查询词典 plasmid

All index proved that the P5776 cDNA library constructed in this research had high quanlity and could be used for further ESTs sequencing and analyzing. The amplified library of P5776 was screened on white-blue plates, and more than 5,000 white-clones were selected to culture in 96-well plates, then the high quanlity plasmids of white-clones were extracted by traditioal alkaline-lysis method using the Vitagene?96-easy plasmid extract kit. Through PCR primered by T3( the vector has T3 RNA polymerase promoter ), the target fragments of inserts were get. Then the inserts were taged by four different fluorescence-dye, and fractionated by capillary in ABI Prism? sequencer. More than 5,000 5′-ESTs had been sequenced and through rough selecting, 4,747 ESTs were remained for further research. Among the remained ESTs, more than 90% have longer lenth than 500bp, and there are less than 5% unable-read bases in every sequence.

将扩增好的P5776 cDNA文库铺布蓝白斑筛选平板，挑取其中的白斑经活化培养后，运用Vitagene 96-easy质粒DNA制备试剂盒以碱裂解法共提取出58 板(96孔板)，即5，000 余个重组子克隆的高质量质粒DNA；提取好的质粒以T3(连接载体上具有该引物RNA聚合酶启动子)为引物经PCR(Polymerase Chain Reaction，聚合酶链式反应)扩增出重组子插入片段，标记上荧光染料后在ABI Prism 3100 测序仪上经毛细管电泳完成了5，000 余条5′端EST 序列的测定工作，初步筛选出4，747 条质量较好的EST序列，经筛选得到的EST中90%以上具有大于500bp 的可读序列，每一EST序列中不可读碱基数小于5%。

Gene therapy ; DNA vaccine ; plasmid ; ion exchange chromatography

基因治疗； DNA疫苗；质粒；离子交换色谱

The recombinant plasmid pBI121-VP6 was transformed into Agrobacterium tumefaciens strainLBA4404 by freeze-thawing method and the introduced into the sterile tube culture shoot of tobacco NC89 and baoding alfalfa by leaf disc infection method.

以冻融法将pBI121-VP6重组质粒导入根癌衣杆菌LBA4404，用叶盘法转化于NC89烟草和保定紫花苜蓿的无菌试管苗。

Devise a pair of primer to put out the section of pgE which code the dense antigen episode, clone into pET-32a, name the masculine plasmid with pET-32pgE. Transform the BL21 strain with pET-32pgE, choose several single bacterium drops at random and contaminate 2X YT. vibrate at 37癈 over night. Contaminate another tube with the above culture to the final concentration of 2%, continue to culture to OD6oo0.6, add IPTG to the final concentration of Immol/L. inducing 6 hours. Analyse the expression with SDS-PAGE and Western-blot, the result is that the expression has actually happened and the product has immunoreactivity.

设计一对引物从gE全基因克隆载体pgE中调出抗原决定簇密集编码区，克隆进PET-32a表达载体，双酶切和PCR鉴定出阳性者，命名为PET-32pgE，转化BL21菌株，挑取单菌落在加有氨苄的2×YT培养基中37℃过夜长至饱和，按2％体积转种另一管，培养至OD_(600)为0.6，加IPTG至终浓度1mmol／l，诱导4～6小时，SDS-PAGE和Westernblot分析，证明确实表达并具有免疫反应性。

Used the method of touch down PCR, the ACS2 gene was cloned from DNA of Petunia hybrida. At the same time, its gene sequence DQ090003 was obtained in GenBank, the antisense repetition of ACS2 linked the plasmid pBI-121 by use of one step. In the result, the expression vector having antisense repetition gene was constucted.

利用降落PCR的方法，从矮牵牛DNA中扩增出ACS2基因片段，在GenBank中获得基因序列号为DQ090003；利用一步法将ACS2基因的反义重复与pBI-121质粒连接，构建含该基因的反义重复表达载体。

The recombinant plasmid was transformed into its host E.coli strain BLai and a protein band with MW of 27kD was expressed.

将重组质粒转化大肠杆菌BL_(21)株，经IPTG诱导，表达分子量约为27KD的融合蛋白。

And to identify by nucleotide sequencing and restriction endonuclease digestion.(2) The recombinant plasmid was transformed into Bacillus coli and to shake culture.

将pET28a-hnRNPA2/B1重组质粒转化入大肠杆菌BL21振荡培养，用IPTG诱导表达融合蛋白并且优化条件，以SDS-PAGE分析融合蛋白的存在及可溶性。

The most pathogenic isolate, VIB 645, contained two closely related hemolysin genes, which were cloned and sequenced. In this study, vhhA was cloned into an expression plasmid pET-24d.

本研究将哈维氏弧菌的溶血素基因vhhA克隆到大肠杆菌的表达载体pET-24d，VHH溶血素作为一种带六个组氨酸的融合蛋白在大肠杆菌表达菌株BL21(DE3)中得到了过量表达，重组大肠杆菌及其培养上清液在鱼血平板上有很强的溶血活性。

The complex has also been found to promote the cleavage plasmid pBR 322, in the presence of H_2O_2 and ascorbic acid.

所有的实验结果表明配合物与CT-DNA是通过非经典或部分嵌插模式相互作用的。

Results The recombinant plasmid pSNAV2.0-TK was verified byPCR and restricton endonuclease. The coat protein of AAV was detectedby SDS-PAGE method, the purity of rAAV2/HSV-TK was＞98% byHPLC method and the titre of rAAV2/HSV-TK was 1×10~(12)v.g./ml byDNA dot blot hybridization.

结果：重组质粒pSNAV2.0-TK PCR扩增的片段大小和酶切鉴定所切下的片段与预计结果一致；SDS-PAGE法检测到三条特征性的AAV外壳蛋白条带，HPLC法检测rAAV2／HSV-TK的纯度＞98％；用DNA斑点杂交方法检测rAAV2／HSV-TK的滴度为1×10~(12)v.g。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！