查询词典 polymerase

Objective To study the value of polymerase chain reaction in prenatal diagnosis of alpha thalassemias.

目的 研究聚合酶链反应技术在α地中海贫血产前诊断中的价值。

Objective To investigate the value of polymerase chain reaction in the diagnosis of herpes simpler keratitis.

目的 探讨PCR对HSK的诊断价值。

Methods The expression of mdr1 and MRP were measured in 55 patients with AL by reverse transcription polymerase chain reaction.

采用逆转录-聚合酶链反应的方法检测55例AL患者的mdr1及MRP的表达。

A method based on polymerase chain reaction for screening cDNA library is described.

建立了一种以PCR为基础的快速筛选cDNA文库的方法。

To study and know well the rudimentary knowledge and the basic techniques of modern Microbiology are demanded for Graduate students. The experiments including the principle and operation of phase contrast microscope and fluorescence microscope; Microphotography , the technique of photographic enlargement;Applications of polymerase chain reaction ; Construction, transformation and detection of plasmid in E.coli ; Infectivity of virus and its molecular detection ; The technique of protoplast fusion in filamentary fungi ; Induction and purification of β-galactosidase from E.coli and assay the enzyme activity; Detection of GC content and DNA-DNA hybridization in bacteria ; Production , extraction and detection of poly--hyduoxy butyrate from bacteria.

以实验教学为主，要求研究生学习和掌握一些现代微生物学研究所需的基本技术，内容包括：相差显微镜、荧光显微镜的使用和显微摄影技术，PCR技术的应用，细菌质粒的构建、转化及检测技术，病毒的侵染性及其分子检测技术，丝状真菌原生质体融合技术，大肠杆菌β-半乳糖苷酶的分离及纯化及其动力学测定技术，细菌G+C mol%的测定及细菌DNA-DNA杂交技术和聚羟基丁酸产生菌的发酵、产物提取及检测技术等。

Objective: To detect Orientia tsutsugamushi in Chigger Mites and the specimens collected from the rodents to analyse the epidemic trend of scrub typhus. To evalute the sensitivity and specificity of nested polymerase chain reaction and promote the research in molecular epidemiology of O.tsutsugamushi in the country.

摘 要 目的：检测恙螨幼虫体内及鼠类标本中恙虫病东方体携带情况，预测流行趋势，评价套式PCR反应的敏感性和特异性，以进一步开展分子流行病学研究。

Using universal primers (R16mF2/Rl and R16F2/R2) for 16S rRNA and primers (rpF1/rpR1) for ribosomal protein gene to detect phytoplasmas associated with of chinaberry tree witches'-broom from Yunnan and Guizhou Province by polymerase chain reaction.

根据植原体16S rRNA基因通用引物序列(R16mF2/R16mRl、R16F2/R16R2)及核糖体蛋白基因通用引物序列(rpF1/rpR1)，应用PCR技术对采自不同省份的苦楝丛枝病植原体进行检测，结果从两个植物上分别获得苦楝丛枝病植原体云南株系和贵州株系。

Objective To study the value of polymerase chain reaction in detection of chlamydia trachomatisfrom urogenital tract.

目的 探讨多聚酶链式反应在检测泌尿生殖道沙眼衣原体的应用价值。

The level of serum amylase, the pulmonary dry/wet weight ratio and histological changes were measured at different time points in each group. The expression of pulmonary CINC protein and mRNA were detected by immunohistochemistry and semi-quantitative reverse transcriptase polymerase chain reaction, respectively.

分别检测各组不同时间点血清淀粉酶、肺干湿重比和肺组织病理学改变，用免疫组织化学法和半定量逆转录聚合酶链反应法检测肺组织中CINC蛋白和CINC mRNA表达的变化情况。

METHODS: The acute pancreatitis model was induced by retrograde injection of 50 g/L sodium taurocholate into the bili-pancreatic duct in SD rats. Forty-eight mas-culinity SD rats were randomly divided into 2 groups: the sodium taurocholate group and the operational control group. The serum amylase level and the histol-ogical pathologic changes of pancreas were measured at different time point in each group. The expression of pancreatic CINC protein and mRNA were determined by immunohistochemistry and semi-quantitative reve-rse transcriptase polymerase chain reaction, respectively.

采用向胆胰管逆行注射50 g/L牛磺胆酸钠建立大鼠SAP模型， 48只♂SD大鼠随机分为手术对照组和SAP组，分别检测各组不同时间点血清淀粉酶和胰腺组织病理学改变，用免疫组织化学法和半定量逆转录聚合酶链反应法检测胰腺组织中CINC蛋白和CINC mRNA表达的变化情况。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。