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After 72h induced by Progesterone and Glucocorticoid as well as Insuline, biological activities of IL-2 were assayed with morphological docimasia method. The results showed that the plasmids of pcDNA3-QVP-IL2 and pcDNA3-IL2 could express IL-2, and the IL-2 could augment T cell proliferation.

结果显示，转染细胞均可表达IL-2，且所表达的IL-2有促进T淋巴细胞转化和淋巴母细胞成熟的活性；转染pcDNA3-OVP-IL2的输卵管上皮细胞表达产物在1：256稀释水平下仍具有促进T淋巴细胞转化和淋巴母细胞成熟的活性。

In this research work, the technology of microbial conversion was studied in order to establish an environment friendly method for the production of doxorubicin. A doxA gene encoding Daunorubicin C-14 hydroxylase was cloned from a daunorubicin-producing strain Streptomyces coeruleorubidus SIPI 1482. Some plasmids for the expression of doxA gene were constructed and transformed into S.

本研究对微生物转化法替代现有工艺生产阿霉素进行了探索，从柔红霉素产生菌天蓝淡红链霉菌SIPI 1482中克隆了柔红霉素C-14羟化酶基因，构建了doxA基因的链霉菌表达质粒，导入变铅青链霉菌TK24中获得了柔红霉素转化基因工程菌，并研究了doxA基因在工程菌中的表达，最后还对工程菌转化柔红霉素生成阿霉素的发酵工艺进行了初步研究。

Five plasmids for the expression of doxA gene, which were designated as pYG57, pYG502, pYG503, pYG505 and pYG506 were constructed so that the cloned doxA gene were under the control of either promoter of melanin gene or erythromycin resistant gene or their tandem promoters, and a fd terminator downstream.

构建了五个doxA基因链霉菌表达质粒pYG57、pYG502、pYG503、pYG505和pYG506，使得doxA基因在上游的Pmel启动子、红霉素抗性基因启动子或者两者的串联启动子，以及下游的fd终止子控制之下。

Linear and circular plasmids were investigated among the 44 strains of the rice plant endophytic Streptomyces.

以广东番禺和五山地区水稻植株中分离到的内生链霉菌为对象，调查可能存在的内源质粒。

Existing plasmids and inherited materials are used to construct expression vector pZMW which could be integrated into S. erythraea chromosome and multi-copy expression vector pZM.

本实验室利用现有的质粒和遗传材料，构建了糖多孢红霉菌染色体整合型表达载体pZMW和游离型多拷贝表达载体pZM。

Furthermore, K562 cells transfected by lentivirus plasmids were induced into the erythropoietic cells and the erythropoietic differentiation of K562 cells were examined by benzidine staining, immunocytochemistry and RT-PCR.

进一步对SOCS-3基因沉默后的K562细胞进行了诱导分化，并采用联苯胺染色法检测K562细胞向红系分化比例变化，免疫荧光染色检测细胞表面抗原的变化，RT-PCR 检测造血相关基因的变化。

PGAD424 that has the activation domain of yeast'transcript activation factor GAL4 is a kind of shuttle vector that could replicate in both E. coli and yeast. One-sixth recombinant plasmids of this library could express fusion protein of GAL4-AD and proteins from Heliothis armigera.

通过在合成cDNA第一链时参入α〓PdCTP，显示出合成的cDNA为6.0Kb以下的连续区带，测得文库的滴度为1.5×10〓，可以代表亲本细胞的全部编码蛋白质的基因序列，即该文库具有代表性。

All of them included hAAT gene as the reporter gene.BALB/c mice were transfected with the plasmids by hydrodynamical transfection technology.

但该技术的缺点是转染基因多瞬时表达，外源基因在体内高效表达的时间通常不超过1周，限制了该技术的广泛应用。

The recombinant plasmids were analyzed by sequencing and the one containing the correct fragment was transfected into K562 cells. The condition of idiotypic protein expression was tested by Western-Blot.

将经测序鉴定正确的重组质粒转染K562细胞，Western-Blot等方法检测其蛋白表达的情况。

The recombinant plasmids, pIRES-Jurkat Vβ8 and pIRES-Molt4 Vβ2①/②, containing idiotypic TCR Vβ8 frgment of Jurkat cell strain or idiotypic TCR Vβ2 frgments of the Molt4 cell strain were developed.

构建了Jurkat细胞独特型TCR Vβ8 DNA重组质粒pIRES-Jurkat Vβ8和Molt4细胞独特型TCR Vβ2 DNA重组质粒pIRES-MOLT4 Vβ2①/②。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！