查询词典 plasmid

By means of experiments of marker transfer,acridine orange sensitivity test,F' curring and mini-chromosome transformation it is.conchi-ded that the F' plasmid is always in an integrated state in the Sin strains and that the initiation of the replication of the bacterial chromosome is carrie...

标记转移、吖啶橙敏感性，F′质粒消除和mini-染色体质粒转化等实验说明，Sin菌株中F′质粒始终处于整合状态，并且在40℃中细菌染色体的复制由整合状态的F′质粒所带动。

A recombinant pl asmid (designated as p8.0) was obtaining by ligating these three fragments. The p8.0 was subcloned into a plasmid (designated as p8.2) containing entire LTR of EIAV DLA. The complete nucleotide sequence of DLA strain of EIAV was determined by sequencing the p 8.2 . The purified p8.2 was used to transfect donkey leuko cyte cultures.

将此8.0kb EIAV全基因再亚克隆到含有一完整EIAV DLA株长末端重复序列的质粒中，获得一含有EIAV驴白细胞弱毒前病毒全基因的重组质粒，将其命名为p8.2，经核苷酸序列分析，证明p8.2含有EIAV前病毒的全基因。

Results The bcl-2 gene identified by the colony PCR was further confimed by PCR with plasmid DNA and digestions as well as DNA sequencing.

结果在筛选的阳性克隆中扩增到大鼠Bcl-2基因，经菌落PCR、酶切鉴定及DNA序列分析证实该序列正确。

Screening and identification: the resulting plasmid was extracted by alkali division, identified by restriction enzyme digestions and PCR, observed by 1% agarose gels electrophoresis.

为了提高了目的片段和载体之间的连接效率以及目的片段的拷贝数，本实验所选用的载体是pMD18-T Vector，它是由pUC18用EcoRⅤ切割后在两个3'末端加上单个的碱基'T'而制得的线状分子。

The amplified fragment was inserted into pRSET-A to generate the first double repeat of the precursor gene. By utilizing a pair of isocaudamer BamH I and Bgl II sites, and another downstream Hind III site of plasmid pRSET-A, following a series of simple double digestions and ligation of the resulted products, a series of repeat (3, 4 and 6) precursor peptide fragment genes were derived.

本实验设计一对两侧含编码疏水性氨基酸密码子的引物，经过扩增前导序列10~34aa基因序列，并重新克隆入质粒pRSET-A构建串联二聚体后，再利用质粒pRSET-A的BamH I / Bgl II同尾酶克隆位点，经一系列简单的酶切和连接，快速构建这一前导肽中不含组氨酸标签序列的串联多聚体基因，并成功表达其六聚体重组蛋白。

After introduction of the recombinant plasmid into BME cells, the capability of proliferation was obviously enhanced and the DNA content was changed, the cell proliferation lifespan was elongated to 13 population doublings to control.

导入STAT3基因后，细胞增殖能力增强，染色体倍数发生变化，细胞的增殖寿命较对照细胞延长了13代，表明导入STAT3基因后，能延长体外培养的牛乳腺上皮细胞的寿命。

Objective To construct a plasmid vector with EGFP reporter gene for functional analysis of enhancers.

目的 构建以EGFP为报告基因的增强子鉴定质粒载体，并对其进行鉴定。

It is the Agrobacterium to use different strain with plasmid pCAMBIA 1201 or 1303, including transformation research for EHA105, LBA4404 and KYRT1, try to find out the relevant condition that the suitable azuki bean epicotyls transfer to transformation.

使用带有pCAMBIA1201或1303质体的不同菌系农杆菌，包括分别为EHA105、LBA4404及KYRT1进行转殖研究，尝试找出适合红豆胚轴转殖的相关条件。

Full length LMP2A cDNA was firstly incised from pGEM-T-LMP2A with EcoR Ⅰ, Sma Ⅰ digestion, and then inserted into eucaryote. expression plasmid pCIcc controlled by CMV promoter. The CMV-LMP2A-SV40 expression unit was digested by ClaI, and inserted into E1-substituted adenovirus vector pAx1cw. Then the LMP2A recombinant adenovirus vector was cotransfected into 293 cells together with EcoT22I digested Ad5-TPC.

将带有LMP2A cDNA的重组质粒pGEM-T-LMP2A用EcoR Ⅰ、Sma Ⅰ双酶切下LMP2A cDNA，并将其插入含同样酶切位点的真核表达质粒pCIcc中，使其受控于CMV启动子下；用ClaI切下CMV-LMP2A-SV40表达单元，插入E1、E3区替代的腺病毒载体pAX1CW，选择正确的克隆pAX1CW-LMP2A与Ad5 DNA-末端肽复合体共转染293细胞，通过同源重组获得复制缺陷型的重组腺病毒(Ad5-LMP2A)。

Objective To construct the eucaryotic recombinant plasmid of pYES2/LactoferricinB and express in yeast of S.cerevisiae and preliminary verify the antibacterial activity.

目的构建牛乳铁多肽的真核表达质粒pYES2／Lactoferricin B，实现其在酿酒酵母S.cerevisiae中的表达，初步检测其不同变异的体外抗菌活性。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！