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Three RNAi target sites (named as Abil1, Abil2, Abil3) targeting the E3B1 (NCBI: NM-024397), an identified target sites and positive control GAPDH-A were selected. The pGenesil-1 eukaryotic expression vectors with the neoR mark and GFP green fluorescent mark were selected to construct E3B1 RNAi plasmid. The effect of RNAi targeting various sites on E3B1 genes expression was evaluated by testing the transfection efficiency with fluorescence microscope and western blot. The most effective siRNA in inhibiting E3B1 genes were screened. The most effective siRNA in inhibiting E3B1 genes screened and used to transfect the neuronal cells. After that, the inhibitor of axon growth was added and the axon growth was observed. Result: The siRNA expressing plasmids targeting the E3B1 were constructed successfully.

选择针对E3B1(NCBI： NM-024397)RNAi的3个靶位点Abil1、Abil2、Abil3和1个不针对任何mRNA的RNAi靶位点以及甘油醛－3-磷酸脱氢酶，用带有neoR选择标志和GFP绿色荧光标志的真核表达载体pGenesil-1构建E3B1 RNAi质粒，分别转染培养的神经元，在荧光显微镜下观测转染率，经G418筛选得到单一的转染细胞，并用Western blot法检测各转染组神经元E3B1蛋白的表达情况，选出具有最佳抑制效应的siRNA转染神经元，加入轴突生长抑制物，观测轴突生长情况。

We describe the specific silencing of reporter gene lacZ in FHM cells (muscle cells of fathead minnow, a fish cell line) by either expressing small hairpin RNAs from plasmids or transfecting small interfering RNAs transcribed in itro.

在FHM细胞内通过转染质粒表达小发夹RNA或者直接转染小干扰RNA能特异沉默报告基因LacZ的表达，且siRNAs 抑制效果更加明显；这两种类型的小双链RNA在Hela细胞内同样能抑制报告基因的表达。

BOXIT is an easy to use FileMaker Pro relational database that helps collect, store and organize information about all your antibodies, cells, oligos, plasmids/hosts and other biological samples.

是一个易于使用的关系型数据库系统，用来收集、储存、组织、管理抗体、细胞等生物样品的信息。演示版可处理15个数据。

The results showed that the both two plasmids could stablely maintained in 7653R under both free-living and symbiotic conditions and the stability was up to 100%.

结果表明：在自生与共生条件下，供试标记质粒在华癸中生根瘤菌中均能稳定存在，且稳定性达到100％。

To evaluate the safety of the DNA vaccine pVAX1/F1-V against plague, the purity of plasmid DNA pVAX1/F1-V was detected by SDS-PAGE and gelose electrophoresis method; pasmid DNA was detected by PCR in tissues of BALB/c mice immunized with the plasmids intramuscular injection.

为了研究鼠疫DNA疫苗pVAX1/F1-V质粒的安全性，试验运用SDS-PAGE电泳和琼脂糖电泳等方法检测了鼠疫DNA疫苗pVAX1/F1-V质粒的纯度，并以小鼠为动物模型进行了质粒pVAX1/F1-V的急性毒性和长期毒性试验，用PCR方法检测外源基因在小鼠组织中的分布情况，用ELISA、ELISPOT方法检测了其自身的免疫反应。

The plasmid of pMD-T-GO and pPIC3.5K were extracted from JM109 strain and then glycolate oxidase gene fragment was cloned into the expression vector (pPIC3.5K). The recombinant plasmids were transformed into E.coli TOP 10Fˊ. The recombinant clones were identified by PCR, digested with SnaB I /Not I and then sequenced.

本文首先从大肠杆菌JM109中提取质粒pMD-T-GO和pPIC3.5K，将菠菜乙醇酸氧化酶基因c DNA片段克隆至表达载体pPIC3.5K，转化进E.coli TOP 10Fˊ，利用设计的引物进行PCR扩增、SnaB I和Not I酶切鉴定，最后进行序列分析。

Objective To amplify the hexokinase gene of Plasmodium falciparum isolate FCC1/HN, compare the sequence with that of other P. falciparum isolates and human, and construct its prokaryotic and eukaryotic expression plasmids.

目的 扩增恶性疟原虫FCC1／HN株的己糖激酶编码基因，构建其原核和真核表达重组质粒，测定其序列，比较它及它推导的蛋白质与恶性疟原虫其他株和人之间的差异。

The plasmids of HA 12-1-12 were stable in different host plant, but HA 7-2-2 was much less stable.

血清型鉴定的结果证明它们确实来源于HA 7-2-2，但质粒图谱却不同。

Objective To explore the effect of cytokine encoding plasmids on DNA immunization in mice.

目的 探讨细胞因子表达质粒对小鼠DNA免疫的促进和调节作用。

They harbor indigenously circular as well as linear plasmids, a useful source for developing genetic tool for studying of their unique physiology and metabolism.

在许多稀有放线菌发现了环型与线型质粒，由质粒发展起来的遗传操作系统可以为研究稀有放线菌的生理及代谢提供强有力的工具。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！