查询词典 plasmid

Objective To construct the eucaryotic recombinant plasmid of pYES2/LactoferricinB expressing in yeast of S. cerevisiae, of which the expressed protein antibacterial activity was verified in preliminary.

目的 构建牛乳铁多肽的真核表达质粒pYES2/Lactoferricin B，实现其在酿酒酵母S.cerevisiae中的表达，并初步检测其不同变异的体外抗菌活性。

Objective To construct recombination eukaryon expression plasmid for human parathyroid hormone gene, assay PTH expression and biological activity after transfection in vitro and evaluate gene therapy effect on hypoparathyroidsm.

目的构建甲状旁腺激素基因的重组真核表达质粒，评价体外转染后PTH基因的表达与生物学活性，同时观察其对甲状旁腺功能减退动物的基因治疗作用。

Methods:eukaryon expression vector pEGFPN1-NT-3 recombinant plasmid wasconstructed and transfected into NSCs to establish EGFP-labeled NT-3 gene modifiedNSCs via nucleofection.

构建NT-3基因真核表达载体pEGFPN1-NT-3后，应用核转染技术将其转入神经干细胞内建立NT-3基因修饰的神经干细胞。

The shRNA eukaryon expression plasmid vectors targeting rat Pik3cb were constructed using VSMC-specific SM22α promoter.

采用血管平滑肌细胞特异性SM22α启动子构建靶向大鼠Pik3cb基因的干扰RNA真核表达载体，既可抑制新生内膜增生，又可防治血栓的形成。

The method was to set up a freezing time ladder of the mixture of competented cell and plasmid along with a negative sample to research under the same condition of other aspects;The results revealed that the transforming efficiency was the highest at the freezing time of 20 minutes;Compared with the efficiency of other freezing times,the 20 minutes freezin...

在保证其他条件一致的情况下，设置大肠杆菌感受态细胞和质粒混合物的冰浴时间梯度并建立阴性对照进行研究。结果：冰浴20 min时转化效率最高。结论：与其他冰浴时间相比，冰浴20 min时的转化效率最高，实验重复性好，成本低廉，而且操作简便，值得推广使用。

The aim of the research was to confirm the best freezing time of the mixture of competented cell and plasmid in the E.coli DH5α CaCl2 transforming method.

确定大肠杆菌DH5α氯化钙转化法时感受态细胞与质粒混合物的最佳冰浴时间。

The full hBD2 cDNA was inserted into the multi clone site of a eukaryotic expressive plasmid pcDNA3.1/Myc His and located closely at the upstream of two tag gene (myc and 6 Poly histidines ) so as to construct another recombinant eukaryotic expressive vector of hBD 2 gene: rpcDNA3.1/Myc Hi...

将 h BD- 2的全长 c DNA片段插入编码双重报告基因 Myc和 6个多聚组氨酸的真核表达质粒 pc DNA3.1/Myc- His ，构建出 C端带 Myc和 6× His的 rpc DNA3.1/Myc-His /h BD- 2。

Comparison of the coding regions of BH1-BH4 genes showed that BH1 had a lower sequence identity to other previously published B-hordeins than the other three B-hordeins obtained in this study. BH1 was then cloned in a bacterial expression vector based on bacteriophage T7 RNA polymerase. The resulting plasmid produced a 28.15 kDa protein in Escherichia coli. The potential value of B-hordein genes in grain quality improvement of hull-less barley has been discussed.

并且，在4个基因BH1～BH4中，BH1与先前报道的B组醇溶蛋白基因有较低的序列相似性，因此我们对BH1基因进行了原核表达，含该基因的表达载体在大肠杆菌中表达出相对分子质量为28.15 kDa并以包涵体形式存在的蛋白，进一步对其在青稞谷粒品质改良中的潜在价值进行了探讨。

They also found that "CRISPR interference," as this phenomenon is known, involves the targeting of the incoming plasmid or virus DNA directly.

他们也发现了CRISPR干扰作用，正如被认识的一样，该作用包括间接将进入的质粒或者病毒DNA作为目标。

We used a reporter gene plasmid in which firefly luciferase expression is dependent on the HCV IRES.

我们使用了依赖HCV IRES表达萤火虫荧光素酶的报告基因质粒。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！