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plasmids相关的网络例句

查询词典 plasmids

与 plasmids 相关的网络例句 [注:此内容来源于网络,仅供参考]

METHODS: Fulllength or fractions of VP22 were fused to C terminal of HBV core protein, and cloned into pcDNA3.1 vector, yielding eukaryotic expression plasmids of DN mutant.

将VP22全长及其不同区段融合于HBV核心蛋白的C端,克隆入pcDNA3.1构成DN突变体真核表达质粒。

METHODS: Fulllength or fractions of VP22 were fused to C terminal of HBV core protein, and cloned into pcDNA3.1 vector, yielding eukaryotic expression plasmids of DN mutant.

目的:了解VP22融合型显性负性突变体对抑制乙肝病毒复制的作用。

Construction of biologically functional bacterial plasmids in vitro . Proc. Natl. Acad. Sci. USA 70, 3240 – 3244 (1973).^ Patricia S. Thomas, Hybridization of Denatured RNA and Small DNA Fragments Transferred to Nitrocellulose PNAS 1980; 77: 5201-5205 PNAS Keith Roberts, Martin Raff, Bruce Alberts, Peter Walter, Julian Lewis and Alexander Johnson, Molecular Biology of the Cell

一个很典型的例子就是DNA分子大小的测量:在凝胶电泳出现之前,DNA分子大小是用蔗糖密度梯度沉降法来测量的,这一方法费时费力而且花费昂贵;而在梯度沉降法出现之前,使用的是黏度法。

In order to express the recombinant peptide of both gp52 and pp150 Oterminal peptides from human cytomegalovirus in the Pichia pastoris, the recombinant plasmids were transformed into Pichia pastor is by electroporation after linearised respectively by Sac I or Bg1II, the transformants were screened in MD plates without histidine.

为了在巴斯德毕赤酵母中表达人巨细胞病毒gp52C末端和pp150C末端串联片段的嵌合肽,将构建好的hCMVp-pPIC9K重组质粒分别经Sac Ⅰ和Bg1 Ⅱ酶切,电打孔法导入毕赤酵母GS115后,在缺组氨酸的MD板上筛选出转化子。

The BALB/c mice were immunized i.m. with the plasmids for 3 times in 3 weeks intervals.

用重组真核表达质粒pcDNA3 1 /HRP Ⅱ经肌肉免疫小鼠 3次,每次间隔 3wk。

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