查询词典 polymerase

To date, three kinds of reverse genetics systems, in vitro transcription system, system induced by endogeny T7 RNA polymerase and system induced by RNA polymeraseⅡ, were used to research IBDV. But these systems have some shortcomings in the efficiency and convenience of virus rescue.

目前用于IBDV研究的体外转录、基于T7RNA聚合酶的体内转录、RNA聚合酶Ⅱ体内转录等反向遗传操作系统在病毒拯救效率和操作简便性等方面仍然存在着一些问题。

Based on the fact that the dye probe can intercalate DNA duplex to emit fluorescence, a novel method for detection of DNA polymerase has been developed. The polymerization process has been indicated by fluorescent signal in real-time. Based on this assay, the influence of anti-tumour drugs on the activity of polymerase has been investigated.

利用染料探针技术，建立了一种简便快捷地实时监测聚合酶活性的方法，探讨了三种抗肿瘤药物对聚合酶活性的影响，为以聚合酶为作用靶标的抗肿瘤新药的开发及筛选提供了一种新的思路。

The results of these early research work showed that RNA polymerase Ⅰ transcription was localized in the nucleoli and RNA polymerase Ⅱ and Ⅲ in the nucleoplasm.

当时的研究结果显示：RNA聚合酶Ⅰ的转录发生在核仁内，RNA聚合酶Ⅱ和RNA聚合酶Ⅲ的位于核质中。

The results of these early research works showed that the localization of RNA polymerase I transcription was in the nucleoli and that of RNA polymerase II and Ⅲ in the nucleoplasm.

研究结果显示：RNA聚合酶Ⅰ的转录发生在核仁内，RNA聚合酶Ⅱ和RNA聚合酶Ⅲ的转录发生在核质中。

The transcription localizations of these polymerases have been studied since early 1960s. The results of these early research works showed that the transcription of RNA polymerase I was localized in the nucleoli while that of RNA polymerase Ⅲ in the nucleoplasm.

自上个世纪六十年代初期，人们相继运用细胞化学染色、电镜放射自显影等进行研究的结果表明：RNA聚合酶Ⅰ的转录发生在核仁中，RNA聚合酶Ⅲ的转录发生在核质中。

To compensate the descent of pH for the heat-up, exalting the Tris-HCl buffer's pH can avoid the abscission of the purine and make the long sequence PCR go on smoothly. The GC-rich template's secondary structure is complex and stable. The organic reagent methylamine contributes to uncoil the secondary structure.The pfu DNA polymerase has more fidelity and thermostability than Taq DNA polymerase.

高GC含量使模板二级结构复杂而稳定，一般热启动难于解开，而变性温度过高、时间过长又不利于DNA聚合酶的活性作用发挥，于是借助能缓释模板二级结构的有机试剂甲胺扩增高GC含量模板。pfu DNA聚合酶比Taq酶有更高的保真复制性能和热稳定性，更有利于高GC含量模板的扩增。

A pair of primers were designed based on M1 gene sequence of known H5N1 Avian influenza virus.M1 gene was cloned from total RNA,extracted from tissue of H5N1 subtype virus inoculated embryo by reverse transcriptase-polymerase chain reaction using high proofreading polymerase(Pyobest~TM DNA Polymerase),and expressed using Invitrogen champion~TM pET directional TOPO expression system.Recombinant protein containing polyhistidine(6xHis) tag in N-terminal about 29.8kDa in size,wac obtained and purified.

根据已发表的禽流感病毒M1基因序列设计合成PCR克隆引物，自接种H5N1亚型病毒的鸡胚组织中提取RNA，反转录后采用高可信度DNA聚合酶经PCR扩增M1基因，采用Invitrogen定向表达系统(ChampionTMpET directional TOPO expression system)进行克隆表达，纯化获得N末端携带多聚组氨酸标签的重组蛋白，分子量约29.8 kDa。

All index proved that the P5776 cDNA library constructed in this research had high quanlity and could be used for further ESTs sequencing and analyzing. The amplified library of P5776 was screened on white-blue plates, and more than 5,000 white-clones were selected to culture in 96-well plates, then the high quanlity plasmids of white-clones were extracted by traditioal alkaline-lysis method using the Vitagene?96-easy plasmid extract kit. Through PCR primered by T3( the vector has T3 RNA polymerase promoter ), the target fragments of inserts were get. Then the inserts were taged by four different fluorescence-dye, and fractionated by capillary in ABI Prism? sequencer. More than 5,000 5′-ESTs had been sequenced and through rough selecting, 4,747 ESTs were remained for further research. Among the remained ESTs, more than 90% have longer lenth than 500bp, and there are less than 5% unable-read bases in every sequence.

将扩增好的P5776 cDNA文库铺布蓝白斑筛选平板，挑取其中的白斑经活化培养后，运用Vitagene 96-easy质粒DNA制备试剂盒以碱裂解法共提取出58 板(96孔板)，即5，000 余个重组子克隆的高质量质粒DNA；提取好的质粒以T3(连接载体上具有该引物RNA聚合酶启动子)为引物经PCR(Polymerase Chain Reaction，聚合酶链式反应)扩增出重组子插入片段，标记上荧光染料后在ABI Prism 3100 测序仪上经毛细管电泳完成了5，000 余条5′端EST 序列的测定工作，初步筛选出4，747 条质量较好的EST序列，经筛选得到的EST中90%以上具有大于500bp 的可读序列，每一EST序列中不可读碱基数小于5%。

Furthermore, by using two-colored probes to study the relationships between RNA polymerase Ⅲ and RNA polymerase I, we observed that the signals of the two RNA polymerase were very close.

同时，应用这一研究方法对RNA聚合酶Ⅰ的转录发生在核仁中的结论进行了确证，并进而应用双探针标记荧光原位杂交的方法对RNA聚合酶Ⅲ和RNA聚合酶Ⅰ转录位点的关系进行了初步探讨，结果显示两者的转录位点之间非常接近，并且有伴随发生的趋势。

In this study, multiple primer polymerase chain reaction , consensus primer PCR and in situ hybridisation with biotin-labelled DNA probe (using domestically-produced agents) were applied and the sensitivity, specificity as well as practical value of each method were compared.

本实验采用了国内外领先的多重聚合酶链反应（polymerase chain reaction，PCR）和共用引物PCR法及国产生物素标记探针原位核酸杂交法，比较和估价了三种方法的灵敏度，特异性和在实际工作中的应用价值。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。