查询词典 plasmids

In genetic engineering, multiple identical copies of a gene are produced in cloning vectors, such as plasmids and phages.

在遗传工程上，相同的多拷贝基因是由质粒或噬菌体这样的克隆载体产生的。

New phages can develop by acquiring restriction enzymes from plasmids.

通过从质粒获得的限制性酶，新的噬菌体能够生长。

Cloning of DNA is made possible by the ability of bacterial plasmids and phages to reproduce after additional sequences of DNA have been incorporated into their genomes.

将一段额外的DNA序列整合到细胞质粒和噬菌体的基因组上进行繁殖，使DNA克隆已成为可能。

It was demonstrated that the dependence or independence of recA gene is not related to the direction of replication of the plasmids or phages in the free state.

实验结果说明，依赖与否和它们在游离状态中复制方向无关，从而否定了recA基因的作用是把质粒或噬菌体的游离状态的单向复制发动机构改变为整合状态的双向复制发动机构这一假设。

All the recombined suicide plasmids were successfully obtained and sequenced, and 24 unique sequences were identified. Bioinformatics analysis showed that there were 51 ORFs in the 24 operons.

对插入的随机片段进行测序后进行生物信息学分析，共证实有24个不同的基因片段，在TIGR4全基因组里面，这24个基因片段所处的操纵元结构中，总共包括51个开放阅读框，其中多数为含有多个ORFs的操纵元结构。

The capacity of the primary library was 5.0×105 PFU/mL and the capacity of the amplified library was 8×109 PFU/mL. The cDNA library was immunoscreened by the rabbit serum against H.qinghaiensis larval tick, the positive pluques were subcloned, and their plasmids extracted and then transformed into Escherichia coli JM109. Eventually, 11 genes were obtained and designated HqL1,HqL2,HqL3,HqL4,HqL5,HqL6,HqL7,HqL8,HqL9,HqL10 and HqL11,respectively.

采用常规方法构建了青海血蜱幼蜱cDNA表达文库，初级文库的库容量为5.0×105PFU/mL，扩增后的库容量为8×109PFU/mL；用兔抗青海血蜱幼蜱阳性血清对该文库进行了免疫学筛选，对阳性噬菌体进行了亚克隆，提取质粒后转化宿主菌JM109，最终共得到幼蜱基因11个，分别命名为HqL1、HqL2、HqL3、HqL4、HqL5、HqL6、HqL7、HqL8、HqL9、HqL10、HqL11。

All index proved that the P5776 cDNA library constructed in this research had high quanlity and could be used for further ESTs sequencing and analyzing. The amplified library of P5776 was screened on white-blue plates, and more than 5,000 white-clones were selected to culture in 96-well plates, then the high quanlity plasmids of white-clones were extracted by traditioal alkaline-lysis method using the Vitagene?96-easy plasmid extract kit. Through PCR primered by T3( the vector has T3 RNA polymerase promoter ), the target fragments of inserts were get. Then the inserts were taged by four different fluorescence-dye, and fractionated by capillary in ABI Prism? sequencer. More than 5,000 5′-ESTs had been sequenced and through rough selecting, 4,747 ESTs were remained for further research. Among the remained ESTs, more than 90% have longer lenth than 500bp, and there are less than 5% unable-read bases in every sequence.

将扩增好的P5776 cDNA文库铺布蓝白斑筛选平板，挑取其中的白斑经活化培养后，运用Vitagene 96-easy质粒DNA制备试剂盒以碱裂解法共提取出58 板(96孔板)，即5，000 余个重组子克隆的高质量质粒DNA；提取好的质粒以T3(连接载体上具有该引物RNA聚合酶启动子)为引物经PCR(Polymerase Chain Reaction，聚合酶链式反应)扩增出重组子插入片段，标记上荧光染料后在ABI Prism 3100 测序仪上经毛细管电泳完成了5，000 余条5′端EST 序列的测定工作，初步筛选出4，747 条质量较好的EST序列，经筛选得到的EST中90%以上具有大于500bp 的可读序列，每一EST序列中不可读碱基数小于5%。

Results Identification by enzyme restrictions and PCR amplifications, as well as DNA sequencing confirmed that the plasmids were successfully constructed.

结果 限制性酶切、PCR扩增和DNA测序证明质粒构建成功。

Genes located on chromosomes, plasmids, or transposons encoding specific resistance to a variety of metal ions.

对重金属产生抗性的基因位于微生物的染色体、质粒和转座子上。

AIM摘要: To construct the recombinant plasmids expressing fulllength HCV core protein gene and 3 different deletion mutated hepatitis core protein genes and to express them in E.coli.

目的摘要：构建丙型肝炎病毒全长及3种不同缺失突变的核心蛋白基因的原核表达载体，并在大肠杆菌中表达。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！