查询词典 plasmid
- 与 plasmid 相关的网络例句 [注：此内容来源于网络，仅供参考]
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The apxⅢA gene of Actinobacillus pleuropneumonie was amplified by PCR. The amplified DNA fragment 3 466bp was cloned into pMD18-T. After R.E. analysis and sequencing, the apxⅢA gene in pMD18-T was ligated into pBluescripⅡSK, the recombinant expression plasmid pET-28b/apxⅢA was constructed and analysed with R.E., the protein of apxⅢA gene expressed in E.coli BL21 was detected by Western blotting.
因胸膜肺炎放线杆菌的致病性主要是由毒素决定的，故参照猪胸膜肺炎放线杆血清2型菌株的序列(GenBankL1 2 1 4 5)设计了一对特异性引物，用PCR的方法扩增apxⅢA基因并得到了长 3 466bp的片段，然后将其克隆到pMD 1 8T中，经酶切鉴定和序列分析表明克隆是成功的；再将apxⅢA插入到原核表达载体pET 2 8b后，转化BL2 1 (DE3)，在IPTG诱导下获得高效表达，经Westernblotting检测证实表达产物有活性。
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Objective To study the mechanism of apoptosis in Human Gastric carcinoma cells BGC-823 Induced with recombinant plasmid comprised Hemagglutinin-neuraminidase Gene from Newcastle Disease Virus.
目的本实验研究旨在探讨含新城疫病毒HN基因的重组质粒对人胃癌细胞BGC-823凋亡的联合作用机制。
- 推荐网络例句
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She gently rebuff ed him, but agreed that they could be friends
她婉言拒绝了，但同意作为朋友相处。
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If in the penal farm, you were sure to be criticized.
要是在劳改农场，你等着挨绳子吧！
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Several theories about reigniting and extinguishing of the arc have been refered.
本文综合考虑了几种电弧重燃和熄灭理论。