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Na-K-Exchanging ATPase is a universal cell membrane protein in higher eukaryotic cells which mediates the anti-concentration gradient exchange of intracellular Na+ for extracellular K+. It plays essential metabolic roles such as maintaining sodium and potassium ion gradients across the plasma membrane. The targeted sequences were amplified by PCR to determine the genome structure of the hithertofore unsequenced portion of the alpha 1 subunit of the human Na-KExchanging ATPase gene which encodes 80-130 aa of its extracellular domain using two different templates, human genomic DNA and a human muscle cDNA library. The PCR products were analyzed by restriction endonuclease digestion and then cloned into a plasmid vector for chemiluminescence sequencing and further analysis.

Na-K-Exchanging ATPase是一种普遍存在于高等生物体内的细胞膜蛋白，主要参与介导K+和Na+在细胞内外之间的逆浓度梯度的转运，并维持一定细胞内外的离子梯度，我们采用聚合酶链式反应方法分别以人基因组DNA及cDNA文库为模板对人Na-K-Exchanging ATPaseα1亚单位基因胞外区约80-130位氨基酸编码序列进行扩增，限制性酶切分析扩增产物，并进行荧光测序，对测序结果进行同源性分析及剪接位点的搜索并对得到的核苷酸序列进行进一步的分析，发现人基因组DNA和cDNA经过扩增后分别得到833bp和195bp两种不同大小的片段Fg，Fc。

We also construct the plasmid that can express the three protein to verificate whether they can interact with BACE1 in mammalian cells.

我们构建了可以在哺乳动物细胞中表达这三个蛋白的载体。

Expression of Bilirubin Oxidase cDNA from Myrothecium Verrucaria in E. coliThe 1. 6kb bilirubin oxidase cDNA from Myrothecium Verrucaria was excised from plasmid pGEM-T/BOX, and inserted into expression vector, pET-3a which were under the control of a T7 promoter.

用限制性内切酶将1.6kb疣疱漆斑菌胆红素氧化酶cDNA从质粒pGEM-T／BOX切下，插入到表达质粒pET-3a的T7启动子下游，构建成疣疱漆斑菌胆红素氧化酶表达质粒pET-3a／BOX。

The results showed that the reporter plasmid could be used to identify the genes which positively regulate the expression of hrpX in Xanthomonas campestris.

实验结果表明该报告质粒可用于筛选鉴定正向调控野油菜黄单胞菌hrpX表达的基因。

In vitro associstion experiments indicate that the plasmid pAy6.8 containing the core region of Antheraea yamamai fibroin gene can tightly bind to the nuclear matrix of Bombyx mori fertilized eggs,and recognize the same binding sites of homogeoous MAR fragments.

体外结合实验表明，携带天蚕丝素基因核心区的质粒pAy6.8可与家蚕受精卵核基质紧密结合，而且同内源MAR片段竞争结合位点。

Objective To develop a PCR-based method for rapid and effective screening of arrayed plasmid cDNA library.

目的 建立一种快速、有效地筛选质粒cDNA文库的方法―PCR法。

Kanic acid was injected into guinea pigs' cochleae and the excitotoxicity model was established. After a week the recombinant plasmid was transferred into SGCs of guinea pigs' cochlea treated with HAT. The following week the expression of NT-3 was examined by the immunohistochemical method, and the morphology of SGNs was observed under the electronic microscope after 4 weeks, in the mean time the changes of auditory brain-stein response were examined.

通过耳蜗灌注海人酸(kainic acid， KA)建立豚鼠耳蜗兴奋性损伤模型，在给KA 1周后利用HAT携带重组质粒进行耳蜗灌注以转染耳蜗螺旋神经节细胞，免疫组化法观察转染后1周NT-3的表达及4周后电镜下螺旋神经节细胞形态学变化，同时观察对听觉脑干诱发电位(auditory brain-stem response， ABR)的影响。

The crude DNA pellets were recovered by dialyzing and then could be used for molecular operation such as amplification of 16S rRNA gene and digestion of restriction enzymes.The plasmid pUC19 was prepared and digested with BamHI.

粗提DNA用半透膜透析回收纯化，纯化后的DNA可以进行16sRNA基因PCR扩增，酶切等分子生物学操作，具有较高的质量。

The P32 gene of capripoxvirus were amplified by PCR.The product of PCR was cloned to the plasmid pGEM-T-Easy.Then the P32 gene was disparted to three parts.Three parts of P32 gene were inserted into the expressed plasmids pGEX-4T-1, which were identified by PCR and restriction enzyme analysis.

本研究利用PCR方法对羊痘病毒P32蛋白基因扩增，将回收PCR产物克隆到pGEM-T-Easy载体中，然后将P32蛋白基因分成3段，分别插入原核表达载体pGEX-4T-1中，经PCR和酶切鉴定，证明成功构建了重组表达载体pGEX-4T-1-N2/3、pGEX-4T-1-N1/3、pGEX-4T-1-M。

A modified method for isolating mitochondrial plasmid-like DNA from etiolated plant seedlings was set up at first.

本文在文献报道的实验方法基础上，建立了一种简便、快捷、有效的提取植物线粒体DNA类质粒的方法。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！