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Result: The nm-HAP-sol present the dispersed and uniformity needle. A431cells inhibition ratio was maximizing at the 5 day in the 400mg/L nm-HAP-sol group, the 200mg/L nm-HAP-sol group and the 100mg/L nm-HAP-sol group, inhibition ratio of the 400mg/L nm-HAP-sol group, the positive control group and the 50mg/L nm-HAP-sol group was respectively (77.89±6.29)%,(77.46±8.26)% and (1.23±0.15)%. It was detected that the number of A431 cells were persistently increasing in the 50mg/L nm-HAP-sol groups while decreasing in the 400mg/L nm-HAP-sol group, the positive control group and abidingly increasing for 4 and 3 days then decreasing in the 100mg/L and 200mg/L nm-HAP-sol group by inverted microscope.

结果:超声乳化法配制的HAP纳米溶胶呈均匀分散的针状;400mg/L、200mg/L和100mg/L溶胶组对A431细胞抑制率在第5天达到各组最大值,此时400mg/L溶胶组与阳性对照组的抑制率分别为(77.89±6.29)%和(77.46±8.26)%, 50mg/L溶胶组抑制率为(1.23±0.15)%;倒置显微镜下见50mg/L溶胶组细胞数目持续增加,聚集成簇,细胞呈现多角形或梭形,胞浆透亮,而100mg/L 和200mg/L溶胶组的细胞数目分别增加至第4天和第3天开始下降,400mg/L溶胶组和阳性对照组细胞数目持续减少,细胞体积缩小,漂浮细胞增多。

Methods Monoclonal antibody against the glycophorin A was used to identify nucleated erythrocytes in the peripheral blood of pregnant women.Candidates fetal cells from 3 pregnancies at 16 to 24 weeks of gestation were isolated successfully.Sorted cells were also confirmed using Y-special probe (PY3.4) repeated sequences in maternal blood from women carrying male fetuses were detected by polymerase chain reaction.Results The PY3.4 position hybridization rates were 31.5%0 and 38% respectively in sorted GPA positive cells of 3 samples by FISHand the results of GPA+ cells of the 3 cases by PCR were positivenegative and positive respectivelywhich were manifested by the gender after aborticide.

选用红细胞特异单克隆抗体Glycophorin A结合流式细胞术成功地从3例妊娠16~24周的孕妇血中分离出NRBCs,并用Y-染色体特异重复序列DNA探针(PY3.4)荧光原位杂交及聚合酶链反应技术检测Y-染色体特异序列结果 FISH结果,3例分选出的GPA阳性细胞中PY3.4杂交阳性率分别是31.4%、0、38%;其PCR结果,分别为阳性、阴性、阳性,并在引产后得到性别证明。

Pneumonia group presented highly sensitive trend to dust acarid,and positive cases increased with the growth in age and had a positive correlativity.

哮喘组对尘螨呈高敏感趋势,而且阳性率随年龄增长而增长,呈正相关关系。

The leading allergen was dirt and next were acarid, dog hair, roach and pineapple. The total positive items was 156 and inhale allergens accounted for 62.8%. The positive rate of inhale allergens of 3~6 and 6~14 years old group was markedly higher than those of and 1~3 years old group. But to food allergens, the 3~6 and 6~14 group was markedly lower than the latter. There was 24.6%(32/130) patients founded more than 3 allergens.

阳性率最高的过敏原为屋尘,占65.5%,其次为户尘螨、狗毛屑、蟑螂和菠萝。3~6岁和6~14岁组的吸入性过敏原阳性率显著高于<1岁和1~3岁组(P.05),而食入性过敏原阳性率则显著低于后者。24.6%(32/130)患儿检出3种以上过敏原。

According the survey,the primary inhaled allergen for the asthma children is dust acarid,and the positive cases increase with the growth in age and present a positive correlativity.

本组受检哮喘儿童吸入性过敏原中,主要过敏原为尘螨,而且阳性率及过敏反应程度随年龄的增长而增长,呈正相关关系。

Results (1)The positive rates or the scorings of MMP-1,MMP-9and MVD were significantly higher in human gallbladder neoplasm than that in human chronic cholecystitis(P.01),But the positive rates of MMP-2in human gallbladder neoplasm were significantly lower than that in human chronic gallbladder cholecystitis(P.01).(2)The closely positive correlations were found among the expressive scorings ofMMP-1,MMP-9,TIMP-2,MVD and the clinicopathological type,differentiated degree,with or without metastasic tumor.

结果 (1)31例胆囊癌组织中MMP-1,MMP-9的阳性表达率及其评分和MVD计数均值(以CD34阳性计数)分别为16例(51.6%)和20例(64.5%),明显高于10例慢性胆囊炎组织1例(10.0%)和2例(20.0%),差异有非常显著性(P.01);胆囊癌组织中TIMP-2的阳性表达13例41.9%)则低于慢性胆囊炎组织9例(90.0%),差异有非常显著性(P.01)。

Results Group C had the symptoms such as reduced activity, acceded, indulge in lying and weight loss after 3 weeks of immune injection, 14 out of 16 SD rats in Group B had the same symptoms as Group C after 4~5 weeks of immune injection, the serum enzymes in model groups increased significantly compared with those of the control group, model group C was much higher than model group B; the duration shorted, amplitude decreased, multiphase wave increased in electromyogram of model groups; MRI examination revealed samples from model group B and C had one positive case each, which presented T1MI isodensity or hypodensity signal, T2MI and STIR serial hyperdensity signal, revealing muscle inflammation; all rats'skeletal muscle from model group C and 11 out of model group B had pathological changes, which exhibited striated muscle focal fiber degeneration, necrotized and inflammatory cell infiltration, interstitial vessel wall thickening, random cardiac muscle samples had 3 positive changes, which had similar changes to skeletal muscle, there was 1 positive change from lung sample.

结果:模型C组于免疫注射第3周左右开始出现活动减少,倦怠嗜卧,食欲体重下降等表现,模型B组有14只SD大鼠于免疫注射第4~5周出现上述症状,较C组为轻;模型组肌酶谱中,肌酸激酶、乳酸脱氢酶、谷草转氨酶与对照组比较明显升高,模型C组较模型B组升高更显著;模型组肌电图时限缩短,波幅降低,多相波增多;磁共振检查模型B组和C组选送标本中各有一例有阳性改变,表现为T1MI等或稍低信号,T2MI及STIR序列为高信号,提示肌肉炎症水肿改变;模型B组有11只,模型C组全部大鼠骨骼肌出现病理改变,表现为横纹肌局灶性分布的肌纤维变性炎细胞浸润,间质小血管壁增厚、扩张,随机选送的心肌标本中有3例有阳性改变,表现与骨骼肌相仿,选送的肺标本中有1例有阳性改变,表现为蛋白渗出,炎症细胞浸润和小血管改变。

Pros and cons of raising the interpolation method and is slightly different, a positive start to be positive by the interpolation of its flock, the formation of green velvet, the back and by the need to start and positive by the Cashmere and slipping under its help to by efforts and self-rod, forced to back down by the sink, forming the back of green velvet.

正反两面的插法和起绒方法稍有不同,正面的须起动正面绒经插于其下,形成绒坯,背面的须同时起动地经和正面绒经,插于其下,借助地经的努力和金属杆自重,迫使背面绒经下沉,形成背面绒坯。

But it seems there is another point which will not only watch the relationship between the hemolysis, but also RH factor is positive or negative, if it is positive there is no problem, since most citizens are positive, if negative on the trouble spots , and also looking for good this blood type in advance, for fear of massive production, this type hard to find time to find on too late.

不过好像这其中还有一点不光是看会不会溶血的关系,还要看RH因子是阳性还是阴性的,如果是阳性的就没问题,因为大多数国人都是阳性的,如果阴性就麻烦点了,还要事先找好这种血型,怕生产时大出血,这种血型很难找,到时候再找就来不及了。

The curative effect in PPD strong-positive patients was better than PPD positive patients,and the curative effect in PPD positive patients was better than PPD negative patients.

PPD试验强阳性患者的治疗疗效优于PPD试验阳性的患者,PPD阳性的患者的治疗疗效优于PPD试验阴性的患者。

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