查询词典 polymerase

Polymerase chain reaction ; agglutinin ; pneumonia ; mycoplasma

聚合酶链反应；凝集素；肺炎；支原体属

Glossy ganoderma decoction may protect the liver from Amanita mushroom poisoning. Its mechanism may be related to the increase of the activities of hepatocyte RNA polymerase.

灵芝煎剂对鹅膏毒蕈中毒肝有明显保护作用，其机制可能与提高肝细胞RNA多聚酶活性有关。

The Culture of Green beaus in extract solution of seven poisonous mushrooms resulted in different effects. There are obvious difference among the content of the proteins in Green beaus which were cultured in extract solution of seven poisonous mushrooms, and it is thought that α-amanitin in extract solution of these mushrooms can inhibit the function of RNA polymerase Ⅱ in eukaryoctic cells and further inhibit the synthsis of protein.

用鹅膏菌属含α-毒伞肽的毒素粗提液培养新鲜绿豆，结果在36小时后，各种不同毒苗的毒素粗提液培养的绿豆生长情况有明显不同，用紫外吸收法测定蛋白质含量，发现毒素粗提液培养的绿豆细胞中蛋白质含量比蒸馏水培养的有明显下降，这表明α-毒伞肽的作用机理的确是通过抑制RNA聚合酶Ⅱ而寻致蛋白质合成减少。

Amato and Russello applied a straighiforward polymerase chain reaction test to determine the sex of all the captive St.

阿马托和罗素采用一种直接的聚合酶链锁反应试验来确定所有从圣文森特小岛和巴巴多斯捕获的79只圣文森特亚马逊鹦鹉的雌雄。

AIM: To explore the role of poly-polymerase in the cultured endothelial cell apoptosis induced by angiotensin Ⅱ.

目的： 探讨体外AngⅡ在引起血管内皮细胞凋亡过程中细胞内多聚聚合酶的活性变化情况。

A pair of primers was designed according to the coat protein gene of Arabis mosaic viru and used to amplify a 990 bp partial gene of the cp by reverse transcription-polymerase chain reaction, the 990 bp partial gene was cloned into the pEGX-9p-1, a prokaryotic expression vector fused with GST. The recombinant vector was transformed into E. coli BL21. The fusion protein was successfully expressed in E. coli BL21 induced with the IPTG at 37℃.

依据报道的ArMV外壳蛋白基因(coat Protein， Cp)序列设计引物，通过RT-PCR 扩增得到长约990bp的ArMV cp基因亲水基团部分片段，将目的片断克隆到原核表达载体pEGX-6p-1中，构建ArMV cp基因与GST蛋白融合表达载体pEGX-cp，重组载体化大肠杆菌BL21，经IPITG诱导后，融合蛋白GST-cp得到了特异表达。

Endometrial carcinoma ; human expression array ; gene expression ; polymerase chain reaction

子宫内膜肿瘤；人表达阵列；基因表达；聚合酶链反应

Methods Sequence specific primers-polymerase chain reaction was used for the detection of genotypes and alleles of rs3850641 A/G loci in OX40L gene in 187 PACS cases (≤55 years) and 138 controls. Diseased vessel numbers and stenostic severity integral by coronary arteriogram and serum levels of sOX40L, C-reactive protein and vascular cell adhesion molecule-1 (VCAM-1) by enzyme-linked immunosorbent assay and nephelometry were measured. The relationship between OX4OL gene variants and PACS risk, SSI as well as plasma levels of three cytokines were analysed.

采用聚合酶链反应－序列特异性扩增技术检测187例年龄≤55岁、早发急性冠状动脉综合征患者和138例冠状动脉造影正常者rs3850641位点A/G变异的基因型、等位基因及其分布频率；采用冠状动脉造影测量病变血管支数和狭窄程度积分；应用ELISA法测试血浆sOX40L、血管细胞黏附分子(VCAM-1)；应用散射比浊法测试血浆C反应蛋白水平；分析OX40L基因变异与ACS风险、冠状动脉病变程度和血浆3个细胞因子之间的关系。

Methods\ 62 cases of astrocytoma and 10 cases of the meningoma were detected by differential polymerase chain reaction.

肿瘤的发生和发展是由多种癌基因和抑癌基因改变的积累造成。

The genome DNA was extracted from the red blood cells or blood of the infected sheep and goats which include as isolates of ovine Babesia from these different geographic areas. The gene specific primers were devised according to the pubulished 18srRNA sequence at GenBank. The target DNA segment was amplified by polymerase chain reaction, the PCR product was ligated into PGEM-Teasy vector.

用分离于四个地区的羊的巴贝斯虫感染实验动物，自感染羊的红细胞或全血中提取基因组DNA，根据GenBank中已发表的巴贝斯虫18S rRNA序列设计两对羊巴贝斯虫特异性引物，通过PCR扩增目的基因片段，将扩增产物连接到PGEM-Teasy载体中，经酶切鉴定，PCR分析，进行序列测定，结果显示羊巴贝斯虫四个分离株的18S rRNA基因大小为1800bp左右。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。