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Objective To explore livin, MTA1 and the caspase3 protein expression, their correlation and clinical pathology in colon cancer Methods The expressions of livin, MTA1 and caspase3 protein in 88 cases of colon cancer were detected by immunohistochemistry stainingResults Livin, caspase3 protein and MTA1 positive expression rates in colon cancer tissues were more than those in tissues beside the cancer Furthermore there were obvious relevance between livin protein, MTA1 positive expression rate and degree of histodifferentiation and lymph node metabasis and between caspase3 protein positive expression rate and degree of histodifferentiation of colon cancer Caspase3 protein expression had prominent inverse correlation with the livin expressionConclusions ①Over expression of livin having inhibtion on colon cancer is one of important factors of colon carcinogenesis ②Caspase3 protein expression in colon cancer tissues is inhibited to less and has prominent inverse correlation with livin expression Accordingly, suppressing caspase3 protein activity is one of channels, by which livin promotes colon carcinogenesis ③MTA1 plays the important role in histodifferentiation degree and lymph node metastasis of colon cancer

采用免疫组织化学染色方法检测88例结肠癌组织中Livin,Caspase3 及MTA1的表达情况。结果(1)结肠癌组织中Livin的阳性表达率显著高于癌旁组织,且Livin的表达与结肠癌的组织分化程度及淋巴结转移程度显著相关。(2)结肠癌组织中Caspase3蛋白的阳性表达率显著高于癌旁组织,且Caspase3蛋白的表达与结肠癌的组织分化程度显著相关。(3)结肠癌组织中MTA1的阳性表达率显著高于癌旁组织,且MTA1的表达与结肠癌的组织分化程度及淋巴结转移程度显著相关。(4)Caspase3蛋白的表达又与Livin的表达呈显著负相关。结论(1)Livin在结肠癌组织中过表达,它可能是促进结肠癌发生的重要因素之一。(2)Caspase3蛋白在结肠癌组织中表达被抑制而降低,且与Livin的表达呈负相关。因此,抑制Caspase3蛋白的活性是Livin促进结肠癌发生的途径之一。(3)MTA1对结肠癌的组织分化及淋巴转移发挥重要作用。

Results There are 13 positive cases in 30 in situ Hybridization tissues of NPC, the p16 gene mRNA positive rate is 43.3%, and the p16 gene mRNA are positive in all of 20 cases of chronic Nasopharyngitis.

结果 30例NPC标本原位杂交阳性13例,p16 mRNA阳性表达为43.3%,3个高发家系的NPC标本无阳性表达,20例对照全部有p16 mRNA阳性表达。

By in situ hybridization, 53BP2 mRNA expression is revealed in tumor cell nests of NPC paraffin sections. The strong positive particles localize in the nuclei of tumor cells; but slight positive signals are also found in tissue cells of chronic nasopharyngitis and connective tissue, such as lymphocytes and smooth muscle cells; There are also positive signals in the nuclei of hyperplastic squamous epithelial cells. However, 53BP1 mRNA expression is not found in nuclei of tumor cells, tissues of chronic nasopharyngitis and connective tissue.

原位杂交结果显示,石蜡切片的鼻咽癌癌巢内,可见53BP2的强阳性表达信号,信号呈紫色颗粒状分布于胞核,核仁清晰可见;而在相同条件下,在慢性鼻咽炎组织和癌旁上皮的结缔组织,也可见弱表达信号,如淋巴细胞和平滑肌细胞;在增生的鳞状上皮细胞的核内,可找到强阳性信号。53BP1在鼻咽癌、慢性鼻咽炎组织和癌旁上皮的结缔组织切片上,均无表达信号。

The apparatus includes a positive contact (50) positioned so as to engage the positive terminal (42) of the battery, a negative contact (52) positioned so as to engage the negative terminal (44) of the battery, a third contact (54) positioned so as to engage the third terminal (46) and a logic circuit (72) connected to the positive, negative and third terminals.

所述装置包括:一个设置用于与电池的正端(42)连接的正触点(50);一个设置用于与电池的负端(44)连接的负触点(52);一个设置用于与电池的第三端(46)连接的第三触点(54);和一个与正触点、负触点和第三触点连接的逻辑电路(72)。

Results ①Compared to sham group, ISH and IHC of rat hypothalamic CRHshow positive cells numbers increase, positive areas enlarge and average gray scaledecreases, which reveals the expression of hypothalamic CRH neurons enhances afterPX;②The enhancement of ISH and IHC of hypothalamic CRH in PX rats aftermelatonin supplementation dose-dependently recovers, but it can't return to be normaleither in high dose group or in low dose group and either at 4th week or at 8th week;③IHC of CRH in median eminence of neurohypophysis shows positive areas enlargeand average gray scale decreases, which reveals the releasing of CRH fromhypothalamic CRH neurons increases;④ELISA of CRH in serum shows thedifferences of CRH concentrations among all groups are not significant, which indicates PX and melatonin supplementation have no significant effect on CRH levels.

结果: ①松果体摘除后大鼠下丘脑室旁核CRH 原位杂交和免疫组化的结果与假手术对照组相比均显示阳性细胞数增多、阳性面积增加、平均灰度降低,提示松果体摘除后下丘脑CRH 神经元的表达增强;②松果体摘除大鼠补充褪黑素后,下丘脑CRH 原位杂交和免疫组化染色增强呈剂量依赖性恢复,不过不论是低剂量组还是高剂量组,也不论是术后4 周还是术后8周,均不能恢复至正常;③松果体摘除后神经垂体正中隆起CRH 免疫组化结果显示阳性面积增加、平均灰度降低,提示下丘脑CRH 神经元释放CRH 增加;④ELISA 法检测血清CRH结果示各组血清CRH 浓度差异没有显著性,表明松果体摘除及补充褪黑素对血清CRH 水平没有显著影响。

Results ①Compared to sham group, ISH and IHC of rat hypothalamic CRHshow positive cells numbers increase, positive areas enlarge and average gray scaledecreases, which reveals the expression of hypothalamic CRH neurons enhances afterPX;②The enhancement of ISH and IHC of hypothalamic CRH in PX rats aftermelatonin supplementation dose-dependently recovers, but it can't return to be normaleither in high dose group or in low dose group and either at 4th week or at 8th week;③IHC of CRH in median eminence of neurohypophysis shows positive areas enlargeand average gray scale decreases, which reveals the releasing of CRH fromhypothalamic CRH neurons increases;④ELISA of CRH in serum shows thedifferences of CRH concentrations among all groups are not significant, whichindicates PX and melatonin supplementation have no significant effect on CRH levels.

结果: ①松果体摘除后大鼠下丘脑室旁核CRH 原位杂交和免疫组化的结果与假手术对照组相比均显示阳性细胞数增多、阳性面积增加、平均灰度降低,提示松果体摘除后下丘脑CRH 神经元的表达增强;②松果体摘除大鼠补充褪黑素后,下丘脑CRH 原位杂交和免疫组化染色增强呈剂量依赖性恢复,不过不论是低剂量组还是高剂量组,也不论是术后4 周还是术后8周,均不能恢复至正常;③松果体摘除后神经垂体正中隆起CRH 免疫组化结果显示阳性面积增加、平均灰度降低,提示下丘脑CRH 神经元释放CRH 增加;④ELISA 法检测血清CRH结果示各组血清CRH 浓度差异没有显著性,表明松果体摘除及补充褪黑素对血清CRH 水平没有显著影响。

With eggs and children artemia were detedcted using nested-PCR. WSSV positive products could be detected in artemia, and DNA sequence of the product accords with the designed specific amplified one according to sequence analysis. The positive rates of wild brine shrimp and filial generation were 58% and 43%, respectively. However, it seems to be no regularity between parents and filial generations positive results. It may not judge whether WSSV could vertically transmit in artemia or not.

检测结果显示:卤虫中可扩增出特异性的、与引物设计吻合的DNA片段;DNA测序分析表明,扩增片段的DNA序列与白斑综合症病毒序列一致;野生卤虫携带白斑综合症病毒的阳性率为58%,实验条件下野生卤虫的后代的阳性率为43%;同一卤虫家系中,亲代与子代携带病毒的情况不完全一致,不能确定白斑综合症病毒可否通过繁殖在卤虫世代间进行垂直传播。

Perceptively on 3d and 5d. Collagen is positive in Western blot on 9d. Aniline dyeing is positive on 12d. The control group was negative. Ad-BMP-2 transferred MSCs cultured in fibrin gel was positive in Aniline dyeing, Immunohistochemical. The therapy effect of group was better in histology, biochemistry and biomechanics in all periods than the other two groups, and was similar to the natural cartilage.

结果 ①Ad-BMP-2转基因MSC BMP-2、CollagenⅡ的PT-PCR分别在3、5d呈阳性,CollagenⅡ的Western Blot 9d为阳性,较对照组有显著差异;②转基因MSC经纤维蛋白体外三维培养后免疫组化和苯胺蓝染色呈阳性,电镜显示细胞生长良好,有基质合成;③转基因修复组各时期大体、组织形态学和组织化学成份均显著优于其它两组,12W时力学和组织学已接近正常关节软骨。

Positive Psychology has become a new force in the field of psychology. Analyzed from the angle of Positive Psychology, problems existing in the traditional mainstream psychology and why it is negative can be illustrated, which can help us to recognize, interpret Positive Psychology and make a better use of its function.

积极心理学已经成为心理学一股新的力量,从积极心理学的视野对传统主流心理学进行探讨,可以发现传统主流心理学存在的问题,理解其消极的原因,也可以帮助我们更好地认识、理解积极心理学,更好地发挥积极心理学的功能。

By the priori estimates and degree theory on the positive cone, we obtain some conditions on the existence and non-existence of positive solution, at the same time, we also discussed the properties of the positive solution.

利用先验估计和正锥上的度理论,给出了正解存在的条件,同时,也讨论正解的一些性质。

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