查询词典 plasmid

Viciae hurL gene formed nodules in wild-type amounts, indicating that hurL gene is required for nodulation. Although the function of hurL gene in inducing nodulation remains unknown, the results of this work revealed that in addition to the nod and exo genes located on Sym plasmid, the R. leguminosarum chromosomal hurL gene is also involved in controlling its capacity in inducing nodulation on host plant.

虽然目前对hurL基因影响豌豆根瘤菌诱导植物结瘤能力的机制并不清楚，但本文的研究结果首次证明：除位于共生质粒上的结瘤基因和胞外多糖基因外，豌豆根瘤菌在宿主植物上的结瘤还受到染色体基因hurL的控制。

Viciae hurL gene formed nodules in wild-type amounts, indicating that hurL gene is required for nodulation. Although the function of hurL gene in inducing nodulation remains unknown, the results of this work revealed that in addition to the nod and exo genes located on Sym plasmid, the R.

虽然目前对hurL基因影响豌豆根瘤菌诱导植物结瘤能力的机制并不清楚，但本文的研(来源：Acf6fBf6C论文网www.abclunwen.com)究结果首次证明：除位于共生质粒上的结瘤基因和胞外多糖基因外，豌豆根瘤菌在宿主植物上的结瘤还受到染色体基因hurL的控制。

P210bcr/abl plays a central role in the pathophysiology of CML. The purpose of this study was to construct a cell line model that bcr/abl expression can be regulated by Tetoff inducingexpressionsystem. The fulllength b3a2 bcr/abl cDNA was subcloned into the pTRE2hyg expression vector to construct the pT2P210 plasmid.

CML是目前致病分子信号基础研究最为清楚的肿瘤之一，1960年Nowell和Hungerford在CML患者的白血病细胞中发现Ph染色体［1］，1973年Rowley等［2］进一步应用染色体分带技术证明，Ph染色体是由9q34的abl基因和22q11 的 bcr 基因相互易位形成的 bcr/abl 融合基因。

Precomputed comparisons can be selected from a database covering all the completed bacterial chromosome and plasmid sequences in the Genome Reviews database.

预计算的比对能够从覆盖所有完整的细菌染色体和质粒序列的一个数据库中挑选。

In order to study the expression, subcellular distribution and the function of NS2 protein, the coding region of NS2 was amplified from the hindgut tissue of cockroaches infected with PfDNV by RT-PCR and then the recombinant prokaryotic expression vector pET28a-NS2 was constructed. The recombinant plasmid was transformed into E. coli BL21 (DE3) to express the 6×His fusion protein in the bacteria.

为了对该基因进行深入的功能研究，从感染了黑胸大蠊浓核病毒的蟑螂的后肠组织中通过RT-PCR得到ns2基因编码序列，将其构建于原核表达载体pET-28a，转化大肠杆菌BL21(DE3)获得融合表达产物。

In this paper the conception of horizontal gene transfer was introduced, and main mode of HGT was also enumerated as follows: HGT by medium such as plasmid and virus etc. and the HGT without any medium.

摘 要：简要介绍了水平基因转移（horizontal gene transfer， HGT）的基本概念以及进行转移的主要方式：由质粒或病毒等介导的水平基因转移和基因的&直接&水平转移。

In this paper the conception of horizontal gene transfer was introduced,and main mode of HGT was also enumerated as follows:HGT by medium such as plasmid and virus etc.and the HGT without any medium.

简要介绍了水平基因转移（horizontal gene transfer，HGT）的基本概念以及进行转移的主要方式：由质粒或病毒等介导的水平基因转移和基因的&直接&水平转移。

The expression of PCDNA〓-EGFP plasmid was monitered withfluorescence microscope,and the human thyroid undifferentiated carconoma in situmodel and haematogenous metastasis model were established in nude mice withPCDNA〓-EGFP positive cells.These model were used in discussing tumourmetastasis mechanism and early diagnosis of secondery tumour.

荧光显微镜观察〓-EGFP质粒表达情况，并用转染了PCDNA〓-EGFP质粒的细胞建立人甲状腺裸鼠原位及血源移植模型，探讨其转移机制及转移灶的早期发现。

The results of transient transfection showed that the recombinant plasmid could be lowly expressed in the murine macrophage cell line RAW264.7 and murine melanoma cell line B16, both of which can endogenously express Tim-3, but not in the African green monkey kidney cell line COS-7 in which no endogenous Tim-3 could be detected.

结果 pGL3-Tim-3P2经PCR、双酶切及DNA测序分析鉴定正确无误；pGL3-Tim-3P2转染RAW264.7和B16细胞（两种细胞均可表达内源性Tim-3）24 h和48h，双荧光素酶活性检测显示其启动子相对活性约为pGL3-Basic空载体的2倍；而pGL3-Tim-3P2转染不表达内源性Tim-3的COS-7细胞后检测不到荧光素酶活性。

The African green monkey kidney cell line, Vero, was used as the host for plasmid DNA and MNNG (N-methyl-N′-nitro-N-nitrosoguanidine) was chosen as the DNA-damaging agent.

实验采用非洲绿猴肾细胞株Vero和MNNG(N-methyl-N′-nitro-N-nitrosoguanidine)分别作为质粒DNA的宿主和损伤剂。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！