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The 682-bp fragment of human IFN-〓 cDNA was inserted into theXhoI site of the BMGNeo vector containing a neomycin resistancegene.The plasmid containing the translationally oriented IFN-〓cDNA insert (BMGNeo-IFN-〓) was selected by restriction enzymeanalysis.

将682bp的全序列人〓 cDNA插入到携有〓基因的表达载体BMGNeo的Xho I位点上，对此重组表达载体进行了限制性酶切鉴定。

In this sense, IDA plays dual effects, tridentate chelating to Cu(2) and bridgebetween two Cu(1) andCu(2).3 Metal complexes were selected as the appropriate for the study of cleavage plasmid pBR322DNA by gel electrophoresis technique. The results showed Ni and Mn complexes could cleave effect- ively DNA in the presence of H2O2 at physiological pH and temperature, whereas individual Zn complex could cleave effectively DNA.

通过电泳实验研究了一系列金属配合物与pBR322DNA的作用，发现在Tris-HCl缓冲溶液中，生理条件下，镍、锰配合物在共反应物H_2O_2存在下能够很好的断裂DNA，而Zn配合物单独作用就能够使DNA由ccc型转化为oc和linear型。

Several important parents of hybrid rice were used in this study and transferred with the grobacterium tume faciens strain EHA105 harboring Ti plasmid pCAMBIA1301, which contains a hygromycin resistant gene as the selectable marker and a β-glucuronidase gene as the reporter gene.

选用我国籼型杂交稻中的几个重要亲本，以携带有双元载体pCAMBIA1301的根癌农杆菌EHA105为载体，以GUS基因的瞬间表达为依据，研究根癌农杆菌介导转化籼稻的影响因素，确立对转化频率有重要影响的几个条件。

This implies that DNA methylation of promoter exon 1_7 may not underly the mechanism of GR up-regulation in GLP-1 -programmed rats.5 Hippocampal expression of GLP-1R and NGFI-A mRNAHippocampal GLP-1R (p =.009) and NGFI-A (p =.001) gene mRNA expression were significantly up-regulated in GP group as compared to VP group.Taken together, neonatal intramuscular injection of plasmid DNA encoding GLP-1 affects behavior and hippocampal GR expression in adolescent rats.

提示GR exon 1_7启动子区域DNA甲基化可能不参与GLP-1导致的GR转录上调。6海马GLP-1R和NGFI-A mRNA表达GP组大鼠GLP-lR(P=。009)和NGFI-A(P=。001)表达均显著高于VP组，表明肌肉注射GLP-1可能通过作用于其海马受体提高NGFI-A表达，进而调控GR表达。

Chicken IL-15(ChIL-15), Which was discovered in 2001, plays a main role in activating NK cells and CD8+ memory T cells, and may therefore have potential as a vaccine adjuvant.According to published ChIL-15 gene sequence, a pair of primers were designed and synthesized to clone ChIL-15 cDNA from ConA-activated chicken splenocytes by RT-PCR. Recombinant plasmid pUC19ChIL-15 carrying the ChIL-15 gene was constructed. The gene encoding mature ChIL-15 (mChIL-15) was amplified from pUC19ChIL-15 by PCR and cloned into pMD 18-T vector. After digested with Kpn I /Pst I , the mChIL-15 gene was subcloned into prokaryotic expression vector pPRoEX橦Ta and transformed into E.coli DH5a .The transformant was induced by IPTG, and the recombinant ChIL-15(rChIL-15) was expressed as a fusion protein with a histidine hexamer tag at the N-terminal end of the protein. Following expression, the protein was purified by ProBond?Nickel-Chelating Resin. The uninduced and induced protein lysates and the purified protein were separated by SDS-PAGE and transferred onto nitrocellulose membrane to identify rChIL-15 by Western blot. The rChIL-15 was used to immune the guinea pig to obtain rChIL-15 polyclonal antibody.

参考已发表的ChIL-15基因序列，设计合成引物，应用RT-PCR技术从刀豆球蛋白A活化的白来航鸡脾淋巴细胞中克隆ChIL-15 cDNA，将其与SmalⅠ酶切处理的pUC19载体连接，构建重组质粒pUC19ChIL-15；用PCR技术从pUC19ChIL-15中扩增出去信号肽的成熟ChIL-15(mChIL-15)基因，与pMD 18-T载体相连构建重组质粒pMDChIL-15；然后用KpnⅠ／PstⅠ双酶切下mChIL-15基因片段，定向亚克隆到经同样酶切处理的带有6个组氨酸标签的表达载体pP_RoEX~HTa中，构建重组质粒pP_RoChIL-15，对其测序确认读框正确后，转入大肠杆菌DH5α中诱导表达并进行纯化，用重组ChIL-15(rChIL-15)免疫豚鼠，制备多克隆抗体；再用HindⅢ／PstⅠ从质粒pP_RoChIL-15上切下mChIL-15基因，定向亚克隆到经同样酶切处理的杆状病毒转移载体pMelBacB中，经酶切、PCR和序列测定鉴定后，与杆状病毒线形化DNABac-N-Blue~(TM DNA共转染Sf9昆虫细胞，经三轮蚀斑纯化，构建重组杆状病毒rBac-ChIL-15，用该重组病毒感染处于对数生长期的Sf9细胞，按不同的时间收取样品，离心后对其上清和沉淀进行SDS-PAGE和Western blot分析。

After a single intramuscular injection of plasmid hlL-6 Cdna on 6.5 Gy-irradiated mice, the IL-6 level showed less changes in unirradiated mice.

经6.5 Gy照射的小鼠一次肌内注射表达IL-6的质粒DNA后，用ELISA方法检测血浆IL-6水平，以蛋白表达量评价基因转移效率。

The recombinant plasmid DNA of PrM-E gene but not unmethylated CpG was capable of inducting antibodies against D2-43 virus.

我国登革2型心理病毒 PrM-E基因重组质粒DNA具有一定的免疫原性。

The recombinant plasmid DNA of PrM-E gene but not unmethylated CpG was capable of inducting antibodies against D2-43 virus.

我国登革2型病毒PrM-E基因重组质粒DNA具有一定的免疫原性。

The recombinant plasmid DNA of PrM-E gene but not unmethylated CpG was capable of inducting antibodies against D2-43 virus.

我国登革2型病毒PrM-E基因重组质粒DNA具有一定的免疫原性。在本实验条件下，含非甲基化细菌性CpG的pUC19质粒DNA，对PrM-E基因的DNA免疫效果没有促进作用。

Methods The recombinant plasmid of CPB, pET-21a-CPB, was constructed and transformed into E. coli BL21(DE3). The expression was induced in 25℃and 37℃, respectively. The obtained inclusion bodies were dissolved in different denaturing solution systems. The protein concentration was determined in order to indicate the solubility of CPB inclusion bodies in the denaturing solution. And probable mechanism for the solubility differences was illuminated by the analysis of unreduced SDS-PAGE.

方法构建CPB重组质粒pET-21a-CPB，将其导入表达菌株BL21（DE3）中，分别在25 ℃和37℃进行诱导表达；所得包涵体分别用不同浓度尿素添加不同还原剂溶解，以溶液中的蛋白质浓度判定其溶解性，利用非还原SDS-PAGE分析其溶解性提高的原因。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！