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Anaphylactoid purpura ; HLA ; polymerase chain reaction

过敏性紫癜；白细胞抗原；聚合酶链反应

Anaphylactoid purpura ; children ; Hans ; HLA-DQA1 gene ; polymerase chain reaction-sequence specific primer

过敏性紫癜；儿童；汉族；人类白细胞抗原DQA1基因；聚合酶链反应-序列特异性引物

Design, Setting, and Patients After exposure to an index patient whose fatal illness was characterized by fever and hemorrhage at a primary care hospital and regional tertiary care hospital's isolation ward, secondary cases with febrile illness who were suspected of being exposed were tested for antibodies against Anaplasma phagocytophilum and by polymerase chain reaction and DNA sequencing for A phagocytophilum DNA.

HGA1990年在美国被创造，在欧洲被创造的时间则是在1997年。据报道，每年该疾病的感染人数在持续增加。有关数据显示，该种疾病在风行地区的感染率高达15%至36%，这意味着对该种疾病的诊断由于感染程度稍微或者是患者没有症状经常被遗漏。

Design, Setting, and Patients After exposure to an index patient whose fatal illness was characterized by fever and hemorrhage at a primary care hospital and regional tertiary care hospital's isolation ward, secondary cases with febrile illness who were suspected of being exposed were tested for antibodies against Anaplasma phagocytophilum and by polymerase chain reaction and DNA sequencing for A phagocytophilum DNA.

HGA1990 年在美国被发现，在欧洲被发现的时间则是在1997年。据报道，每年该疾病的感染人数在持续增加。有关数据显示，该种疾病在流行地区的感染率高达15%至 36%，这意味着对该种疾病的诊断因为感染程度轻微或者是患者没有症状常常被遗漏。

Design, Setting, and Patients After exposure to an index patient whose fatal illness was characterized by fever and hemorrhage at a primary care hospital and regional tertiary care hospital's isolation ward, secondary cases with febrile illness who were suspected of being exposed were tested for antibodies against Anaplasma phagocytophilum and by polymerase chain reaction and DNA sequencing for A phagocytophilum DNA.

2006年11月9日与17日之间在中国安徽省的一家地区医院中，为数9人的具有发烧和其它症状的病人被诊断患有HGA。这些病人都没有被蜱叮咬过。但所有这9位病人都在指标病例死亡前12小时内与其有过接触，该指标病例被怀疑患有致命性HGA，她出现了大出血并接受了气管内插管（即将一根柔韧的塑胶管子置放在气管中以帮助肺部的通气。）。

80 Individuals of ATA-A F2 plants were used for RAPD molecular mapping of the monoecious gene by bulk segregant analysis. 840 random primers were chosen for polymerase chain reaction amplification between monoecious pool and andromonoecious pool, the results showed that: primer S128 was tightly linked to the monoecious character, and primer S493 was tightly linked to the andromonoecious character.

以甜瓜单性花系ATA-A系与两性花品种YJX-1的杂交F2代的80个单株为材料，利用群分法进行单性花基因有关RAPD分子标记研究，筛选分析了840个10bp随机引物的PCR扩增谱带，结果表明，随机引物S128在1180bp，S493在930bp处产生与性别相关的特异性条带，S128的特异条带与单性花性状相关，S493的特异条带与两性花性状相关。

A personal interview was done and material for anogenital HPV DNA detection was obtained with swabs. HPV DNA was detected by polymerase chain reaction.

结果：600例研究对象的HPV DNA总阳性率为14.50%（87/600），其中性病患者HPV阳性率［23.90%（38／159）］显著高于非STD就诊者［11.11%（49／441）］（P0.05）。

In the present study, differential display polymerase chain reaction was used to isolate three antennal -specific mRNA from the moth of cotton bollworm. Sequence analysis indicated that A4-1 clone was homologous to the glutathione S-transferase family of proteins.

利用差异显示PCR的方法分离得到了3条棉铃虫触角专一性的mRNA片段，在GenBank中进行同源搜索表明，克隆A4-1与昆虫谷胱甘肽-S-转移酶基因家族具有很高的同源性。

Methods With polymerase chain reaction-single-strand conformation polymorphism combining argentation and glue retrieval, DNA sequencing, and restriction fragment length polymorphism, the SNP of the 50 exons in all the coding regions of ABCA1 gene was detected in 112 patients with established diagnosis of coronary heart disease.

通过聚合酶链反应－单链构象多态性分析结合银染后胶回收、DNA测序和限制性内切酶酶切分析方法检测112例明确冠心病诊断患者的ABCA1基因全部编码区50个外显子的SNP。

An optimized PCR reaction system for ISSR analysis was established:PCR was performed in a 20μl reaction mixture with 5μmol/L DNA, 2.5mmol/L Mg~(2+), 0.3mmol/Lof each dNTP, 300nmol/L primer, 1U Taq polymerase and 1×Buffer. Using 9 ISSRprimers,the genetic diversity among 23 Arrhenatherum elatius L.

3遗传多样性研究结果表明：通过模板DNA、引物、Mg~+、dNTPs、Taq酶五种ISSR反应体系的影响因素进行L_(16)(4~5)正交试验，建立了高燕麦草ISSR反应体系：20μl的反应体积中含有模板DNA 5μmol／L，引物300nmol／L，Mg~+2.5mmol／L，dNTP 0.3mmol／L，Taq酶1U及1×Buffer。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐