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The chondrocytes of different generation were observed with light-microscope and transmission electron microscope for cellular growth and ultromicrostructure, with the method of MTT assay for grow curve and proliferation, with alcian blue test for GAG of ECM, with immuocytochemistry and RT-PCR (reverse transcript -polymerase chain reaction)for type Ⅱcollagen, with flow cytometry for cell life cycle ,histochemistry for S-A-β-galand so on by which to identify cataplasia and senescence of chondrocytes cultured in vitro.

对软骨细胞退变老化进行观察，采用相差显微镜观察其生长情况，透射电镜观察细胞结构，阿力新蓝染色检测胞外基质硫酸GAG含量和结构，免疫细胞化学法和RT-PCR方法检测Ⅱ型胶原鉴定软骨细胞，以MTT比色法描绘生长曲线、检测生长状态，组化法检测老化相关β-半乳糖苷酶，流式细胞仪分析细胞周期和增殖指数。3。

Polymerase chain reaction analysis of cerebrospinal fluid is the gold standard for the diagnosis of herpes simplex encephalitis.

对脑脊液的聚合酶链反应是诊断单纯疱疹性脑炎的金标准。

The gene of ESAT-6 from genome of bovine mycobacterium tuberculosis strain that from cervine tuberculosis was amplified by polymerase chain reaction and inserted into pGEM-TE vector. After sequencing, we analyzed the gene encoding ESAT-6 protein with molecular biologic software.

从患结核病鹿的肺组织中粗分离牛分支杆菌制备DNA模板，用PCR对牛分支杆菌ESAT-6基因进行了扩增，将扩增的产物连接于测序载体pGEM-TE上克隆，经测序确定无误；并使用分子生物学软件对ESAT-6基因进行了分析。

Kary Mullis of Human Heredity Department, the Cetus Company set up this technology by using Klenow DNA polymerase .

聚合酶链式反应是一种模拟DNA 聚合酶在体内的催化作用，在体外快速扩增特定DNA片段的技术，是现代分子生物学最重要的手段之一。

Under the same amplification condition of polymerase chain reaction,five different sample treatment methods for detection of HBV DNA were compared.

对多聚酶链反应扩增条件相同情况下，5种标本处理方法HBVDNA检测结果进行了比较。

Objective: To investigate the clinical value of Polymerase Chain Reaction in the diagnosis of trachoma.

目的：探讨聚合酶链反应在沙眼诊断中的临床应用价值。

Methods The genotypes of 152 patients with CI were detected by polymerase chain reaction and compared with control group.

应用聚合酶链反应技术，检测 15 2例CI患者的基因型，并与对照组比较。

In this work, we first evaluate a polymerase chain reaction based method for diagnosis of leptospirosis.

在本文中，我们首先评估了通过PCR法检测钩端螺旋体病的方法。

Objective To establish a rapid, sensitive, and specific polymerase chain reaction method for detection of Vibrio parahaemolyticus.

目的 为建立副溶血弧菌的快速、敏感、特异的PCR检测方法。

In later stages of the disease, a process called polymerase chain reaction can be used to confirm diagnosis.

在稍后阶段的疾病，这个过程称为聚合酶链式反应可以用来确定诊断。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。