查询词典 plasmids

The recombinant expression plasmids were constructedby fusing theHNP-coding fragment with intein and only the amidation of HNP-2 might beobserved.

通过对HNP-1，HNP-2和HNP-3的研究，在对HNP-2的酰胺化修饰上已获得一定进展。

The recombinant expression plasmids were constructed by fusing the HNP-coding fragment with intein and the amidation of HNP-2 might he observed.

本文采用了一种新的酰胺化方法，即将intein与HNP-2融合表达，intein对目的短肽进行酰胺化修饰。

Objective To construct prokaryotic recombinant plasmids of transcriptional co-activator gene of Clonorchis sinen sis, express and purify the recombinant protein and analyze its biological function.

摘要目的构建华支睾吸虫成虫转录辅激活因子(transcriptionalcoactivator ，TC)基因原核重组质粒，进行原核表达、鉴定及生物功能分析。

The GPA1 gene was obtained via PCR amplification and was cloned in the two-hybrid DNA binding domain vector pGBKT7 The combinant plasmid was designated as pGBKT7-Ga.β-galactosidase assay indicatedthat Got did not have the property of self-activation. After pGBKT7-Ga was transformed to yeast pJ69-4A, we transformed Arabidopsis vegetative tissue two-hybrid cDNA library plasmids to yeast pJ69-4A containing pGBKT7-Ga.

通过PCR扩增得到GPA1基因并将其克隆到双杂交DNA结合域载体pGBKT7中，得到的重组质粒命名为pGBKT7-Gα，β—半乳糖苷酶活性鉴定表明Gα不具有自激活特性，将其转化到酵母菌pJ69-4A中，再以此为受体菌转化拟南芥绿色营养组织cDNA文库质粒。

The development of research strategies and novel molecule methods in recent years have made a more careful analysis of HGT possible under natural and artificial conditions. Furthermore, horizontal transfer of catabolic gene in environment enhances biodegradation of recalcitrant organic compounds. Particularly, the delivery of catabolic gene information of conjugative plasmids to bacterial population in situ has great potential in bioenhancement.

近年发展起来的研究策略和新的分子生物学方法使人们能对自然和人工模拟状态下的水平基因转移进行更精细的分析，降解基因在环境中的水平传播促进了难降解有机物的生物降解，尤其是携带降解基因的接合质粒向原位细菌种群中的转移在生物强化中具有巨大的潜力。

The blocking mechanism takes advantage of the fact that a small sequence of this CRISPR locus matches staphylococcal conjugative plasmids, including those that confer antibiotic resistance in MRSA strains.

这一位点的阻断机理是利用了以下事实：CRISPR位点上的一小段序列与金黄葡菌中包括引起抗生素抗性质粒在内的结合质粒中的序列互补。

Coli. The shuttle co-expression plasmids were introduced into filamentous cyanobacterium-Anabaena sp. PCC 7942 (or 7120) by a triparental conjugative transfer method.

采用双顺反子体系在pTrc载体中成功的实现了A、B和Bc亚基的单独活性表达及A、B亚基共表达。

Elimination of conjugative drug resistance plasmids and nonconjugative drug resistanceplasmids from 20 and 19 Shigella strains respectively by norfloxacin and berberine in vitro were analysed.

用氟哌酸和黄连素对20株携有接合传递耐药性质粒及19株携有非接合传递耐药性质粒志贺氏菌进行体外消除研究。结果显示，可被消除的耐药性质粒大多数在24小时内被消除。

The range of MIC90 was-10 μg/ml. The susceptibility rates of the strains to other 10 agents were nearly or equal to 0%. Different plasmid profile strains had almost the same antimicrobial susceptibilities. 25 strains (including 18 strains harboring 34-80×106 plasmids) selectively examined to have no self-transmissible conjugative plasmid.

88.6%，MIC90为-10 μg/ml），对卡那霉素等其余10种药物的敏感率很低或不敏感，不同质粒谱菌株对药物的敏感性几乎相同，对包括 18株含34-80×106质粒在内的 25株菌进行接合转移试验，未检出传递性耐药性质粒。

Methods Agarose gel electrophoresis analysis was used to detect plasmid profiles. Plate transconjugation and broth transconjugation were used to examine the conjugative resistant plasmids and the agar plate dilution method was used to analyze the minimum inhibitory concentration of 15 antimicrobial agents.

用改良Kado-Liu法提取细菌质粒，琼脂糖凝胶电泳检测质粒，用平板稀释法测定药物最低抑菌浓度，用试管内肉汤接合法和平板表面接合法进行接合转移试验。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！