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The recombinant plasmids constructed successfully were then transfected into HepG2 cells and expressions of interceptive AIF were detected by RT-PCR and Western blot.

成功检测到截断型在转录与翻译水平上的表达。

The eukaryotic expression plasmids were used as genetic vaccines to immunized intramuscularly experimental animals and post-weaning piglets.

同时还研究证明猪白细胞介素2和6基因对猪伪狂犬病核酸疫苗的免疫增效作用。

Then, we cloned the lipA and its partner gene lipB into the vector pUCPCM and pBBR22b both or separately. Therefore, we got 7 expression plasmids pBBR22blipAB, pBBR22blipA, pUCPCMlipAB, pUCPCMlipA, pUCPCMΔlipAlipB, pUCPCMΔlipA, pUCPCMΔlipB, and then electroporated them into B.

接着把lipA和它的伴侣基因lipB单独或全部克隆到载体pUCPCM和pBBR22b上，构建出pBBR22blipAB、pBBR22blipA、pUCPCMlipAB、pUCPCMlipA、pUCPCMΔlipAlipB、pUCPCMΔlipA、pUCPCMΔlipB七种表达质粒，通过电转化将上述表达质粒转化到含T7 RNAP的B。

Objective: To investigate the antibiotic resistance and the epidemic situation of penicillin and tetracycline resistance plasmids of Neisseria gonorrhoeae in Changsha area.

目的：了解长沙地区淋病奈瑟菌对抗生素的敏感性以及耐青霉素和耐四环素性质粒的流行情况。

One could nodulate the soybean cultivars tested and showed wider host range, the other only nodulate limited soybean cultivars. Plasmid could not be detected from twenty-three slow-growing strains and twenty-nine fast-growing strains were found containing 1-4 plasmids by Echardts method.

质粒快速检测结果表明：慢生型大豆根瘤菌均不含有质粒，快生型大豆根瘤菌均有1-4个质粒，质粒类型与宿主亲和性也有一定的相关性。

Methods: The human KDR promoter (-225 bp~+127 bp) was cloned by PCR. Subsequently, a recombinant adenoviral plasmid carrying KDR promoter for the HSV-tk gene was constructed with the AdEasy system. As a control, the plasmid pAdCMV-tk carrying CMV promoter for the HSV-tk gene was also constructed. 293 packaging cells were transfected by both newly-constructed plasmids and the infectious viruses AdKDR-tk and AdCMV-tk were generated. Then the KDR-producing cells and the KDR nonproducing cells (HepG2) were infected with AdKDR-tkand AdCMV-tk, followed by ganciclovir administration.

应用PCR克隆出人KDR基因启动子序列－225bp~+127bp，以AdEasy system为载体，构建携带受KDR启动子或CMV启动子调控tk基因表达的2种重组腺病毒质粒pAdKDR-tk和pAdCMV-tk，在293细胞中包装、扩增后，体外感染表达KDR的脐静脉血管内皮细胞系HUVEC和不表达KDR的肝癌细胞系HepG2，并给予不同浓度的丙氧鸟苷处理，5d后收集存活细胞并计数。

After being digested with EcoR I and Xho I, they were inserted to the vectors pGEX-4T-l orientally, and then transformed into E.coli BL21(DE3) cells. The monoclone were selected and identified by means of restriction analysis, PCR and sequencing. As the result, the two recombinant plasmids with the different DNA fragements of the PrP gene were constructed and designated as pMY01[Ov rPrP(23-244)] and pMY02[Ov rPrP(91 -244)], respectively.

挑选单个菌落，提取重组质粒，经酶切、PCR扩增后琼脂糖凝胶电泳分析和测序鉴定，成功地构建了2种绵羊重组朊蛋白的表达质粒pMY01[OvrPrP(23～244)]和pMYO2[Ov rPrP(91～244)]。

For example, it is a pathogenic bacterium and has endotoxins; it secretes protein into the periplasm and often into inclusion bodies. Bacillus subtilis is an attractive host for the production of heterologous secretory proteins. Many genes has succeed expressed in Bacillus subtilis, and the plasmid vectors for Bacillus subtilis are now taken from other genus bacterium, such as Staphylococcus aureus or lactococcus lactis and so on. As it is clear to known that these plasmids are far from optimal for Bacillus subtilis, because of low efficiencies of cloning and high levels of replicating instability.

枯草芽胞杆菌是目前原核表达系统中分泌表达外源蛋白较理想的宿主，近几年已在枯草芽胞杆菌中成功表达了多种基因，但大多都应用来源于金黄色葡萄球菌、乳酸杆菌等的载体来表达外源基因，由于其在枯草芽胞杆菌中存在分离复制的不稳定及外源基因的克隆表达效率低的现象，故都不是枯草芽胞杆菌的最佳选择，还有待改进。

Results Multicopy recombinant plasmids pDGXHP2.0-2M Fα-HBsAg and pDGXHP2.0-4MFα-HBsAg were constructed. Three recombinant Hansenula polymorpha strains for secretory expression of HBsAg were screened.

结果 构建了HBsAg汉逊酵母多拷贝表达质粒pDGXHP2.0-2MFα-HBsAg和pDGXHP2.0-4MFα-HBsAg，筛选获得了分泌表达HBsAg的汉逊酵母HP/2MFα-HBsAg和HP/4MFα-HBsAg。

The tested parameters included: the effect of preculture duration for explants, the type of microcarriers, density of microcarrier particles used for DNA preparation, the effects of osmotic treatments, and plasmids with various constructions. Parameters of biolistic device including the flight distance of microcarriers (6 and 9 cm) and macrocarriers (4, 8, and 12 mm) were investigated.

评估之因子包括：培植体预培养天数、微载体种类及用量、高渗透压处理及转殖质体对转殖之影响；基因鎗测试之条件有：微载体至射击目標之飞行距离(6、9 cm)，大载片至挡片之距离（4、8及12 mm）。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！