查询词典 polymerase

Objective To develop a nested-polymerase chain reaction to identify G and P genotypes of group A rotavirus.

目的用巢式聚合酶链反应对A组轮状病毒常见G、P基因型进行检测。

A general transcriptional factor for RNA polymerase II that binds to multiple promoter elements and specific transcription factors.

一种能与多个启动子元件和特异性转录因子结合的RNA聚合酶II通用转录因子。

Gene frequency/genet ; gene amplification/genet ; polymerase chain reaction/methods

基因频率／遗传学；基因扩增／遗传学；聚合酶链反应／方法学

The LV messenger ribonucleic acid levels of contractile and calcium regulatory genes were measured by quantitative real time polymerase chain reaction and normalized for glyceraldehyde 3-phosphate dehydrogenase.

采用定量实时聚合酶链反应测定心室收缩蛋白和钙调蛋白基因信使RNA水平，采用3-磷酸甘油醛脱氢酶标准化。

The rabbits' RNA was extracted from the lavage cells, and mRNA for TNFα was quantitated by reverse-transcription polymerase chain reaction using glyceraldehyde 3-phosphate dehydrogenase as an internal standard.

第二部分：12只表面活性物质缺乏兔，以CMV(n＝6)或PHY+PEEPi(n＝6)通气1h后，重复灌洗，提取灌洗液中的兔RNA，以GAPDH为内参照RT-PCR的方法来定量分析TNFαmRNA。

The authors tested the cervical excretions of 50 women by polymerase chain reaction forgonorrhea and compared the results with those tested by gonococcus cultures.

作者采用聚合酶链式反应诊断女性淋病50例，并将PCR与淋菌分离培养比较，结果50例宫颈分泌物PCR检测获得阳性35例，其中20例与常规分离培养法一致，另外15例PCR阳性而分离培养为阴性，此15例临床诊断为淋病。

Measles virus ; S191; hemagglutinin gene ; reverse transcription polymerase chain reaction ; sequence analysis ; molecular epidemiology

麻疹病毒； S191； H基因；逆转录—聚合酶链反应；序列分析；分子流行病学

A method of one-step reverse transcription polymerase chain reaction was developed to subtype hemagglutinin gene of Avian influenza virus.

建立一步法 RT-PCR 检测方法，对禽流感病毒(Avian influenza virus，AIV)的血凝素(Hemagglutinin，HA)分型进行了研究。

Calcium sulfate has been used for bone regenation in oral medicine. Osteopontin, a marker molecule, expressed by osteoblast, plays multiple roles in human body. However, the effect of calcium sulfate on osteopontin expressed in osteoblasts is unclear. In this study, U-2 osteosarcoma osteoblastic cells were cultured in 0, 0.5, 1, or 10 μM calcium sulfate hemihydrate. There was no effect in the proliferation of cells, suggesting that calcium sulfate up to 10 μM is biocompatible. The cells and conditioned media were collected for analyzing mRNA and protein levels of osteopontin by reverse-transcription polymerase chain reaction and Western blot, respectively.

硫酸钙用於口腔医学中的骨头再生已经有多年历史，骨桥素是一种被骨母细胞分泌且在人体扮演重要多重角色的标的分子，然而，硫酸钙在骨母细胞分泌硫酸钙上的影响仍未明朗，在本实验之中，培养U-2骨肉瘤类骨母细胞(U-2 osteosarcoma osteoblastic cells)於0、0.5、1、10 μM的硫酸钙之中，在细胞数目的复制上，四种硫酸钙浓度并无影响，显示出U-2骨肉瘤类骨母细胞培养於10 μM的硫酸钙仍是生物相容性的。

Nested reverse transcription polymerase chain reaction revealed that c-SPH mRNA is expressed most in tissues with the highest hemocyte abundance.

巢状反转录聚合酶连锁反应显示c-SPH mRNA的讯号在血球中最为强烈。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。