查询词典 plasmids

The two reconstructed plasmids were packed to infective virus particles in incasing cells PT67 with a tite of about 5. 8×10〓-3. 4×10〓cfu/ml.

2两种重组表达质粒在PT67细胞中包装成具有感染能力的病毒颗粒，产生病毒滴度在5.8×10〓-3.4×10〓cfu／ml之间。

The two reconstructed plasmids were packed to infective virus particles in incasing cells PT67 with a tite of about 5.8xl05-3.4xl06cfu/ml.

转基因治疗有效组小鼠的一般状况如精神、活动等表现较好，体重减轻较少，外周血白细胞低下的时间更短。

It could be useful for the further study of NS1 gene function. Method The NS1 gene of H5N1 subtype AIV was amplified by RT_PCR and cloned to pGEM_T_easy vector. The NS1 gene expressing plasmids was constructed by inserting the target gene fragments into pGEX_4T_1 vectors. The expression proteins were indued by IPTG and analysed by Western blot.

方法采用RT_PCR方法对H5N1亚型AIVNS1基因进行扩增，将PCR产物克隆于pGEM_T_easy载体，将该基因插入pGEX_4T_1中构建NS1基因原核表达载体，转化BL21大肠杆菌后，在IPTG诱导下表达NS1蛋白，Westernblot鉴定表达NS1蛋白。

To test this hypothesis, we performed experiments using plasmids containing a luciferase reporter with amber, ochre and opal nonsense mutations within the luxB gene in Escherichia coli.

为验证此假设，我们用几个含有大肠杆菌荧光素酶报告基因的质粒进行了实验，这些质粒中的luxB基因分别包含有琥珀色、赭色和乳白色的无义突变。

The bacteria containing the recombinant plasmids were induced with IPTG and samples of bacteria culture fluid were collected at different time respectively.

重组阳性菌用IPTG诱导表达，收集不同培养时间的菌液进行SDS-PAGE和Western blotting。

Methods Fourteen mutated vpr fragments were selected from patients with HIV. Both eukaryotic expression vector pcDNA3.1 and PCR products were purified, double-cut by Hind Ⅲ and BamH Ⅰ, and the cut products were legated and transformed into competent cells JM109. The 14 reconstructed plasmids were transfected into Hela cells. Cells with pcDNA vpr-wt, pcDNA vpr-Fs and pcDNA3.1 blank cells, and without pcDNA3.1 cell were established.

以14个带有HIV-1vpr基因片段的PcD-NA3.1真核表达载体构建重组质粒，将其转染Hela细胞，并设立保守株vpr基因转染细胞、突变株vpr-FS基因转染细胞、空载体转染细胞和未转染细胞作为对照，经逆转录多聚酶链式反应检测目的基因转染成功后，Pi染色，用流式细胞仪检测被转染细胞的细胞周期分布和细胞凋亡率。

Methods 14 mutanted vpr fragments selected from Chines patients with HIV. Both eukaryotic expression vector pcDNA3.1 and PCR products purified , double-cut by HindⅢ and BamH and the cut products legated and were into competent cells JM109. The 14 reconstructed plasmids electronically transfected into hela cells, and established cells with pcDNA vprwt 、pcDNA vpr-Fs and pcDNA3.1 blank cells, and without pcDNA3.1 cell.

将14个带有HIV-1 vpr基因片段的pcDNA3.1真核表达载体构建重组质粒，将其转染Hela细胞，并设立保守株vp r基因转染细胞、突变株 vpr-FS基因转染细胞、空载体转染细胞和未转染细胞作为对照，经RT-PCR检测目的基因转染成功后，经Pi染色用流式细胞仪检测被转染细胞的细胞周期分布和细胞凋亡率。

This paper reported the construction of the recombinant expression plasmids of salmon calcitonin gene for the first time.

报道了鲑鱼降钙素基因植物表达载体的构建及其向烟草的遗传转化。

RESULTS: All the four recombinant eucaryotic plasmids were constructed successfully, and HBV core protein expression was confirmed by internal reference for transfection.

结果： 4份重组真核表达质粒均构建成功，转染至HepG2后通过内参照EGFP可鉴定出均有HBV-C蛋白表达。

OBJECTIVE:To study the effects of eukaryon expressed adiponectin on the glycogen synthesis and glucose oxidation in skeletal muscle cell strain C2C12 myotubes by transfecting plasmids carrying mouse adiponectin.

目的：通过转染带小鼠脂联素基因的质粒，观察真核表达的脂联素对骨骼肌细胞株C2C12肌小管葡萄糖氧化和糖原合成的影响。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！