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Methods: Genes of MCP and DAF were connected by 10aa coding sequence of enriching Gly and Ser. Then insert into pcDAN3.1 vector, constituting pcDNA-MD3 expression plasmid of hMCP-DAF fusion gene. The genes were transformed by liposome into the pig endothelial cell; human serum handles masculine cloning, identify the function that restrain breaking of person alexin.

将MCP、DAF两个基因以富含Gly和Ser的10aa编码序列作铰链串接，并接入表达载体pcDNA 3.1中，构成含hMCP-DAF的真核表达质粒(pcDNA-MD3)；以脂质体转染猪内皮细胞，人血清处理阳性克隆细胞，鉴定抑制人补体溶破的功能；应用显微注射将线性基因导入猪受精卵；PCR检测导入基因整合率。

A colorectal cancer cell line and a normal amnion cell line were transfected by this plasmid.

这些结果表明hTERT－CD／SFC系统在体内外均具有显著的抑瘤效果，且具有高度的肿瘤特异性。

The BDDcFⅧ gene was ligated behind PUB and 20H1 promotors to create lentiviral vectors pTK161 and pTK162. Meantime lentiviral vectors pTK161' and pTK162' were produced by cloning a green fluorescent protein into pTK151 and pTK152, which was driven by PUB and 2OH1 promotors respectively. Vector supernatant were prepared by using transfer calcium phosphate mediated-cotransfection of 293T cells. The virus vector,△NRF packaging-plasmid, and VSV-G envelope-plasmid was assayed by titers and cFⅧ activity in cell culture supernatant after infection into 293T cells.

用构建携带cFⅧ基因的慢病毒载体pTK161和pTK162（含20HI启动子），同时构建含绿色荧光蛋白的慢病毒载体pTK161'和pTK162'（含2OH1启动子）的方法，分别与包装质粒△NRF、包膜蛋白质粒VSV-G共转染293T包装细胞，将包装好的病毒颗粒再感染293T细胞，检测病毒滴度和培养细胞上清中cFⅧ活性。

objective to explore plasmid dna extraction and purification methods as well as measure plasmid dna.

目的 探讨提纯质粒dna的方法并进行检测。

Co-transformation of plasmid pGg-gfp and plasmid pCc1001 which harbors the complementary gene trp1 was conducted by the PEG-mediated protoplast transformation of the oidia of LT2, a tryptophan auxotrophic strain of Coprinus cinereus.

采用PEG介导法把表达质粒pGg-gfp与辅助质粒pCc1001（含有trp1基因）共转化进色氨酸营养缺陷型的灰盖鬼伞粉孢子的原生质体中。

After the animal modle done successfully, all animals were randomly divided into two groups. group l: Microbubble contrast agent containing plasmid was injected into nude mice via the tail vein; group 2: the mixture of microbubbles with plasmid was injected as the group 1, and eyeballs were exposed to ultrasound with intensity of 0.5W/cm^2 immediately, and the time were all 60s that working time control at 20%.

将12只BAlB/C裸小鼠双眼玻璃体腔接种HXO-Rb44细胞，造模成功后，将动物随机分为2组，第1组于尾静脉注入含质粒的微泡造影剂；第2组尾静脉注射质粒与微泡的混合液，并立即以0.5W/平方公分〔1〕的超声波辐照小鼠眼球60s，工作时间控制为20%。

The toxin-antitoxin system was originally found on the low-copy plasmid to maintenance the plasmid stability.

毒素-抗毒素系统最早发现于一些低拷贝的质粒，用来维持低拷贝质粒在菌群中的稳定存在。

Using plasmid pUT 649 as a model plasmid,the extraction of DNA by reversed micelles system of methyltrioctylammoniumchloride/2-ethylhexanol/isooctane was studied.

采用新的TOMAC/2-乙基己醇/异辛烷反胶团体系，对该反胶团萃取质粒pUT649进行了研究。

METHODS: Human cbl gene tagged with flag was amplified from pEFHAcbl plasmid by PCR. The product was cAIM: To construct the eukaryotic expressing vector of human cbl and test its expression in African green monkey kidney cell line COS7. METHODS: Human cbl gene tagged with flag was amplified from pEFHAcbl plasmid by PCR. The product was cloned into pGEMT easy, sequenced and then subcloned into eukaryotic expressing vector pcDNA3.1.

以含有人全长cbl cDNA的质粒pEFHAcbl 为模板，采用PCR方法扩增cbl基因，并在其N末端带上含24 bp的flag标签，克隆到pGEMT easy载体并测序，再亚克隆至真核表达载体pcDNA31，酶切鉴定正确后采用脂质体法瞬时转染COS7细胞，Western blot检测flagcbl在细胞中的表达。

Xanthus DK1622 by PCR, and inserted the fragment into a site upstream of lacZ, resulting in the recombinant plasmid pZCY13. The plasmid pZCY13 was transformed by electroporation to DK1622, producing a mutant ZC16-18 ?

从黄色粘球菌DK1622基因组上PCR扩增MXAN1334基因内部部分片段，插入载体pZCY11上lacZ基因的上游，构建重组质粒pZCY13，将其转入DK622菌株，获得MXAN1334基因插入失活突变株ZC16-18。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！