查询词典 plasmid

The resultant recombinant plasmid, terming pMB1, was transformed into E. coil BL21(DE3). IPTG was used to induce the expression of the target protein.

将重组质粒转化到宿主菌BL21 (DE3)中，对重组菌株BL21(pMB1)用IPTG进行诱导表达，并对最佳诱导时间进行了探索。

The DNA sequences corresponding to the 3'terminuses of HCV genotype 1a, 1b and 2a negative RNA strand were cloned to the transcription plasmid pGEM3Zf.

以1a型负链3'末端RNA为模板，NS5B1和NS5B2为聚合酶，进行体外的RNA合成反应，斑点杂交和RT-PCR检测产物，确定纯化的蛋白具有RdRp活性。

This P element then transposes from the plasmid to a random chromosomal site.

携带目的基因的P因子可从质粒转座到任意染色体上。

BALB/c mice were immunized with the plasmid truncates and challenged with homologous virus at a lethal dosage.

通过观察小鼠的存活率和检测血清中抗NA抗体的效价以及肺部病毒量来寻找NA抗流感病毒的关键序列。

Methods PCR amplification of drug resistance gene abeM was carried out using chromosomal DNA of Ab 35 and Ab 36 clinical isolates as template. PCR product of abeM gene was sequenced. The nucleotide sequences and deduced amino acid sequences were analyzed by BLAST of NCBI. After cells of Escherichia coli KAM32 transformed with a plasmid carrying the abeM gene PCR product, MICs of various drugs were determined by serial twofold dilution method.

以临床分离的Ab 35、Ab 36染色体DNA为模板扩增abeM基因，对abeM基因扩增产物进行DNA测序及同源性分析；将abeM基因扩增产物与载体连接后转化入大肠埃希菌KAM32感受态细胞中，对转化子进行药敏分析。

Schistosoma japonicum ; Fatty acid-binding protein ; Recombinant plasmid ; Expression

日本血吸虫；脂肪酸结合蛋白；重组质粒；表达

METHODS: BMSCs were obtained from Japanese big-eared rabbits, and in vitro cultured. Then the subcultured BMSCs were transfected by pCDNA3.1 plasmid, followed by incubation on swine SIS to construct the tissue-engineered skin. The growth of cells and phenotype of BMSCs were detected by flow cytometry.

日本大耳兔骨髓间充质干细胞进行体外培养扩增后，通过pCDNA3.1质粒将碱性成纤维细胞生长因子基因转染至生长状态良好的骨髓间充质干细胞，并将转染后的细胞接种于制备好的猪小肠黏膜下层上，进行体外联合培养，构建组织工程皮肤。

University of Tokyo , Tokyo 11328657 , JapanAbstract : The genome DNA was extracted from the blood of six horse breeds including SH , B H ,XN , WZ , TB , AI. Four pair of primers were designed based on the sequence of human , mouse and ferret DRD4 gene cDNA to amplify the corresponding horse gene. The PCR products were ligated into a linear plasmid pMD17 , and sequenced respectively.

从6 个品种马的血液中提取基因组DNA ，根据GenBank中已发表的人、鼠、雪貂的DRD4 cDNA 基因保守区序列，设计3 对特异性引物，另1 对由文献所得，对6个品种马的DRD4 基因分4 段进行PCR 扩增，经电泳检测，分别呈4条特异条带，将其分别克隆入pMD192T 载体中，进行序列测定并拼接序列，得到包括所有内含子的DRD4 基因序列。

The total RNA of viruses isolated was extracted from the Hantavirus-infected Vero-E6 cells and the S-segment cDNA of Hantavirus was obtained by RT-PCR. This gene fragment was then cloned into plasmid vector pUCm-T and sequenced afterwards by using the DNA STAR software for comparison.

提取病毒总RNA，应用逆转录聚合酶链反应扩增病毒S基因全片段，克隆入质粒载体，进行核苷酸序列测定及分析，应用DNA STAR软件分析比较，确定病毒型别。

Objective:To construct the recombinant adenovirus plasmid carring human PML gene for further study.

目的：构建携带PML目的基因的重组腺病毒载体。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！