查询词典 pokeweed

Milkweed of the eastern United States with leaves resembling those of pokeweed.

生长于美国东部的马利筋，与美国商陆的叶子相似。

Many studies revealed that pokeweed antiviral protein can control plant virus effectively.

许多研究发现美洲商陆抗病毒蛋白(pokeweed antiviral protein，PAP)对植物病毒具有良好的控制效果。

SDS-PAGE results showed that as to Mut+ recombinant highest yield was obtained after 4 days inducing and with the culturetime prolonged it reduced. Pokeweed antiviral protein gene expressed well when methanol concentration reached 10g/L. Pokeweed antiviral protein obtained high yield in thin acidic culture medium (pH6.0-6.4) and its quantity in total mass of secrete protein exceeded 30%.

SDS-PAGE分析结果表明，Mut~+组菌株在甲醇诱导第四天后PAP在培养液中积累量达到最高水平，延长培养时间会导致产量下降；在10g/L的甲醇浓度诱导下，PAP的表达量达到最高；培养基pH值在偏酸性条件下(6.0-6.4)PAP的表达量都维持在较高的水平。

There are some isoforms of PAPs showing inhibitory ability to virus infection in various organs and at different stages of development. Three different species of the protein can be isolated from leaves of pokeweed. Proteins were precipitated with 50-80% ammonium sulfate from leaves in spring, summer and autumn respectively. Proteins were purified on SP cation-exchange then on MQ anion-exchange and finally on MS cation-exchange or on MQ anion-exchange. We found that some new pokeweed antiviral proteins separated from these leaves were different from PAP, PAPII and PAPIII.

对美洲商陆春季、夏季、秋季采集的叶片，用50-80％饱和度硫酸铵沉淀出来的蛋白样品，经过SP阳离子柱后，上MQ阴离子柱，然后把活性蛋白峰再上MS阳离子柱或MQ阴离子柱分离，从春季叶片中分离出四个不同的抗病毒蛋白峰；从夏季叶片中分离出二个不同的抗病毒蛋白峰，这两个蛋白峰与春季叶片中分离出的活性蛋白峰都能重叠上；但从秋季叶片中只分离出来一个抗病毒蛋白峰，它和春季、夏季叶片中分离出的活性蛋白峰都重叠不上。

There are some isoforms of PAPs showing inhibitory ability to virus infection in various organs and at different stages of development. Three different species of the protein can be isolated from leaves of pokeweed (PAP, PAPⅡ and PAPⅢ). Proteins were precipitated with 50-80% ammonium sulfate from leaves in spring, summer and autumn respectively. Proteins were purified on SP cation-exchange then on MQ anion-exchange and finally on MS cation-exchange or on MQ anion-exchange. We found that some new pokeweed antiviral proteins separated from these leaves were different from PAP, PAPⅡ and PAPⅢ.

对美洲商陆春季、夏季、秋季采集的叶片，用50-80％饱和度硫酸铵沉淀出来的蛋白样品，经过SP阳离子柱后，上MQ阴离子柱，然后把活性蛋白峰再上MS阳离子柱或MQ阴离子柱分离，从春季叶片中分离出四个不同的抗病毒蛋白峰；从夏季叶片中分离出二个不同的抗病毒蛋白峰，这两个蛋白峰与春季叶片中分离出的活性蛋白峰都能重叠上；但从秋季叶片中只分离出来一个抗病毒蛋白峰，它和春季、夏季叶片中分离出的活性蛋白峰都重叠不上。

The total RNA was isolated from pokeweed leaves using the method of guanidine isothiocyante and used as template for amplification of the deleted mutant pokeweed antiviral protein gene by RT-PCR and then the amplified fragment was cloned into secreted expression pPIC9K vector to form pPIC9K-p, then the vector was transferred into Pachia pastoris GS115 strain.

从美洲商陆叶片中通过异硫氰酸胍法提取出了总RNA，经RT-PCR扩增出缺失突变型抗病毒蛋白PAP基因，将该基因克隆于分泌型真核表达载体pPIC9K，然后导入毕赤酵母菌株GS115细胞。

Pokeweed antiviral protein, a 29-kD protein isolated from Phytolacca americana, inhibits translation by catalytically removing a specific adenine residue from the large rRNA of the 60s subunit of eukaryotic ribosomes. In addition to its ribosome-inactivating ability, PAP has potent antiviral activity against many plants and animal viruses.

美洲商陆抗病毒蛋白(pokeweed antiviral proteins，PAPs)，是在美洲商陆的不同组织或不同生长阶段生成的一类具酶功能的单链核糖体失活蛋白(ribosome-inactivating proteins，RIP)，是天然的广谱抗病毒生物活性物资，能抗多种植物和动物病毒。

Transgenic tobacco plants were obtained through screening with kanamycin. The transgenic tobacco plants could delay TMV infection for about 25 days compared with non-transgenic tobacco plants. Pokeweed antiviral protein Ⅱ is expressed with high level in summer leaves. The expression of PAPⅡ is regulated by season. The total RNA was extracted from pokeweed (Phytolacca americana L.) leaves in summer using the method of TRIzol and used as template to amplify the PAPⅡ gene by RT-PCR and then the gene was cloned into E. coli expression vector and secreted expression pPIC9K vector. The two vectors with PAPⅡ gene were then transferred into E. coli strain BL21 (DE3)-plysS and Pachia pastoris GS115 strain respectively. The specific protein was produced induced by IPTG and methanol.

由于美洲商陆抗病毒蛋白Ⅱ是一个受季节调控表达的蛋白，本实验以美洲商陆夏季叶片为材料，通过对其总RNA的提取、反转录、并用PAPⅡ的特异引物进行PCR扩增，对PAPⅡ进行了基因克隆、序列分析，结果扩增出来的PAPⅡ基因与已经报道的序列同源性是99.9％，然后将该基因构建到原核、真核表达载体上，分别转化了大肠杆菌和毕赤酵母并对它们进行了诱导表达，在两个表达系统中均获得了有活性的PAPⅡ表达蛋白，体外生物测定表明表达的蛋白均具有抑制病毒的活性，PAPⅡ基因在酵母中还没有表达的报道，这为获得具活性PAPⅡ蛋白提供了一种简便可行的方法。

The transgenic tobacco plants could delay TMV infection for about 25 days compared with non-transgenic tobacco plants.Pokeweed antiviral protein II is expressed with high level in summer leaves. The expression of PAPII is regulated by season. The total RNA was extracted from pokeweed (Phytolacca americana L.) leaves in summer using the method of TRIzol and used as template to amplify the PAPII gene by RT-PCR and then the gene was cloned into E.coli expression vector and secreted expression pPIC9K vector. The two vectors with PAPII gene were then transferred into E.coli strain BL21 (DE3)-plysS and Pachia pastor is GS115 strain respectively. The specific protein was produced induced by IPTG and methanol.

由于美洲商陆抗病毒蛋白Ⅱ是一个受季节调控表达的蛋白，本实验以美洲商陆夏季叶片为材料，通过对其总RNA的提取、反转录、并用PAPⅡ的特异引物进行PCR扩增，对PAPⅡ进行了基因克隆、序列分析，结果扩增出来的PAPⅡ基因与已经报道的序列同源性是99.9％，然后将该基因构建到原核、真核表达载体上，分别转化了大肠杆菌和毕赤酵母并对它们进行了诱导表达，在两个表达系统中均获得了有活性的PAPⅡ表达蛋白，体外生物测定表明表达的蛋白均具有抑制病毒的活性，PAPⅡ基因在酵母中还没有表达的报道，这为获得具活性PAPⅡ蛋白提供了一种简便可行的方法。

Pokeweed antiviral protein, a 29-kD protein isolated from Phytolacca americana, inhibits translation by catalytically removing a specific adenine residue from the large rRNA of the 60s subunit of eukaryotic ribosomes. In addition to its ribosome-inactivating ability, PAP has potent antiviral activity against many plants and animal viruses.

美洲商陆抗病毒蛋白(pokeweed antiviral proteins，PAPs)，是在美洲商陆的不同组织或不同生长阶段生成的一类具功能的单链核糖体失活蛋白(ribosome-inactivating proteins，RIP)，是天然的广谱抗病毒生物活性物资，能抗多种植物和动物病毒。

In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

。但是，在否定句或疑问句里，这种带有"do"的方法表达的效果却没有什么强调的意思。

Go down on one's knees;kneel down

屈膝跪下。。。下跪祈祷