查询词典 plasmids

MethodsThe D. bacteria - E. coli shuttle expression vector pZT17 was constructed based on plasmids of pUE30, pGBM5 and pKatCAT. Then pZT17 with lux+ from Photinus pyralis was used to transform into D. grandis and E.coli. The recombinant strains were induced separately.

以质粒pUE30、pGBM5及pKatCAT为基础，构建抗辐射菌属一大肠杆菌间的穿梭载体，将groEL启动子和荧光素酶基因lux+插入到构建的穿梭载体中得到穿梭表达载体，并将该载体转化大肠杆菌诱导荧光素酶基因的表达。

Objective: The prospective investigation of lymph secluding metastasis of T2-4 N0-phase in head-neck squamous cell carcinoma of small sample, and made prepare for constructiving plasmids.

目的：通过检测T2~4 N0头颈部鳞状细胞癌培养的肿瘤细胞及新鲜活检组织中VEGF-A、C及VEGFR-3的表达情况及构建质粒，探讨是否可以通过组织小样本前瞻性研究了解T2~4 N0患者淋巴管受累及局部淋巴转移情况并为制备VEGFR-3抗体作好准备。

The late phase expression cassette suited for adenovirus vectors was also constructed. The HBV S gene, HBV preS2+S gene and the late phase expression cassette +HBV S genes were respectively cloned into the Ad5 vector downstream of the E3 promoter. Semiconfluent monolayers of 293 cells were cotransfected with the above constructed plasmids and Ad5 DNA EcoRI A fragment. The progeny adenoviruses named rAd5S, rAd5MS, rAd5S2S were harvested.

通过共转染优化实验，建立了脂质体转染腺病毒的有效方法，并构建了适合腺病毒载体的晚期表达盒，进一步将乙型肝炎表面抗原基因、HBV preS2+S、腺病毒晚期表达盒+HBV S基因插入E3区，与EcoRI消化的Ad5 DNA A片段共转染细胞，并获得重组腺病毒，相应命名为：rAd5S、rAd5MS、rAd5S2S。

As for the molecular genetics of plasmids in thermophilic bacteria, only preliminary observation of very few phenomena has been reported so far.

在高温细菌质粒的分子遗传学方面，已发表的少量文献仅限于对个别现象所作的初步描述。

On other hand, specific fragments containing thermostable DNA polymerase gene were amplified from the genomic DNA of some strains of thermophilic bacteria isolated from Yunnan hot springs, and recombinant plasmids were constructed.

同时，我们还利用特异性的热稳定DNA聚合酶基因的扩增引物，从生长在云南温泉的嗜热菌总DNA中筛选出了能被此类引物扩增的特异性片段，并构建了相应的重组质粒。

Subcloning technique was performed to construct the expression plasmids of EGFP-fused TIAR full length and truncated mutants.

结果细胞免疫荧光结果显示在SMCC-7721细胞中TIAR主要浓集于细胞核，细胞浆也有少量弥散分布。

Secondly, the genomic DNA is extracted from Zostera marina L. and then amplified by PCR with two oligonucleotide primers synthesized to obtain the partial nucleotide sequences of the Na~+/H~+ antiporter gene. The Na~+/H~+ antiporter gene clone was obtained after a series of manipulation: purificatioin of PCR product, ligation and transformation of recombinant plasmids, blue-white selection and so on. DNA sequence analysis shows that its DNA sequence is highly homologous with the genomic DNA sequence of E.

其次，通过提取大叶藻基因组DNA，根据设计的简并性引物进行PCR扩增，纯化PCR产物，连接，重组质粒转化，蓝白斑筛选等操作，并测序，提交测定序列到NCBI进行BLASTN，结果表明：该序列与大肠杆菌的基因组序列相似性较高，而与其它物种的Na~+/H~+逆向转运蛋白基因没有相似性。

Therefore, it is important to ask whether both Oct-2 and NF-κB participate the MEK/ERK dependent G-CSF expression. G-CSFp-(-289~+25)-Luc, CSF-box-deleted G-CSFp-(-145~+25)-Luc, or octamer-mutated Oct-mut-G-CSFp-(-289~+25)-Luc reporter plasmids were used to investigate the importance of CSF-box and octamer in response to LPS.

在G-CSF的启动子上有两段已被发现对其活性相当重要的序列：CSF-box及octamer，根据过去研究及本实验室未发表的实验结果，此两段序列分别由NF-κB以及Oct-2所结合；於是我们推测MEK/ERK讯息传递路径的下游可能透过NF-κB及Oct-2来调控G-CSF表现。

Objective To define the method of acclimating isolating and screening of dominant bacteria for phthalic acid esters biodegradation and study their plasmids.

目的确定高效酞酸酯降解菌的驯化与筛选方法，并对PAEs降解性基因功能片段进行初步研究。

The P32 gene of capripoxvirus were amplified by PCR.The product of PCR was cloned to the plasmid pGEM-T-Easy.Then the P32 gene was disparted to three parts.Three parts of P32 gene were inserted into the expressed plasmids pGEX-4T-1, which were identified by PCR and restriction enzyme analysis.

本研究利用PCR方法对羊痘病毒P32蛋白基因扩增，将回收PCR产物克隆到pGEM-T-Easy载体中，然后将P32蛋白基因分成3段，分别插入原核表达载体pGEX-4T-1中，经PCR和酶切鉴定，证明成功构建了重组表达载体pGEX-4T-1-N2/3、pGEX-4T-1-N1/3、pGEX-4T-1-M。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！