定,各组血浆CGRP、SP水平有统计学差异(P<0.05),CSD组高于对照组(P<0.05),CSD组与氟桂利嗪组、对照组与氟桂利嗪组之间均无统计学差异P>0.05各组之间同侧三叉神经节中CGRP、SP水平未见变化(P>0.05.2关于免疫组化研究,右侧三叉神经节CGRP、SP免疫阳性细胞数三组之间有统计学差异(P<0.05),多重两两比较结果CSD组大于对照组(P<0.05),CSD组与氟桂利嗪组之间、对照组与氟桂利嗪组之间无统计学差异P>0.05左侧三叉神经节CGRP、SP免疫阳性细胞数三组之间无统计学差异(P>0.05CSD组中右侧三叉神经节CGRP、SP免疫反应阳性细胞数大于左侧(P<0.05)。3.1局部一致性分析发现两组患者头痛疼痛状态较非疼痛状态脑活动发生变化的脑区有同侧脑桥以及其他疼痛相关脑区如基底节区、丘脑、扣带回、前额叶皮层等(P<0.05,蒙特卡罗模拟校正)。2功能连接分析发现疼痛状态与非疼痛状态下主要疼痛相关脑区均与同侧脑桥有功能联系P<0.05,false discovery rate,FDR校正疼痛状态与非疼痛状态比较,同侧前额叶皮层、对侧胼胝下回等疼痛相关脑区与同侧脑桥之间功能联系增强(P<0.05,蒙特卡罗模拟校正。

Results Compared with normal group and sham operated control groups, BrdU positive and Nestin positive cells were increased in the hippocampus, dentate subgranular zone and subependymal zone in operated groups after 24 hours of reperfusion of omni-cerebral ischemia.

结果 脑缺血再灌流24 h后,海马、齿状回和室管膜下区的BrdU阳性细胞和Nestin阳性细胞增多,7~10 d达到高峰,术后20 d仍有表达;在室管膜下区,BrdU阳性细胞和Nestin 阳性细胞有向皮质、海马迁移的现象。

Results: The number of BrdU-positive cells in the subventricular zone and in the subgranular zone of dentate gyrus,and the numbers of ChAT-positive cells in medial septumand diagonal band of the basal forebrain were significantly decreased in lead exposed group P

结果:铅中毒大鼠齿状回的颗粒下层、侧脑室室管膜下区的BrdU阳性细胞数、基底前脑内侧隔核和斜角带核的ChAT阳性细胞数以及大脑皮质和海马齿状回NF阳性神经元数、嗅球BrdU+NF阳性细胞数目以及学习记忆能力明显下降,GFAP阳性细胞反常升高P

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