查询词典 polymerase

Methods:180 HCV patients and 40 healthy controls were selected for the research, and their serum BAFF levels were detected by ELISA. The level of serum HCV-RNA in HCV patients was detected through fluorescent quantitative polymerase chain reaction and analyzed along with the clinical data of HCV patients.

选取180名HCV感染者及40名健康对照，应用酶联免疫吸附法测定血清中BAFF含量，荧光定量聚合酶链反应检测HCV感染者血清中HCV-RNA的水平，并结合HCV感染者临床情况分析。

Objective To improve the efficiency of long distance polymerase chain reaction by using betaine.

目的：利用甜菜碱优化长距离PCR，提高血友病A基因倒位检测效率。

F508 mutation detection on the single blastomere was performed with nested polymerase chain reaction.

同时利用套式聚合酶链反应技术，对活检所得的单个卵裂球分别进行ΔF508 突变基因检测。

BOULE transcript levels in the testes of 41 azoospermic patients were examined by quantitative competitive-reverse transcription-polymerase chain reaction.

我们以定量竞争性反转录聚合连锁反应的方法定量 41 位无精虫症患者睪丸内 BOULE 的转录量。

Characterization of complementary DNAs derived from braconid wasp ovaries identified genes encoding subunits of a viral RNA polymerase and structural components of polydnavirus particles related most closely to those of nudiviruses—a sister group of baculoviruses.

基因编码的RNA聚合酶亚组和多去氧核糖核酸病毒粒子的结构组件，与nudiviruses有着密切的联系。而通过来自小茧蜂卵巢互补DNAs的表征可以将两者识别出来。

Besides its simpliness and quickness, the method also has characters of specificity and sensitivity. It has a great importance to detect and diagnose Brucellosis by Polymerase Chain Reaction.

该奶牛布鲁氏菌病病原PCR快速检测方法的建立，对奶牛布鲁氏菌病的检疫及快速诊断具有十分重要的意义。

Methods The experimental TBI model was established by bumpiness of free falling body according to Feeney′s.The rats′cerebral edema were imaged with magnetic resonance image,The changes of brain water content and permeability of blood-brain barrier were measured by the methods of wet and dry weight and Evans blue fluorometry.The expression of AQP-4 mRNA was examined by reverse transcriptase polymerase chain reaction.

按照改进的Feeney自由落体撞击法建立大鼠创伤性脑损伤模型，用磁共振成像对大鼠脑水肿进行检测，干/湿比重法和伊文思蓝测定法观察大鼠TBI后不同时相脑组织含水量和BBB通透性的变化，并采用逆转录聚合酶链反应法检测AQP-4 mRNA的表达，蛋白质免疫印迹法检测AQP-4蛋白的表达。

After synthesis of the DNA polymerase and replication of DNA,the viral capsid and glycoproteins are synthesized.

在DNA多聚酶合成DNA复制后，病毒衣壳和糖蛋白合成。

The expressions of HO-1 and HO-2 mRNA were detected using reverse transcription polymerase chain and the relative amount of CO released into the media was quantitated as carboxyhemoglobin by enzyme linked immunosorbent assay.

通过逆转录聚合酶链式反应和免疫细胞化学法检测HO-1、HO-2 mRNA和蛋白的表达，酶联免疫法检测培养上清液中碳氧血红蛋白含量，四唑氮比色法检测细胞增殖。

DNA were abstracted by means of phenol chloroform, amplification and cataphoresis test of polymerase chain reaction of target DNA,primer sequence of gene segment amplification of 5-hydroxytryptamine (5-HT) 2A receptor gene T102C: 5'-TCT GCT ACA AGT TCT GGC TT-3', 5 '-CTG CAG CTT TTT CTC TAG GG-3'; Reaction system of 50 μL, DNA 0.5 μg,Primer 50 pmol,TagDNA enzyme 2U were added with dNTP to the final concentration of 200 μmol/L.

应用常规苯酚氯仿法抽提DNA，目的DNA的聚合酶链反应扩增及电泳检测，5-羟色胺2A受体基因T102C基因片断扩增的引物序列：5'-TCT GCT ACA AGT TCT GGC TT-3'。5'-CTG CAG CTY TTT CTC TAGGG-3'；采用50 μL反应体系，DNA 0.5 μg，引物50 pmol，TagDNA酶2U加dNTP至终浓度200μmol/L。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。