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The genes gB,gC and gD amplified from infectious laryngotracheitis virus Chinese Wanggang strain were cloned into the Eco RⅠ site of the eukaryotic expression vector pCAGGS,re-spectively. The positive recombinant plasmids were screened by transforming into E.coli DH5 α cells,which were named pCAGG-gB,pCAGG-gC and pCAGG-gD respectively after identification by restriction endonuclease analysis and sequencing analysis.

将传染性喉气管炎病毒王岗株的gB、gC、gD基因分别克隆到真核表达载体pCAGGS的 Eco RⅠ位点，经过酶切、测序分析，筛选鉴定出含有gB、gC、gD基因的重组质粒，分别命名为pCAGG-gB、pCAGG-gC、pCAGG-gD。

So it is assumed that the E-box might be associated with the enhancement of transcription activity.Here, the LTRs of WH17 ,DLA-25,DLA-118 and LTR with single mutation in the E-box motif were separately cloned into pCAT-Basic vector resulting in a series of recombinant plasmids containing CAT reporter under the control of different versions of LTRs.

通过对EIAV分离株WH17的LTR与L株、DA和DLA株的LTR序列进行比较，发现其在在U3-R结合处多一个E-box基序，推测该基序的变化可能会起到促进转录作用，为此将EIAV强毒株(DLA-25)、驴白细胞弱毒株(DLA-118)、EIAV分离株WH17以及U3-R结合处的E-box基序点突变的LTR分别克隆到pCAT3-basic质粒中的报告基因氯霉素乙酰转移酶的上游，获得一系列受不同LTR控制的CAT表达质粒。

Methods:The luciferase reporter plasmids of MDM2 P2 promoter containing different SNP309 were contructed,and cotransfected into CV-1 cells with AML1-ETO,AML1 and SP1 expression plasmid.The transactivities of luciferase were assayed by luminometer to determine the impact of AML1,AML1-ETO,and SP1 on MDM2 P2 promoter.

研究方法：构建含不同SNP309位点的MDM2 P2启动子的报告质粒，与AML1-ETO、AML1以及SP1的表达质粒单独或共转染CV-1细胞，测定荧光素酶的活性，分析AML1-ETO、AML1以及SP1对MDM2 P2启动子的影响。

Finally,alkaline lysis and anion exchange chromatography were used to release and purify plasmids.

研究了包括菌体生长和质粒含量等的关系。

Sequence of the MDK promoter/enhancer with Runt domainbinding sites was amplified from the human genome DNA anddirectionally cloned into pGL3-Basic Luciferase and pGL3-PromoterLuciferase reporter vectors to construct the MKP_- pGL3-Basic andMKP _-pGL3-Promoter expression plasmids.

研究方法：1。应用实时定量逆转录PCRreal-time quantitiveRT-PCR(RQ-RT-PCR，对181例急性白血病患者，其中初诊及复发患者158例、完全缓解患者23例，31例正常人骨髓标本进行了MK转录本的检测。2。

Coli XL-1 strain, purposed cassette recombination plasmids pNB0098-K and pNB0097-K were constructed, in which the purposed gene was linked with plasmid pUC18, and the marker gene kanamycin was inserted in the purposed gene. The linear cassette DNA fragment was mixed in the medium of cultured competent cells of meningococcus BT878. The function of the purposed gene was inhibited by the homologous replace of casstte gene.

将线性的重组质粒片段置于培养基中，与感受态的脑膜炎双球菌混合培养，重组质粒中的同源性片段被脑膜炎双球菌细胞识别和摄取，整合到染色体上，目的基因被带有卡那霉素基因插入的同源性重组片段代替，导致失活，在选择性培养基上筛选出基因被敲除的突变株。

Compared to MSM,method of plasmid elimination,it was proved that the genes encoding for degradation of phenanthrene locate on the larger one of the two plasmids.

与无机盐培养基相比，LB和牛肉膏蛋白胨培养基能促进此菌对菲的降解，尤其能促进对中间产物的进一步转化。

Hexon protein gene of egg drop syndrome virus, named strain E05 which was isolated from breeding muscovy duck with irregular egg dropping was amplified by PCR and cloned into pMD18-T vector. The recombinant plasmids were identified by digested restriction endonuclease and PCR. The result showed that the nucleotide length of hexon protein gene encoding 910 amino acids was 2733 bp.

运用PCR技术从表现产蛋异常的种番鸭分离鉴定的减蛋综合征病毒 E05株中分段扩增六邻体蛋白基因，分别与克隆载体pMD18-T连接后，转化感受态细胞，对经PCR鉴定和酶切鉴定均为阳性的克隆进行测序，结果表明，E05株六邻体蛋白基因长度为2733 bp，共编码910个氨基酸。

In recent years, more and more experiments indicated that RNA polymerase Ⅲ transcription might not occur in the nucleoplasm but in the nucleolus. Nevertheless, there is no direct experimental proof. In this dissertation, the plasmids bearing pol Ⅲ recognized promoters were transfected into the CHO cells by electroporation.

由于RNA聚合酶Ⅲ转录位点的研究存在特殊性，而传统的研究方法又存在一定的局限性，因此近几年人们对RNA聚合酶Ⅲ的转录发生在核质中这一结论提出了质疑，但至今尚缺乏直接的实验证据。

To further address how OSCK1 affect the pollen germination process, plasmids which can overproduce GFP-fused OSCK1 proteins were bombarded into the germinating lily pollen.

为了进一步了解OSCK1如何影响花粉管萌发，利用基因枪将融合GFP之OSCK1质体送入正在萌发的百合花粉管中，进行暂时性基因表现分析。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！