查询词典 plasmid

Objective To construct the recombinant plasmid harbouring the encoding gene of Mus musculus metallothionein Ⅱ.

目的 构建含有小鼠Ⅱ型金属硫蛋白基因的真核表达载体，为进行MT抗氧化作用的体内功能实验奠定基础。

Some of them were ligated to the plasmid vector pCR 2.1 andsequenced,respectively.

将所获DNA片段克隆到pCR2.1载体进行测序，并与基因组数据库进行同源性比较。

The recombinant plasmid was constructed by ligating the PCR product and pUC18 in vitro and then transformed into E.

将PCR产物和pUC18在体外进行连接后，并分别转入E。

Necropsies were performed at 21 days post-challenge, the lymph nodes in the nonvaccinated but challenged pigs were larger than those of vaccinated pigs. The PCV2 genomic copy loads in lymph nodes were reduced in vaccinated pigs. Moderate amounts of PCV2 antigen were detected in most lymphoid tissues of nonvaccinated pigs. These data indicated that when given intramuscularly in pigs, the chimeric PCV1-2 live virus and recombinant plasmid induces protective immunity against PCV2 infection and could potentially serve as an effective vaccine.

攻毒后21天攻毒对照组猪淋巴结比免疫组猪显著肿大，免疫组猪血清、淋巴结中PCV2病毒载量低于对照组，攻毒对照组猪淋巴结中PCV2抗原含量高于免疫组，这些结果表明嵌合型猪圆环病毒PCV1-2及真核表达质粒肌肉注射免疫商品猪后，对PCV2感染能产生保护性免疫应答，有可能成为候选疫苗。

The expression plasmid of hIL-3 gene nuder the COntrol ...

构建的PL启动子控制下的hIL－3cDNA表达质粒，转入大肠杆菌Tap106，经42℃热诱导，获得hIL－3的表达产物。

The expression plasmid of hIL-3 gene nuder the CO...

构建的PL启动子控制下的hIL－3cDNA表达质粒，转入大肠杆菌Tap106，经42℃热诱导，获得hIL－3的表达产物。

Methods: The medicinal plant Rheum wittrochii Lundstr., was genetically with Ri plasmid harbouring in Agrobacterium rhizogenes 9402 strain to induce hairy roots; transformed the opines from the hairy roots were assayed by paper electrophoresis; the contents of free anthroquinones such as aloe-emodin, rhein, chrysophanol, emodin, physcion, 8-O-methylchrysophanol from the hairy roots were quantitatively determined by HPLC technique.

用发根农杆菌Agrobacterium rhizogenes 9402 所含Ri质粒遗传转化药用植物天山大黄以诱导发根用纸电泳法检测发根中冠瘿碱的存在；用高效液相色谱法测定发根中芦荟大黄素、大黄酸、大黄酚、大黄素、大黄素甲醚、大黄酚-8-甲醚等游离蒽醌化合物的含量。

The foreign DNA is spliced into a plasmid, which is taken up by the bacterium.

在植物细胞 DNA 转导中常用的载体是一种细菌农杆菌 Agrobacterium tumefascients。

The plasmid pcDNA3.1-3-1E and pcDNA3.1 was respectively used to immunize SPF chicks by injection intra-muscularly in thigh muscle at 14 and 21 days of age.Chicks in each group except that in the unchallenged control group were challenged with 5×104 E.acervulina sporulated oocysts at 28 days post-inoculation,and the immune protective efficacy was observed.

将构建的重组质粒以及空载体质粒分别于14、21日龄分2次经腿部肌肉注射免疫SPF雏鸡，28日龄除非免疫非感染对照组外各组攻击性感染5×104个堆形艾美球虫孢子化卵囊，观察真核表达质粒对球虫感染鸡的免疫保护作用并探讨其免疫保护机理。

Objective To study the antibioticsusceptibility of 693 strains of bacteria and its relation to the plasmid.

目的 研究693株细菌的药物敏感状况与其质粒的关系。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。