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On the other hand, flow cytometry was employed to detect the action of paclitaxel to cell cycle and apoptosis. Reverse transcription polymerase chain reaction is employed to detect the influence of paclitaxel on Survivin mRNA expression. The purpose of the study is to provide theoretical basis to carcinogenesis, progress, metastasis, angiogenesis and clinical chemotherapy of paclitaxel on TSCC.

采用流式细胞仪检测紫杉醇对舌癌细胞细胞周期的影响及应用逆转录聚合酶链反应(reverse transcription polymerase chain reac-tion，RT-PCR)检测紫杉醇对舌癌中Survivin mRNA的影响，为研究舌鳞癌的发生、发展机制，舌癌的转移及新生血管形成，及临床应用紫杉醇抗舌癌提供理论依据。

Target DNA fragments were amplified by polymerase chain reaction from the liver tissue of Columba livia using a pair of specific primers, and the amplified PCR products were cloned into pMD18-T vector.

利用特异引物，通过聚合酶链式反应(polymerase chain reaction， PCR)技术，从家鸽肝脏组织的总DNA中扩增到目的片段，并将扩增产物克隆到pMD18-T载体中，经菌落PCR与酶切鉴定、序列测定及序列分析。

Methods Polymerase chain reactionwas used to detect the HPV subgene in 25 specimens of laryngeal carcinoma and 6 normal larynges.

采用聚合酶链反应（polymerase chain reaction，PCR）技术对临床活体组织中HPV-E1的表达进行病毒病因学研究，所采用的公共产物代表HPV13个亚型。

Fluorescence in situ hybridization was carried out to confirm the integration of HBV DNA into male pronucleus and its replication with cell division in embryonic development.(Reverse transcriptase polymerase chain reaction,RT-PCR) and immunofluoresence assay were performed to observe the expression of the HBV gene in two-cell stage.

材料与方法：成熟雄鼠麻醉后双侧睾丸注射经脂质体DOSPER包裹的HBV质粒，手术后雄鼠与超排雌鼠合笼交配，用荧光原位杂变(Fluorescence in situ hybridization，FISH)分别检测单细胞胚和二细胞胚间期核中HBV DNA的存在与复制，用逆转录聚含酶链反应(Reverse transcriptase-polymerase chain reaction，RT-PCR)和免疫荧光方法检测HBV基因在二细胞胚胎中的表达。

KCR and HCD are erucic acid synthesis genes. In order to reveal the relationship between the synthesis of erusic acid and the quantitative expression of its functional genes, and to reveal the theoretic base of the breeding for high erucic acid content, the expression of KCR and HCD had been analyzed kinetically and quantitatively by using regular reverse transcription polymerase chain reaction and the internal standards of QuantumRNA18S during the growing period of high erucic acid content lines of Brassica Juncea.

研究应用常规RT-PCR(Reverse transcription polymerase chain reaction)的技术和QuantumRNA18S内标对芥菜型高芥酸油菜生育期内芥酸合成基因KCR、HCD基因的表达进行量化分析，了解芥酸的合成与功能基因量化表达的关系，为芥菜型油菜的高芥酸品种的选育提供理论依据。

In Picomaviridae, 3D protein was RNA dependent RNA polymerase, and had been applied indifferential diagnosis of FMDV infection, and other picornaviruses.

在PTV病毒中，3D蛋白是具有RNA依赖RNA聚合酶(RNA dependent RNA polymerase，RDRP)活性蛋白，能介导机体产生特异性的抗体。

SP-R mRNA in nasal mucosa, obtained from two groups, were used to do reverse transcriptive polymerase chain reaction and Southern blot.

以β-肌动蛋白作内标准，利用逆转录-多聚酶链反应(reverse transcriptive polymerase chain reaction， RT-PCR)，对变应性鼻炎模型组和对照组大鼠鼻粘膜SP-R mRNA进行了定量研究。

In this study,one pair of specific primer for mature chicken interleukin-18(ChIL-18) cDNA was designed and synthesized according to the previously published gene sequence of ChIL-18.The full length cDNA gene of ChIL-18 encoding mature active protein was amplified from LPS–stimulated MDCC-MSB1 cells by Reverse Transcription-Polymerase Chain Reaction.Then it was cloned into pMD18-T vector. Sequencing analysis showed that the nucleotide sequence of this ChIL-18 mature protein gene was 5l0bp including the stop coden and the same as the published ChIL-18 cDNA sequence by Schneider K.

本研究根据已发表的ChIL-18成熟蛋白(mature ChIL-18，mChIL-18)的cDNA基因序列，设计一对特异性引物，应用反转录-聚合酶链式反应(reverse transcription-polymerase chain reaction，RT-PCR)技术从脂多糖刺激10小时活化的马立克氏病成淋巴细胞样细胞系MDCC-MSB1的cDNA中扩增出编码鸡IL-18成熟活性蛋白的全长基因，对扩增片段进行T-A克隆，经PCR、酶切鉴定及测序验证，成功获得ChIL-18成熟蛋白完整基因的克隆。

Based on establishment of the human macrophage-derived foam cell model, the expression of Kv1.3 channel was investigated by immunocytochemical staining, reverse transcription-polymerase chain raction and Western blot. Furthermore, the effects of rMargatoxin, a Kv 1.3 channel-specific inhibitor, on cholesterol metabolism in macrophages incepting oxidized low density lipoprotein were studied.

在建立人巨噬细胞源性泡沫细胞模型的基础上，采用免疫细胞化学染色法、逆转录聚合酶链反应(transcription-polymerase chain raction，RT-PCR)及Western blot，研究Kv1.3通道的表达，并观察其特异性阻断剂--rMargatoxin对摄取氧化修饰低密度脂蛋白的巨噬细胞胆固醇代谢的影响。

Methods Using polymerase chain reaction-restriction fragment length polymorphism method, we detected 63 samples taken from 63 cases (including 36 invasive squamous carcinoma, 7 carcinoma in situ, 20 papillomas) for 248 point mutations in p53 gene.

采用聚合酶链反应――限制性片段长度多态性（polymerase chain reaction-restriction fragment length polymorphisms，PCR-RFLP）方法，对63例患者的送检标本（包括36例浸润性鳞状细胞癌，7例原位癌，20例乳头状瘤）中p53基因248位点的突变进行检测。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。