查询词典 polymerase

Objective To explore effects of mutations in the tyrosine methionine aspartic acid aspartic acid motif of hepatitis B virus polymerase gene on lamivudine therapy.

目的 探讨乙型肝炎病毒多聚酶酪氨酸蛋氨酸天冬氨酸天冬氨酸基序变异对拉米夫定抗病毒疗效的影响。

The RNA polymerase binding site is adjacent to OR2. This explains how repressor autogenously regulates its own synthesis.

它们排在抑制子的一个位点上，该位点与DNA 磷酸基因邻近，而这个磷酸基因也与RNA聚合酶邻近。

Prawn white spot bacilliform virus ; polymerase chain reaction; molecular beacon probe

对虾白斑杆状病毒； PCR ；分子信标探针

After treatment of benzamide which is an inhibitor of poly polymerase,β-galactosidase activity assays and PCR analysis were performed to test the deletion of LacZ gene and c-Ha-T24ras gene. The results showed that:(1)LacZ gene was deleted from both A13.4-NIH3T3 cells and B12.7-NIH3T3 cells, but neigher from A13.4-HeLa cells nor B12.7-HeLa cells. So it is possible that there is cell line specificity in gene deletion induced by BA.(2) In A13.4-NIH3T3 cells, LacZ gene and c-Ha-T24ras gene were completely deleted at the same time.(3) The transcription activity of exgenous DNA fragment may have same effect on its sensitivity to the deletion induced by BA.

用聚ADP核糖聚合酶的NAD位点抑制剂苯甲酰胺分别处理四种转化细胞后，检测细胞中整合的外源LacZ基因与c-Ha-T24ras基因的删除情况，结果如下：BA诱导的基因删除可发生于NIH3T3转化细胞系，但不能发生于HeLa转化细胞系；位于同一外源表达载体上的LacZ基因与c-Ha-T24ras基因的删除过程是同步的；外源整合DNA片段的转录强度可能直接影响其被BA诱导删除的敏感性。

A malignant transformant line 3T3 (T24) was obtained by transfection of mouse fibroblast NIH3T3 cells with pMAMneo-T24-ras plasmid. 3T3 (T24) cells were cultured continuously in the presence of benzamide , an NAD site inhibitor of poly polymerase .

将12位密码子突变的活化ras癌基因全cDNA序列克隆到真核表达载体pSV2neo和pMAMneo上，获得了相应的重组质粒pSV2neo-T24-ras和pMAMneo-T24-ras，将pMAMneo-T24-ras质粒利用基因转移方法导入小鼠成纤维细胞NIH3T3中，活化的ras癌基因可使受体细胞发生恶性转化。

Study on glutenin subunits,GMP content and their relationships with(T.aestivum L.)With the analysis of high and low glutenin subunits of common wheat(Triticum aestivum L.)in China,the correlations between glutenin compositions,glutenin macropolymercontentand breadmaking quality were studied.Also a rapid and efficient detection method of geneticpolymorphism at Glu-D1y loci in wheat was established using polymerase chain reaction.

分析了100份小麦农家品种和241份育成品种的高、低分子量谷蛋白亚基的组成以及有关的品质性状，研究了高分子量谷蛋白亚基频率的变化，用单向一步SDS-PAGE的方法分离LMW-GS，并对我国小麦品种进行初步分类；在亚基水平上研究了不同的亚基或亚基组合对品质性状的影响；在聚合体水平上研究了谷蛋白大聚合体的含量与其他烘烤品质性状的相关性及其在面包体积预测中所起的作用；在基因水平上利用PCR技术研究了1D染色体上控制y-型谷蛋白亚基的Glu-Dly位点基因的多态性。

Methods : We subjected C57BL/6J mice to CS (whole body exposure, 90 min/day) for various periods, and used laser capture microdissection to isolate bronchiolar epithelial cells for analysis of mRNA by quantitative reverse transcription-polymerase chain reaction.

研究方法：研究者将 C57BL/6J 小鼠分别在不同时间周期暴露于CS（全身暴露，90分钟/天），用激光捕获显微切割技术分离细支气管上皮细胞，然后用定量逆转录多聚酶链反应分析上皮细胞的mRNA。

In the RAPD system of Bryaceae the optimal concentration of template DNA was 2ng/μl, primer 0.4μmol/L, dNTP 0.2mmol/L, Mg2+ 2mmol/L, Taq DNA polymerase 1.25U, total volum 25μl. Several DNA samples were selected to choose the compatible primers.

通过单因素和正交试验对影响RAPD的因素进行考查，建立了优化的RAPD反应体系：dNTP为0.2mmol/L，随机引物0.4μmol/L，Taq DNA聚合酶1.25U，Mg2+为2mmol/L，模板DNA为50ng，扩增体系为25μl。

Methods The primer design is referring to thearticleof Bulter. Over 120 samples provided by the laboratoryare detected to verify the concordant results between miniSTR andcommon STR. We construct the miniSTR multiplex system usingdomestic Taq DNA polymerase, and detect the results by ABI—310Genetic Analyzer. We have alsostudied the sensitivity, accuracy, species-specificity and different PCR outcome under each annealtemperature.

方法参照Bulter等对THO1、TPOX、CSF1PO三个基因座miniSTR的引物设计，选用本教研室提供的123例无血缘关系个体的血样，进行THO1、TPOX、CSF1PO三个基因座PCR-miniSTR银染检测，检测结果与商品化试剂盒的检测结果进行一致性检验；采用国产DNA聚合酶构建miniSTR荧光标记复合扩增体系，用美国ABI-310基因分析仪进行检测；对该荧光标记复合扩增体系的灵敏度、准确性、种属特异性、不同退火温度下复合扩增的效果、陈旧血痕DNA的检测等进行研究。

The result showed that we have established a ITS-PCR system most suitable for Buxus sinica var. parvifolia, which came out total volume 50μL reaction system, was as follows: 2.0mmol/L Mg(superscript 2+), 0.3μmol/L primer, 0.3mmol/L dNTP, 240 ng/50μL DNA template, 1.75 U/50μL TaqDNA polymerase and the anneal temperature with 56℃.This optimized ITS-PCR system might ensure the purity and quality of ITS-PCR production. In this study, the length of the ITS fragment of Buxus sinica var.

实验结果表明，在总体积50μL的反应体系中，建立了最佳ITS-PCR扩增条件：Mg(上标 2+)浓度2.0mmol/L、引物浓度0.3μmol/L、dNTP浓度0.3mmol/L、DNA模板浓度240ng/50μL、TaqDNA聚合酶的用量1.75U/50μL和退火温度56℃，该优化体系保证了珍珠黄杨ITS-PCR产物的纯度和质量要求。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐