查询词典 polymerase

In this study, the cDNA fragments of G-L intergenic region and polymerase activity module in L gene of two street rabies virus were cloned and sequenced. The nucleotides sequence of these cDNA fragments mentioned above and their deduced amino acid sequence were compared with that of rabies virus strains, rabies-related virus strains and other Rliabdoviridae published previously. The result show that the homology of nucleotide sequence of G-L IGR among MRV, DRV is 83.3%, compared with other rabies-related virus strains, the homology of G-L IGR is between 58.3%-91.7%. the length of G-L IGR of rabies virus is different in Rhabdoviridae.

本实验对狂犬病病毒野毒株MRV、DRV的G-L间隔区以及L基因聚合酶活性部位进行了克隆和测序，将所测定的结果和推定的氨基酸序列与国内外公开发表的狂犬病病毒其它固定毒株、野毒株和同科不同属的病毒的相应部分进行了分析比较，结果表明MRV、DRV G-L IGR核苷酸序列的同源性为83.3％，与其它毒株的同源性在58.3％～91.7％之间；狂犬病毒G-L间隔区序列的长度在弹状病毒科不同病毒属之间有所不同。

Methods Blood cells of total of 77 cases with senile cataract and 76 controls were detected for GSTM1 gene, and the subcapsular epithelial cells of 22 cataract lenses were also detected for GSTM1 gene.

应用多聚酶链式反应（polymerase chain reaction， PCR）方法对77例老年性白内障患者及76例健康对照者外周血细胞进行GSTM1基因检测，并对其中的22例老年性白内障晶体上皮细胞进行GSTM1基因检测。

Mitochondrial coxI,atp6 and orf25 DNA fragments for 12 species, which belong to two subgenera Pinus and Strobus of the genus Pinus, were amplified by polymerase chain reaction using gene specific primers. Their partial sequences were obtained by directly sequencing the PCR products with the same sets of primers.

本研究利用PCR和PCR产物直接序列分析技术，获得了分属于松属两个亚属Pinus和Strobus12个松树种coxI，atp6和orf25三个线粒体基因DNA片段的序列。

Polymerase chain reaction amplification of a portion of a gene encoding a surface protein of Wolbachia was used to detect the Wolbachia infection of the population of Pteromalus puparum and Nasonia vitripennis by universal primers of wsp gene and the specific primers for wsp genes of A and B Wolbachia supergroup.

采用Wolbachia 的通用引物及A和B大组特异性引物对蝶蛹金小蜂Pteromalus puparum和丽蝇蛹集金小蜂Nasonia vitripennis体内Wolbachia的wsp基因进行PCR扩增及序列测定，并对测定的序列进行了同源性比较和基因特征分析。

Abstract］Objective: To distinguish Taenia asiatica from Taenia saginata by cox1 gene fragment sequence amplified with conventional polymerase chain reaction method.

目的：用cox1基因片段的PCR技术对亚洲带绦虫和牛带绦虫的快速鉴别。

DNA fingerprinting for Taraxacum mongolicum and its adulterants of six species of Compositae was demonstrated with random primed polymerase chain reaction including arbitrarily primed PCR and random amplified polymorphic DNA.

采用分子生物技术包括任意引物聚合酶链式反应和随意引物扩增的多态性DNA方法扩增蒲公英及其6种土公英混淆品的基因组DNA，获得清晰可靠的DNA指纹图谱。

Chinese drug ; identification ; Taraxacum mongolicum ; polymerase chain reaction ; DNA fingerprinting

药材鉴定；蒲公英；聚合酶链式反应； DNA指纹图谱

For most viruses,there is aneed for antimicrobials that target unique viral molecular properties.Acycloviris one such drug.It is activated into ahuman herpesvirusDNA polymerase inhibitor exclusively by HHV kinases and,thus,does not suppress other viruses.Here,we show that ACV suppresses HIV-1in HHV-coinfected human tissues,but not in HHV-free tissue or cell cultures.However,addition of HHV-6-infected cells renders these cultures sensitive to anti-HIV ACV activity.We hypothesized that such HIV suppression requires ACV phosphorylation by HHV kinases.Indeed,an ACV monophosphorylated prodrug bypasses the HHV requirement for HIV suppression.Furthermore,phosphorylated ACV directly inhibits HIV-1reverse transcriptase,terminating DNA chain elongation,and can trap RT at the termination site.These data suggest that ACV anti-HIV-1activity may contribute to the response of HIV/HHV-coinfected patients to ACV treatment and could guide strategies for the development of new HIV-1RT inhibitors.

对大多数病毒而言，都需要有针对其分子特性的靶向杀毒剂阿昔洛韦就是这样一种靶向药物在人疱疹病毒酶的特定作用下，阿昔洛韦被激活成为人疱疹病毒DNA聚合酶抑制剂，因此不能再抑制其它的病毒我们的研究发现阿昔洛韦在共感染人疱疹病毒的组织中可以抑制HIV-1，但在无人疱疹病毒感染的组织或细胞中无此作用然而，加入人疱疹病毒-6感染的细胞却使得其对抗HIV的阿昔洛韦变得敏感我们推测这种抑制作用依赖于人疱疹病毒酶导致的阿昔洛韦磷酸化实际上，单磷酸化的阿昔洛韦前体药物无需人疱疹病毒的参与即可抑制HIV此外，磷酸化的阿昔洛韦能直接抑制HIV-1逆转录酶，将其阻止在终止位点，从而终止DNA链的延长这些结果提示阿昔洛韦的抗HIV-1活性决定了艾滋病病毒/人疱疹病毒共感染的患者对阿昔洛韦的治疗反应，也有助于开发新的HIV-1逆转录酶抑制剂

Objective To evaluate the therapeutic effect of azithromycin on infant toxoplasmosis patients by using fluorescence quantitative polymerase chain reaction.

目的探讨荧光定量聚合酶链反应用于评价阿奇霉素治疗儿童弓形虫病的效果。

Methods The expression plasmid pSVvWF containing full-length cDNA of vWF was used to site direct mutagenesis by polymerase chain reaction and transitorily expressed in the COS-7 cells, vWF:Ag in the supernatant and the cell lysate between wild type and pSVAla737Glu vWF were measured.

用聚合酶链反应的方法对野生型的vWF全长表达质粒进行体外定点诱变，在COS-7细胞中进行表达。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐