查询词典 plasmid

Objective To construct recombinant plasmid of Legionella pneumophila mip gene and detect its expression in NIH3T3 cells.

目的 构建嗜肺军团菌mip基因的真核表达重组质粒pcDNA3.1-mip，并研究其在细胞中的表达。

AIM:To investigate the effects of pEGFP-TGF-β1 plasmid DNA on proliferation and survival of human lens epithelial cell.

摘要目的：探讨脂质体介导的TGF-β1基因(pEGFP-TGF-β1)转染对人晶状体上皮细胞系B3(HLEC- B3)增殖、凋亡及细胞周期的影响。

Using the total RNA of merozoites of Eimeria acervulina isolated from Guangdong Pro- vince as template,a partial segment of 3-1E gene was amplified by RT-PCR. The gene fragment 682 bp inlength was cloned into pGEM-T Easy vector,and the recombinant plasmid was identified by PCR,restric-tion enzyme analysis and sequencing. The homology analysis revealed that the nucleotide homology be-tween the 3-1E gene of the E.

根据GenBank中登录的堆型艾美球虫3-1E基因序列，设计了3条引物，以广东株堆型艾美球虫裂殖子总RNA为模板，利用反转录-聚合酶链反应扩增获得了3-1E基因部分片段，将这一片段克隆至pGEM-T Easy载体中，经PCR、限制性内切酶分析和克隆片段的序列测定、比较，证实了克隆片段的可靠性。

Objective: To study the anti-tumor effect of PTEN eukaryotic expressing plasmid on human bladder cancer xenografts in nude mice.

目的：探讨PTEN真核表达质粒对人膀胱癌裸鼠移植瘤的抑制作用。

Objective To search for the best approaches to transfect plasmid pEGFP-N1 into human fibroblasts.

目的 采用3种不同的方法将质粒pEGFP-N1转染人成纤维细胞，从中寻找最佳的稳定转染的方法。

The plasmid of pcDNA3/bcl????2 was used to transfect GES????

2基因表达的人胃上皮细胞系GES？？？？

OBJECTIVE: To transfect rabbit bone marrow mesenchymal stem cells using pIRES2-EGFP-BMP-2 eukaryotic expression plasmid.

目的：使用前期已经构建成功的pIRES2-EGFP-BMP-2真核表达质粒转染兔骨髓间充质干细胞。

METHODS: Lipofectamine method: The BMMSCs were obtained from the tibias and femurs of the guinea pigs. The third passage BMMSCs were cultured with plasmid-lipofectamine mixture for 6 hours, followed by fetal bovine medium for 48 hours. Immunohistochemistry was performed for transient expression. G418 was added after 48 hours.

①脂质体法：取体外分离、培养的第3代豚鼠骨髓间充质干细胞，将质粒-脂质体混合物加入含细胞的培养基中培养6 h，再加入胎牛血清的培养基，孵育48 h 后行免疫组织化学检测，即为瞬时表达。48 h后加入含G418培养基筛选。

You have isolated a plasmid DNA that is a closed circular molecular 1050 bp in length with 5 negative supercoils.

你分离出的质粒 DNA 为1050 bp 长度的共价闭合环状分子，具5个负超螺旋。

You have isolated a plasmid DNA that is a closed circular molecular 1050 bp in length with 5 negative supercoils.

请问这个DNA分子的连接数、螺旋数、扭曲数、以及超螺旋密度是多少？

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！