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A new monomer,4-hexanoxyl-4′-undecylene-[10]-acyloxyazobenzene was synthesized using the main materials:phenol,underlenic acid,1-bromo- n -hexane, p -nitrophenol,thionyl chloride,by the substitution,reduction,couping and diazotization methods.A new polymer was prepared by hydrosilation reaction of poly (methylhy-drosiloxane) with the monomer.

以苯酚、十一烯酸、正溴己烷、对硝基苯酚和二氯亚砜为主要原料,通过取代、还原、重氮化和偶合等反应,合成了一种新的液晶单体 4-己氧基-4′-十一烯-[10]-酰氧基偶氮苯,并与聚通过硅氢加成生成一种新的高分子液晶。

Fig.4 GFAP positive cells after plating clones onto poly-L-lysine coverslip 7 days in vitro(SABC-Cy3 immuno-fluocence).

图4 在多聚赖氨酸包被玻片上诱导分化7d后克隆细胞呈GFAP阳性(SABC-Cy3免疫荧光)。

Fig.5 CNP positive cells after plating clones onto poly-L-lysine coverslip 7 days in vitro(SABC-Cy3 immuno-fluorescence).

图5 在多聚赖氨酸包被玻片上诱导分化7d后克隆细胞呈CNP阳性(SABC-Cy3免疫荧光)。

Methods The neural stem cells derived from 12-12.5 day embryonic rat cortex were planted in suspended condition (three-dimension) and on coverslip precoated by poly-L-lysine.

取孕12~12.5天SD大鼠胚胎的大脑皮层细胞体外培养,建立神经干细胞模型。

The BTSCs attached to poly-L-lysine-coated coverslips and differentiated when the serum-supplemented medium was added.

在含血清培养基中脑肿瘤干细胞发生贴壁分化,产生具有多种细胞形态的分化细胞。

After calculating the concentration by counting board, cell suspension was cultured on the coverslips coated poly-L-lysine at 1×109/L, then the coverslips were paved in the bottom of 35 mm culture dish for 3 weeks.

经12 h+ 24 h两次差速培养后,吸出细胞上悬液,计数板计算浓度后,按1×109 L-1浓度重新种植于载有多聚赖氨酸包被盖玻片的35 mm培养皿中继续培养3周。

Dissociated cells were plated onto poly-d-lysine-coated coverslips and propagated in medium containing recombined Wnt3a-adenovirus. Plenty of Nurr1 were detected by RT-PCR after 3 days.

将神经干细胞分为4组,对照组、抗坏血酸诱导组、Wnt3a重组腺病毒诱导组(Wnt3a组)以及Wnt3a重组腺病毒加抗坏血酸诱导组(Wnt3a+AA组)。

Using poly(benzyl-ether) dendrons with thiol functionalities and CNTs/CdS core-shell nanowires as reactants, using chloroform as solvent, through adjusting the temperature and time, soluble CNTs/CdS core-shell nanowires have been obtained.

采用带巯基的聚苯甲醚树枝状分子和CNTs/CdS核壳纳米线在有机溶剂中作用,通过调节反应时间和温度,成功制备了可溶性CNTs/CdS核壳纳米线结构。

Based on the previous experiments, we choose CNTs/CdS core-shell nanowires as inorganic materials and poly(benzyl-ether) dendrons with thiol functionalities as organic solubilizer. The solubility of CNTs/CdS core-shell nanowires in normal solvents was expected to improve through chemical interactions between core-shell nanowires and solubilizer. The enhanced solubility can improve facility in processability. It also provides a new application method in nanomaterials.

本论文在课题组原有工作的基础上,以制备可溶、易加工的有机-无机复合纳米材料为应用目标,选择具有自己课题组研究特色的CNTs/CdS核壳纳米线结构作为无机材料,选择带巯基的聚苯甲醚树枝状分子作为有机增溶剂,通过带巯基的聚苯甲醚树枝状分子对CNTs/CdS核壳纳米线的化学作用,改善了核壳纳米线在常用有机溶剂中的溶解性能,为材料的湿法加工提供了基础,也为类似纳米材料的应用开拓了一种新的方法。

The morphologies formed were characterized by SEM and DLS methods, and spherical particles with various sizes were observed.5. To obtain a amphiphilic dumbbell-shaped copolymer, we successfully synthesized dendritic-linear-dendritic triblock copolymers composed of linear polystyrene and poly dendrons.

树枝状共聚物最外层PSt-PNAS链段利用乙二胺和NAS活性酯的取代反应交联,同时,中间的聚丙交酯段被降解,得到纳米空心球,用SEM和DLS进行观测和表征。5。

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