查询词典 polymerase

Objective To establish a method for quantitative detection of cytotoxin associated gene A positive Helicobacter pylori by competitive polymerase chain reaction.

目的 建立竞争性聚合酶链反应定量检测幽门螺杆菌细胞毒素相关蛋白基因A的方法。

Methods: A polymerase chain reaction assay with oligonucleotide primers homologous to a portion of the urease C and cytotoxin associated gene A of Hp was used to test the presense of Hp in dental plaque.

依据特异的尿素酶C 基因和 cag A基因设计引物，建立聚合酶链反应方法，检测65例慢性胃病患者不同牙齿的龈上和龈下菌斑中的Hp.6例胃Hp阳性的胃炎患者，经药物治疗后，检测口腔Hp。

The present study wastherefore designed to examine the molecular toxic effect of MC-LR on bighead carpAristichthys nobilis and zebrafish Danio rerio by using real-time polymerase chainreaction, molecular cloning and immunoblotting.

本研究以斑马鱼和鳙鱼为对象，采用实时荧光定量PCR、分子克隆和免疫印迹技术在分子水平上揭示了MC-LR对鱼类的毒理效应。

RESULTS: The polymerase chain reaction product was in concordance with target fragment. The analysis of the gene sequence proved it is the ALR gene. The overall length of the ALR gene was 378 bp, it was highly conservative in many species, for instance the Homo sapiens, the Mus musculus, the Bos Taurus and the Danio rerio.

结果：聚合酶链反应扩增结果与目的片段位置一致，测序结果表明为肝再生增强因子mRNA，基因全长378 bp，在人、牛、小鼠、斑马鱼等物种中高度保守。

The main regions (405-1 146 nt) in the glyco pro tein gene of 8202,BRV and MRV,isolated from deer,cattle and mouse in China re spectively,of rabies virus was amplified by reverse transcriptase-polymerase ch ain reaction,cloned and sequenced.

对来源于鹿、牛和鼠的狂犬病病毒野毒株82 02、BRV、MRV的G基因405～1 146位核苷酸序列进行了RT-PCR扩增、克隆和序列测定，并应用计算机软件对这3个毒株的测定序列和人源株CGX89的相应序列进行了分析比较。

In order to understand insertion/delation polymorphism of the angiotensin-converting enzyme gene in pilots,and to explore the relationship between ACE gene I/D polymorphism and the perfomance of the pilots,the polymerase chain reaction was used to determine the genotypes for an I/D polymorphism in intron 16 of the ACE gene in 118 pilots and 96 healthy subjects as controls.

为了解飞行员血管紧张素转换酶基因插入或缺失多态性情况，探讨ACE基因多态性与飞行员耐力可能的关系，用聚合酶链反应扩增技术检测118例飞行员和96例健康对照者的ACE基因I/D多态性。

The methods of DNA depuration and detection are proved to be simple,quick and accurate, which establish a good foundation for the assessment of DNA vaccine safety,and have the favourable perspective in application.

荧光定量PCR（Fluorescent Quantative Polymerase Chain Reaction，FQ-PCR技术是美国PE公司研制成功的一种高灵敏度和高精确度核酸检测方法，该法在传统PCR的基础上引入荧光标记探针，从根本上解决了PCR反应中非特异性扩增的难题[4]。

The reverse transcription polymerase chain reaction is a routine diagnostic assay for the detection of hMPV.

RT-PCR技术是hMPV主要的检测手段，hMPV感染也是哮喘急性发作和慢性阻塞性肺病急性加重的诱因。

Methods: Twelve microsatellite markers located at chromosomes 3p,9p and 14q were selected to investigate microsatellite alterations in 31 RCC specimens and their paired metastasis specimens by polymerase chain reaction-polyacrylamide gel electrophoresis-ethylene dibromide (PCR-PAGE-EB) staining and sequencing.

选取位于染色体3p、9p、14q上的共计 12个微卫星多态性标记，采用PCR-中性聚丙烯酰胺凝胶电泳-EB染色和测序的方法对31例肾细胞癌患者的原发病灶和配对的转移病灶进行MSI和LOH分析。

Methods: Twelve microsatellite markers located at chromosomes 3p, 9p and 14q were selected to investigate microsatellite alterations in 31 RCC specimens and their paired metastasis specimens by polymerase chain reaction-polyacrylamide gel electrophoresis ethylene dibromide (PCR-FAGE-EB) staining and sequencing.

选取位于染色体3p、9p、14q上的共计12个微卫星多态性标记，采用PCR-中性聚丙烯酰胺凝胶电泳－EB染色和测序的方法对31例肾细胞癌患者的原发病灶和配对的转移病灶进行MSI和LOH分析。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。