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Methods (1)Speciments of periphral blood were collected from 25 patients with JOAG ,their 10 relatives and 25 normal subjects. Polymerase chain reactionwas usd to analyze the sequence of MYOC gene consisting of all 3 exons and adjacent non-coding regions.

(1)应用聚合酶链反应方法，对25例开角型青光眼患者及其10例一级亲属和20例正常对照者的MYOC基因的3个外显子、部分内含子及部分启动子(7对引物)的各个片段进行扩增。

Methods: Genotypes of E-selectin were analyzed in 135 angina pectoris and 145 normal controls by polymerase chain reaction-restriction fragment length polymorphism. Plasma lipid levels were measured by biochemical technology.

应用聚合酶链反应-限制性片段长度多态性技术检测135例心绞痛患者和145例正常对照者E-selectin基因型，生化技术测定血脂水平。

Methods -3489C/T and 478C/T polymorphism were screened in 196 type 2 diabetic patients and 119 normal controls by polymerase chain reaction restriction fragment lenth polymorphism method.

运用聚合酶链反应-限制性片段长度多态性技术对196例T2DM患者和120例正常对照者的-3489C/T与478C/T多态性进行检测，并比较各组间基因型频率和等位基因频率以及相关临床资料。

The nucleoid size analysis, the comet assay and quantitative polymerase chain reaction.

细胞核大小分析，慧星检测法，PCR定量法。

The Nucleonics' HBV clinical candidate encodes four short hairpin RNA molecules, each under the control of an RNA polymerase III promoter.

该nucleonics的乙型肝炎临床候选四个编码短发夹核糖核酸分子每个控制下的一种核糖核酸聚合酶三子。

In recent years, more and more scientists presumed that RNA polymerase Ⅲ transcription might not occur in the nucleoplasm but in the nucleoli. Nevertheless, the possibility has not been proved directly.

近几年，有许多学者相继提出了RNA聚合酶Ⅲ有可能在核仁区域内发生转录的观点，但是这一观点至今还没有得到直接的实验证据的支持。

In recent years, more and more experiments indicated that RNA polymerase Ⅲ transcription might not occur in the nucleoplasm but in the nucleolus. Nevertheless, there is no direct experimental proof. In this dissertation, the plasmids bearing pol Ⅲ recognized promoters were transfected into the CHO cells by electroporation.

由于RNA聚合酶Ⅲ转录位点的研究存在特殊性，而传统的研究方法又存在一定的局限性，因此近几年人们对RNA聚合酶Ⅲ的转录发生在核质中这一结论提出了质疑，但至今尚缺乏直接的实验证据。

In this paper,techniques of reverse transcription polymerase chain reaction and rapid amplification of cDNA ends have been used to clone a gene,which encodes a general odorant binding protein,from the antennae of Loxostege sticticalis.

利用RT-PCR结合RACE技术克隆了编码草地螟触角中一种普通气味结合蛋白的基因。

Methods Polymerase chain reaction and restriction fragment length polymorphism was used to identify mdm2 genotypes. The Pearson Chi square test and Woolf statistic method were used to analyze the relative risk and 95% confidence interval in order to find the genetic association between polymorphism of mdm2 gene and symptoms and pathological types of NSCLC.

采用聚合酶链反应和限制性内切酶片段长度多态性方法检测88例NSCLC患者的mdm2基因型，并通过Pearson卡方检验及Woolf方法计算相对风险度及95%可信区间（95%CI），验证mdm2基因多态性与各临床症状及病理类型之间的关联性。

The aim of this study was to analyze the relative expression patterns of mRNA for IGFs in different development stage of preimplanted ovine embryos by using reverse transcription-polymerase chain reaction.

本研究主要在mRNA水平上检测了IGFs在绵羊植入前不同发育阶段胚胎中的表达规律。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。