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AIM: To construct the eukaryotic expressing vector of human cbl and test its expression in African green monkey kidney cell line COS 7. METHODS: Human cbl gene tagged with flag was amplified from pEFHAcbl plasmid by PCR. The product was cloned into pGEM T easy, sequenced and then subcloned into eukaryotic expressing vector pcDNA3.1.

以含有人全长cbl cDNA的质粒pEFHAcbl 为模板，采用PCR方法扩增cbl基因，并在其N 末端带上含24 bp的flag标签，克隆到pGEM T easy载体并测序，再亚克隆至真核表达载体pcDNA3 1，酶切鉴定正确后采用脂质体法瞬时转染COS 7细胞，Western blot检测flag cbl在细胞中的表达。

To determined the role of this gene in gluconeogenesis and in pathogenesis of Xcc, mutants of pgi gene were constructed by homology plasmid integration method.

六磷酸葡萄糖异构酶是糖异生途径中另一个关键酶，在野油菜黄单胞菌中编码该酶的基因是pgi基因(Xcc 8004基因组中基因编号为XC2379)。

Abstract—A yeast plasmid was constructed to contain a hybrid GAL–CYC promoter, the NPTII neomycin phosphotransferase gene, and the FRT sequence between them.

摘要，酵母质粒构建了含有混合放射公益少年团启动，新霉素的NPTII 磷酸转移酶基因，并首次登记税它们之间的序列。

The introduced kan gene in EBV-BAC genome was eliminated specifically by the recombinase FLP when transformed by plasmid pCP20, leaving an FRT scar of 69 bp.

这种经改进并程序化的方法，也适应于引入其它突变或在其它BAC-疱疹病毒基因组中引入突变。

It was also found base transversion had a extremely high ocurrence frequency in the single base substitution mutants when the plasmid was replicated in FL-FEN-1〓 cell or MNNG pretreated FL-FEN-1〓 cell, while in all mutants with multiple base substitution there were at least one transversion mutation taken place.

分析还发现，在FL-FEN-1〓细胞自发突变和非定标突变含有单碱基替换突变的突变子中，碱基颠换的发生率极高，而在FL-FEN-1〓细胞自发突变和非定标突变的多碱基替换突变子中，每个突变子均至少含有一个碱基颠换突变。

In contrast, the improv ement of HCV core-specific CTL activity with an IL-2-producing construct coimmuniza tion was not obvious, which maybe resulted from the low-level expression of IL -2 vector-pDOR. CONCLUSION DNA vaccine of HCV combined with IL-12 expr essive plasmid can get better therapeutic effects, and it might represent an approach to the gene therapy against HCV infection.

佐以不同的细胞因子，可增强基因疫苗诱生的免疫应答强度，可能使机体对基因疫苗的免疫应答朝着有利于优先诱导CTL免疫应答，清除病原体的方向发展。

A eukaryotic expression plasmid of Fas／APO－1 gene was constructed successfully.

本实验成功地构建了Fas／APO－1基因的真核表达质粒。

Transforming DNA uptake gene orthologs do not mediate spontaneous plasmid transformation in Escherichia coli. J Bacteriol.

近年来研究表明肠道中大量存在的大肠杆菌具备自然转化能力，因此转基因食品中的基因有可能通过大肠杆菌进入人体肠道细胞。

Xanthus. The plasmid vector pZCY11 made it more convenient for the study on functions and the expressions of target gene in M. xanthus.

构建的质粒载体pZCY11不仅能够对目的基因进行功能的分析，而且能够同时通过报告基因分析基因的表达情况，可简化粘球菌中基因功能及表达情况的研究。

89 Strains of shigella isolated from 3 different areas( Chengtu clty,Yuxi district,Da Xingang)in 1990 were analysed by plasmid profiles and antibiotic resistance patterns.

本文对成都市、玉溪地区、大新港三地1990年分离的89株痢疾杆菌进行了质粒图谱分析及耐药谱分析。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！