查询词典 polymerase

Falciparum isolates prepared directly from 5 cases of cerebral malaria patients'blood in Mengla County, Yunnan Province and in Yingjiang County, Yunnan Province were used for polymerase chain reaction amplification and the two pairs of oligonucleotides for the highly conserved genes encoding the regions 12—17 in MAD20 merozoite surface protein 1(MSP1) of Papua New Guinea strain of P . falciparum were used as primers. The PCR products were digested with EcoRI and KpnI, respectively, and the generated fragment regions 16—17 were cloned into M13mp18 and M13mp19 vectors and their DNA were analyzed as the templates for DNA sequencing by the dideoxy chain-termination method.

应用多聚酶链反应技术对中国5 例脑型疟患者恶性疟原虫云南省勐腊县勐罕CMH/YN 分离株和云南省盈江县农场CYJ/YN 分离株基因组DNA 裂殖子表面蛋白1 (MSP1)第13—17 区基因进行扩增，将扩增产物分别经EcoRI 和KpnI 双酶切后，回收的MSP1 第16—17 区基因分子定向克隆M13mp18 和M13mp19 载体，按Sanger 双脱氧链终止法进行DNA 序列测定，并与MAD20、K1 和Wellcome 株原型基因进行同源性分析比较。

Methods Genomic DNA samples of two isolated Plasmodium falciparum isolate strains prepared directly from 5 cases of cerebral malaria patients′ blood in mengla County, Yunnan Province and in Yingjiang County, Yunnan Province were used for polymerase chain reaction amplification and the two pairs of oligonucleotides for the highly conserved genes encoding FC27 merozoite surface protein 2 (MSP2) of Papua New Guinea strain of Plasmodium falciparum were used as primers. The PCR products were digested with BamH1 and Hind Ⅲ respectively, and the generated fragment MSP2 were cloned into M13mp18 and M13mp19 vectors and their DNA was analyzed as the templates for DNA sequencing by the dideoxy chain-termination method.

应用多聚酶链反应对5例中国脑型疟患者恶性疟原虫云南省勐腊县勐罕分离株和云南省盈江县农场分离株基因组裂殖子表面蛋白2(MSP2)基因进行扩增，将扩增产物分别经BamHI和Hind Ⅲ双酶切后，回收的MSP2基因分子定向克隆M13mp18和M13mp19载体，按Sanger双脱氧链终止法进行DNA序列测定，并与恶性疟原虫株FC27、K1、IC1和CAMP株进行同源性分析比较。

Reverse transcriptase-polymerase chain reaction tests for eotaxin-1 messager RNA were performed in 5 cases of nasal polyps accompanied with chronic sinusitis and 2 cases with fungal ball sinusitis, and 2 cases of inferior turbinate mucosa.

并运用RT-PCR的方法检测5例慢性鼻窦炎鼻息肉﹑2例真菌球性鼻窦炎合并息肉和2例下鼻甲黏膜中Eotaxin-1mRNA的表达情况。

METHODS Microdissection technique with micromanipulator under microscope, as well as subsequent DNA extraction and polymerase chain reaction amplification methods, was employed.

通过光学显微镜直视下用显微操纵系统进行显微切割技术结合DNA提取和PCR方法，对在组织切片上获取病毒核内包涵体的来源进行分子生物学鉴定。

Methods MHC-DRB1, DQA1, DQB1 were examined by using polymerase chain reaction/sequence specific oligonuleotide probe methods in 19 patients with miliary tuberculosis, 36 with tuberculous menigitis,20 with primary tuberculosis, 28 with tuberculous pleurisy and 91 controls.

采用聚合酶链式反应-序列特异性寡核苷酸探针斑点杂交方法，测定原发性肺结核20例、结核性胸膜炎28例、粟粒型肺结核19例、结核性脑膜炎36例和91例正常对照组的MHC-DRB1，DQA1，DQB1等位基因。

A 25μL solution with genomic DNA 25ng/μL, MgCl_2 1.5mmol/L, dNTPs 0.1mmol/L, random primer 0.1μmol/L, 1U TaqDNA polymerase would be optimum for the amplification reaction. A high reproducibility was obtained with the optimized experiment conditions. The results of the genetic diversity among M. Mongolic, M. macrocopa and their s...

按照优化的RAPD条件进行实验，重现性良好。2种裸腹在盐胁迫条件下的种群遗传多样性分析表明，同种裸腹不同生存盐度之间的种群遗传相似度较高，分别为0.9134和0.9038；而多刺裸腹和蒙古裸腹两个种间的遗传相似度相对较低，约为0.5～0.6。

The I/D polymorphism of ACE was detected by polymerase chain reaction, and the CMA/B poly- morphism of Chymase gene was detected using PCR-restriction fragment length polymorphism.

运用聚合酶链反应检测 ACE基因的I/D多态性，运用限制性片段长度多态性技术检测Chymase基因的CMA/B多态性。

Coil O85 antigen contained 8 varieties of genes, including UDP-Nacetylglucosamine-2-epimerase gene (f1). UDP-galactopyranose mutase gene, glycosyl transferase genes (f3, f5, f6, f8), O-antigen transferase gene and O-antigen polymerase gene, in which two genes, wzx and wzy and 4 pairs of primers were identified to be specific to E.

发现8个开放阅读框架并确定功能，分别为：UDP-N-乙酰葡萄糖－2-异构酶基因，吡喃型UDP-半乳糖变位酶基因，糖基转移酶基因（orf3、orf5、orf6和orf8），O-抗原转运酶基因和O-抗原聚合酶基因。

Methods The effect of ginsenoside-Ro on murine splenocytes proliferation was studied using [3H] thymidine incorporation assay. Effects of ginsenodide-Ro on the production of cyrokines interleukin-2 (IL-2), interferon-γ and interleukin-4 (IL-4) from mutine splenocytes were detected by ELISA method. Effects of ginsenoside-Ro on mRNA level of TH1 cytokine IFN-γ and Th2 cytokine IL-4 were evaluated by reverse transcription polymerase chain reaction analysis.

方法[3H] TdR参人法检测人参皂有－Ro对小鼠脾淋巴细胞增殖的影响；酶联免疫吸附法检测人参皂苷－Ro对小鼠脾淋巴细胞产生细胞因子白介素－2、干扰素－γ和白介素-4的影响；逆转录聚合酶链式反应分析法研究人参皂苷-Ro对小鼠脾淋巴细胞引干扰素－Υ、白介素-4mRNA表达的影响。

Nested polymerase chain reaction for detection of Mycobacterium tuberculosis in clinical samples.

聚合酶链反应和DNA探针联合检测临床标本中结核杆菌的研究。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。