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The protein interaction domains was delineated at the N-terminal 50-amino-acid fragment of HCV core protein and the C-terminus of p53. Confocal analysis also revealed that these two proteins colocalize in subnuclear granules and peri-nuclear region. Transfection experiments using a p53-responsive reporter plasmid in HuH-7, Hep3B, HepG2 and H1299 demostrated that full-length HCV core protein could elicit a positive or negative effect on the p53-mediated transcriptional activation depending on the concentration of the HCV core protein.

更且利用p53蛋白C端删除55-95个胺基酸之质体与含p53蛋白结合区之报导基因和表现全长HCV核心蛋白三者之质体於H1299细胞株进行共同转染时，与全长p53蛋白比较，发现HCV核心从增强全长p53蛋白之转活化能力转为抑制p53蛋白缺乏C端55-95个胺基酸之转活化能力，因实验室已有结果证实活体外及活体内缺乏C端55个及75个胺基酸之p53蛋白无法与HCV核心蛋白进行结合(Kao， unpublished data)。

The plasmid DNA remained intact and could be stored at 4℃ for at least one month in liposomes, it was also resistent to DNase I digestion.

重组质粒DNA在脂质体的制备过程中保持了稳定性，并能抵抗DNA酶的水解破坏作用。

Based on the sequence similarities among minimal replicon modules, p141 belonged to Group III family in the RCR plasmid.

根据rep基因的相似性比较，判断p141的复制模式归属于RCR模式的Group III组，即pC194家族。

In this study, in utero transfection plasmid DNA method was set up to embryonic mouse. The GFP expression vector pCAGGS-IRES-EGFP was microinjected into the lateral cerebral ventricle of E 14.5d mouse embryos through the uterine wall. Then, the 30 V square-wave pulse was delivered across the head for 5 times and interval 50m sec by electrodes in parallel with the median raphes.

本研究旨在通过子宫内电穿孔法，建立鼠胚大脑皮层体内基因转移体系：将带有绿色荧光蛋白的表达载体pCAGGS-IRES-EGFP显微注入胚胎期14.5 d的鼠胚侧脑室中，电极沿与脑中缝平行的方向夹持鼠脑，在电压30 V，脉冲时间50 ms，脉冲数5的条件下方波电击，将外源基因转移到室管膜层细胞。

Objective To acquire enough envelope glycoproteins so as to facilitate a further study of the structure and function of envelope glycoproteins from various kinds of virus isolates. Methods Two envelope glycoprotein gene fragments were cloned from the recombinant plasmid pHXB2 containing the human immunodeficiency virus 1(HIV-1) proviral genome of HXB2 isolate.

目的 获得足够量的膜糖蛋白，以便于对不同HIV分离株膜糖蛋白的结构与功能进行进步的研究方法从人免疫缺陷病毒1(HIV-1)HXB2分离株原病毒基因组的重组质粒pHXB2中克隆了两段膜糖蛋白基因片段。

Methods Two envelope glycoprotein gene fragments were cloned from the recombinant plasmid pHXB2 containing the human immunodeficiency virus 1(HIV-1) proviral genome of HXB2 isolate.

从人免疫缺陷病毒1(HIV-1)HXB2分离株原病毒基因组的重组质粒 pHXB2中克隆了两段膜糖蛋白基因片段。

Methods Two envelope glycoprotein gene fragments were cloned from the recombinant plasmid pHXB2 containing the human immunodeficiency virus 1(HIV-1) proviral genome of HXB2 isolate.

从人溃疡免疫缺陷病毒发烧1(HIV-1)HXB2分离株原病毒发烧基因组的重组质粒pHXB2中克隆了两段膜糖蛋白基因片段。

This recombinant plasmid will be a useful tool in the studies of the inductive mechanism of λ prophage and the repair of cells damaged by UV-irradiation.

这种重组质粒将在进一步研究λ原噬菌体的诱导机理以及受紫外辐射损伤细胞的修复作用等方面成为有效的工具。

Offspring of the fertilized eggs whose pronuclei were injected of the plasmid were screened for transgene expression.

接著此载体以显微注射递送到小鼠受精卵中，其子代以聚合脢链锁反应筛选转殖基因是否嵌入其基因体。

The different of the two plasmid lied in different Promotors ,since strong paomotor may paly a important role in gene expression in Virat vector .

他们的区别在于含有不同的启动子，因为强启动子可能在病毒载体系统的基因表达上起关键的作用。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！