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Pocine interferon α mature protein genes were amplified by reverse transcription polymerase chain reaction, and the recombinant replication-defective human adenovius serotype 5 plasmid pAd-poIFN-α was constructed. When the recombinant plasmid pAd-poIFN-α was linearized with PacI, and then transferred into HEK-293A cells, the virus plaque was isolated and purified in HEK-293A cells by three of plaque purification passages.

本研究利用RT-PCR方法扩增猪α干扰素成熟蛋白基因后构建了重组腺病毒质粒pAd-poIFN-α，经PacI酶切后转染HEK-293A细胞，3次噬斑纯化后获得了重组腺病毒rAd-poIFN-α。

Among the products of these genes are enzymes that interfere with host gene expression and a phage RNA polymerase.

这类基因的表达的产物中，有干扰宿主基因表达的酶和一个噬菌体的RNA聚合酶。

Urogenital swabs were inoculated on the conventional solid media, new solid media and urea liquid media as such.⑤Synovial fluid, synovial-membrane biopsy samples, blood, urogenital swabs and pharyngal swabs from RA were examed the presence of mycoplasmas using culture and Polymerase Chain Reaction assay.

46例泌尿生殖道标本直接划在固体培养基表面后洗脱在Uu液体培养基和Mh液体培养基中；另20例标本直接划在无青霉素的固体培养基后洗脱在Uu液体培养基中。

We performed reverse transcriptase polymerase chain reaction in the 10th generation of treated cells to examine the expression of BDV phosphoprotein.

转染细胞经培养10代后仍然表达目的蛋白，成功建立稳定表达体系。

The test was divided into three groups randomly as yh-16 - experiment group, pbs- blank control group and rpmi-1640 medium - positive group.③in the following 24h、48h and 72h interventions, the morphous of ec was observed by photology inverted microscope, the cytoactive was detected by method of mtt and the cell cycle was investigated by flow cytometry.the expression of vegf was dectected by real-time fluorescent quantitate polymerase chain reaction(real-time pcr).

以有效浓度的yh-16作用于ec，在不同时相点光镜下观察ec形态，mtt检测细胞活性，流式细胞术检测细胞周期，实时荧光定量pcr检测vegf的表达量。

The major gene of CTXΦ receptor Tcp (toxin-coregulated pilus), tcpA, was detected by polymerase chain reaction analysis.

然而在本实验室研究中，于不含ctxAB基因的El Tor型霍乱弧菌中发现部分菌株含有RS基因同源序列，为此对这些菌株作CTXΦ基因组的进一步研究。

Based on the study of DNA isolation from different samples, this paper applied polymerase chain reaction and PCR-derived technology, and utilized many kinds of known samples and reference materials (such as soybean, maize, rice, potato, and pimiento, etc.) to establish the qualitative, half-quantitative, and quantitative GMO detection method: 1. We established a qualitative PCR screening method for CaMV 35S promoter and Tnos terminator. It must be taken into account that false positive results from screening method.

在DNA提取方法研究的基础上，本文应用聚合酶链式反应(polymerasechain reaction，PCR)及其衍生技术，利用大豆、玉米、水稻、马铃薯、甜椒等多种已知标准样品，建立了多种农作物和植物源性食品的GMO定性、半定量和定量检测方法： 1、在单基因定性检测技术方面，针对现有的商品化GMO中大多数含有花椰菜花叶病毒35S（CaMV 35S）启动子和胭脂碱合成酶基因终止子的特点，本文建立了CaMV 35S启动子和Tnos终止子的定性PCR筛选检测方法。

DETECTION OF PLASMODIUM VIVAX BY POLYMERASE CHAIN REACTION IN HAINAN .

应用聚合酶链反应在海南检测间日疟原虫的效果。

In 5 μM. pseudolaric acid B-treated HeLa cells, caspase-3 proenzyme decreased followed by the degradation of caspase-3 substrates, ICAD (inhibitor of caspase dependent DNase) and PARP poly-(ADP-ribose polymerase.

土槿皮乙酸5μM作用于HeLa细胞后，caspase-3的酶原被降解，caspase-3底物ICAD(inhibitor of caspase activated DNase)和PARPpoly-(ADP-ribosepolymerase降解，caspase-3的抑制剂可部分抑制土槿皮乙酸诱导的细胞死亡，表明土槿皮乙酸诱导HeLa细胞凋亡时激活了caspase-3。

Methods 112 blood samples of occupationally lead-exposed workers were selected from a puddling factory in the suburb of Chengdu. An assay based on the polymerase chain reaction-restriction fragment length polymorphism technique of restriction enzyme Bsm Ⅰ was used to detect at the same time.

整群抽取成都市郊区某炼铁厂接铅作业人员112人，抽取其静脉血进行血铅、血清钙和基因多态性测定，按基因型测定结果分组分别比较其血铅、血清钙的差异，并进行血铅和血清钙的相关性分析。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。